Analyse chicken data starting from raw data to clustering results.
NB. run in Seurat 3.1.5
Load data.
library(Seurat)
library(dplyr)##
## Attaching package: 'dplyr'## The following objects are masked from 'package:stats':
##
## filter, lag## The following objects are masked from 'package:base':
##
## intersect, setdiff, setequal, unionlibrary(cowplot)##
## ********************************************************## Note: As of version 1.0.0, cowplot does not change the## default ggplot2 theme anymore. To recover the previous## behavior, execute:
## theme_set(theme_cowplot())## ********************************************************library(patchwork)##
## Attaching package: 'patchwork'## The following object is masked from 'package:cowplot':
##
## align_plotslibrary(scrattch.hicat)setwd('~/postdoc2/DCN_sequencing/chicken/plateseq/novaseq43/')
raw_counts68e<-read.table(file="genesXcells_exons_LLL68.tab",sep="\t",row.names=1,header=TRUE)
raw_counts69e<-read.table(file="genesXcells_exons_LLL69.tab",sep="\t",row.names=1,header=TRUE)
raw_counts70e<-read.table(file="genesXcells_exons_LLL70.tab",sep="\t",row.names=1,header=TRUE)
raw_counts71e<-read.table(file="genesXcells_exons_LLL71.tab",sep="\t",row.names=1,header=TRUE)
raw_counts72e<-read.table(file="genesXcells_exons_LLL72.tab",sep="\t",row.names=1,header=TRUE)
raw_counts73e<-read.table(file="genesXcells_exons_LLL73.tab",sep="\t",row.names=1,header=TRUE)
raw_counts74e<-read.table(file="genesXcells_exons_LLL74.tab",sep="\t",row.names=1,header=TRUE)
raw_counts75e<-read.table(file="genesXcells_exons_LLL75.tab",sep="\t",row.names=1,header=TRUE)
raw_counts76e<-read.table(file="genesXcells_exons_LLL76.tab",sep="\t",row.names=1,header=TRUE)
raw_counts77e<-read.table(file="genesXcells_exons_LLL77.tab",sep="\t",row.names=1,header=TRUE)
raw_counts68i<-read.table(file="genesXcells_introns_LLL68.tab",sep="\t",row.names=1,header=TRUE)
raw_counts69i<-read.table(file="genesXcells_introns_LLL69.tab",sep="\t",row.names=1,header=TRUE)
raw_counts70i<-read.table(file="genesXcells_introns_LLL70.tab",sep="\t",row.names=1,header=TRUE)
raw_counts71i<-read.table(file="genesXcells_introns_LLL71.tab",sep="\t",row.names=1,header=TRUE)
raw_counts72i<-read.table(file="genesXcells_introns_LLL72.tab",sep="\t",row.names=1,header=TRUE)
raw_counts73i<-read.table(file="genesXcells_introns_LLL73.tab",sep="\t",row.names=1,header=TRUE)
raw_counts74i<-read.table(file="genesXcells_introns_LLL74.tab",sep="\t",row.names=1,header=TRUE)
raw_counts75i<-read.table(file="genesXcells_introns_LLL75.tab",sep="\t",row.names=1,header=TRUE)
raw_counts76i<-read.table(file="genesXcells_introns_LLL76.tab",sep="\t",row.names=1,header=TRUE)
raw_counts77i<-read.table(file="genesXcells_introns_LLL77.tab",sep="\t",row.names=1,header=TRUE)setwd('~/postdoc2/DCN_sequencing/chicken/plateseq/novaseq44/')
raw_counts78e<-read.table(file="genesXcells_exons_LLL78.tab",sep="\t",row.names=1,header=TRUE)
raw_counts78i<-read.table(file="genesXcells_introns_LLL78.tab",sep="\t",row.names=1,header=TRUE)setwd('~/postdoc2/Gedankenpapers/CNevomanuscript/code/snRNAseq/chicken/')
raw_counts_e<-cbind(raw_counts68e,raw_counts69e,raw_counts70e,raw_counts71e,
raw_counts72e,raw_counts73e,raw_counts74e,raw_counts75e,raw_counts76e,raw_counts77e,raw_counts78e)
raw_counts_i<-cbind(raw_counts68i,raw_counts69i,raw_counts70i,raw_counts71i,
raw_counts72i,raw_counts73i,raw_counts74i,raw_counts75i,raw_counts76i,raw_counts77i,raw_counts78i)
# create a new variable from the rownames
# add rownames as a column in each data.frame and bind rows
counts<-bind_rows(raw_counts_e %>% add_rownames(),
raw_counts_i %>% add_rownames()) %>%
# evaluate following calls for each value in the rowname column
group_by(rowname) %>%
# add all non-grouping variables
summarise_all(sum)## Warning: Deprecated, use tibble::rownames_to_column() instead.
## Warning: Deprecated, use tibble::rownames_to_column() instead.counts<-as.data.frame(counts)
rownames(counts)<-counts[,'rowname']
badrows1=grep("__",rownames(counts))
badrows2=grep("ERCC-",rownames(counts))
counts<-counts[!rownames(counts) %in% c(rownames(counts)[badrows1],rownames(counts)[badrows2]),2:dim(counts)[2]]replace known ENSGALG genes with their names, keep the remainder
#load conversion table
conversiontable=read.csv("data/EnsembleID99_to_GeneSymbol.txt")
conversiontable.df<-as.data.frame(conversiontable)
conversiontable.notrepeated<-conversiontable[!(duplicated(conversiontable[,2])|duplicated(conversiontable[,2], fromLast=TRUE)),]
rownames(conversiontable.notrepeated)<-conversiontable.notrepeated[,1]
counts_translated<-counts[rownames(counts) %in% rownames(conversiontable.notrepeated),]
rownames(counts_translated)<-as.character(conversiontable.notrepeated$Gene.name[match(rownames(counts_translated),conversiontable.notrepeated$Gene.stable.ID)])
counts_nottranslated<-counts[!rownames(counts) %in% rownames(conversiontable.notrepeated),]
counts2<-rbind(counts_translated,counts_nottranslated)
rm(list=setdiff(ls(), "counts2"))N44<- CreateSeuratObject(counts2, min.cells = 0, project = "DCN_plateseq")## Warning: Feature names cannot have underscores ('_'), replacing with dashes
## ('-')#ERCC <- grep(pattern = "^ERCC", x = rownames(x = N44@assays$RNA@data), value = TRUE)
#N44 <- AddMetaData(object = N44, metadata = colSums(N44@assays$RNA@data[ERCC,])/colSums(N44@assays$RNA@data), col.name = "ERCC")
VlnPlot(N44,c("nCount_RNA","nFeature_RNA"))## Warning: Removed 11 rows containing non-finite values (stat_ydensity).## Warning: Removed 11 rows containing missing values (geom_point).## Warning: Removed 11 rows containing non-finite values (stat_ydensity).## Warning: Removed 11 rows containing missing values (geom_point).
annotate by animal:
plates=sapply(strsplit(rownames(N44@meta.data),"_"),function(x) x[1])
C3=plates %in% c("LLL68","LLL69","LLL70","LLL78")
C4=plates %in% c("LLL71","LLL72","LLL77")
C5=plates %in% c("LLL73","LLL74","LLL75","LLL76")
donor=as.factor(as.numeric(C3)+2*as.numeric(C4)+3*as.numeric(C5))
levels(donor)<-c("Chicken3","Chicken4","Chicken5")
N44$donor<-donorannotate by FACS round:
plates=sapply(strsplit(rownames(N44@meta.data),"_"),function(x) x[1])
R1=plates %in% c("LLL68","LLL69","LLL70","LLL78")
R2=plates %in% c("LLL71","LLL72","LLL77")
R3=plates %in% c("LLL73","LLL74","LLL75","LLL76")
FACS=as.factor(as.numeric(R1)+2*as.numeric(R2)+3*as.numeric(R3))
N44$FACS<-FACSN44 <- NormalizeData(object = N44, normalization.method = "LogNormalize", scale.factor = 1e6)
N44<- subset(x = N44, subset = nFeature_RNA > 1000)
N44 <- FindVariableFeatures(object = N44,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
N44 <- ScaleData(object = N44,vars.to.regress = c('nCount_RNA'))## Regressing out nCount_RNA## Centering and scaling data matrixN44 <- RunPCA(object = N44,npcs = 30,verbose = FALSE)VlnPlot(object = N44, features = c("nFeature_RNA", "nCount_RNA"), ncol = 2)
#N44 <- JackStraw(object = N44, dims=20)
#N44<- ScoreJackStraw(N44,dims=1:20)
#JackStrawPlot(object = N44,dims=1:20)ElbowPlot(object = N44)
N44<- FindNeighbors(N44,dims=1:20)## Computing nearest neighbor graph## Computing SNNN44 <- FindClusters(object = N44, resolution = 1)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 3407
## Number of edges: 130352
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.8399
## Number of communities: 18
## Elapsed time: 1 secondsN44 <- RunTSNE(object = N44, dims = 1:20, perplexity=30, dim.embed = 2)
p1<-DimPlot(object = N44, reduction = 'tsne', label=TRUE)
p2<-DimPlot(N44,group.by = 'donor')
p4<-DimPlot(N44,group.by = 'orig.ident')
plot_grid(p1,p2,p4,ncol=2)
FeaturePlot(object = N44, features = c("SNAP25","SLC17A6","SLC6A5","GAD1","ETV1","RBFOX3"), reduction= "tsne")
FeaturePlot(object = N44, features = c("GAD1","GAD2","PCP2","CALB1","GLUD2"), reduction= "tsne")## Warning in FetchData(object = object, vars = c(dims, "ident", features), : The
## following requested variables were not found: PCP2, GLUD2
table(N44$RNA_snn_res.1)##
## 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
## 611 397 333 321 253 211 195 188 171 154 143 93 91 85 62 46 29 24VlnPlot(N44,c("nFeature_RNA","nCount_RNA"))
non-neuronal cells: pdgfra(OPC),slc14A1(astrocyte),Gfap(astrocyte),Opalin(oligodendrocyte),nostrin(endothelial),tyrobp(microglia),slc1a3
FeaturePlot(object = N44, features = c("OLIG1","OLIG2","GFAP","OPALIN","NOSTRIN","TYROBP","SLC1A3"), reduction= "tsne")## Warning in FetchData(object = object, vars = c(dims, "ident", features), : The
## following requested variables were not found: OLIG1, OPALIN, NOSTRIN, TYROBP
split off neuronal cells (2,11,13,14,12), that are not granule cells (05,0,7,17) or purkinje cells (16), crap 6
neurons<-subset(x=N44,idents=c(2,11,13,14,12,0,5,7,17,16,6),invert=T)
DimPlot(neurons)
neurons <- FindVariableFeatures(object = neurons,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
neurons <- ScaleData(object = neurons,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrixsource('../helperfunctions/pc_modification_functions_S3_forRNA.R')## Loading required package: qlcMatrix## Loading required package: Matrix## Loading required package: slam## Loading required package: sparsesvdneurons<-RunTruncatedPCA(neurons,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: ENSGALG00000047741, GRIA4, GRIA3, GRID2, CACNA2D3, ZNF804B, CERS6, ENSGALG00000009877, ENSGALG00000049672, ADARB2
## NOC4L, IFT122, ENSGALG00000049165, FGF13, ST8SIA1, ENSGALG00000033919, ENSGALG00000047891, EML1, SGCZ, CELF2
## KCNT2, ENSGALG00000052696, ENSGALG00000037747, ASTN2, UNC5C, SGCD, SEMA6D, ENSGALG00000047235, MPZL1, ADCY8
## Negative: CDH19, NINJ2, APOH, ENSGALG00000009355, CNP, BCAS1, ENSGALG00000033591, PLP1, SELENOP2, ST18
## ERMN, ENSGALG00000004734, TMEM88B, NDRG1, ACSS3, CRYAB, CREB5, ZEB2, GLUL, ENSGALG00000027159
## ENSGALG00000052543, AIF1L, HMOX1, ENSGALG00000045795, TMEM125, SALL1, GPR62, PLLP, PAQR7, LY6E
## PC_ 2
## Positive: NEFL, NEFM, COX3, ND5, CYTB, ND4, ENSGALG00000043598, MT-CO1, MT-ND2, COII
## ENSGALG00000036956, GABRA1, DHCR24, CKB, ATP6, ND1, TUBB6, SKP1, CHGB, CTSB
## ENSGALG00000051253, NUB1, S100B, TMED7, CAPN11, PSMD2, YARS, LIN9, VPS29, CLTA
## Negative: MAGI2, NKAIN2, MAP7, QKI, HECW1, SLC44A5, WNK1, DLG1, TCF12, ENSGALG00000012177
## GAB1, MAGI1, SLC12A2, DLC1, PHLPP1, GULP1, ENSGALG00000036916, USP54, ZDHHC14, MARCH8
## SGCZ, ENSGALG00000047741, PRKCA, DAAM2, ENSGALG00000010718, SORCS2, ENSGALG00000009877, ENSGALG00000027159, ITGB8, ENSGALG00000006851
## PC_ 3
## Positive: PAX2, GAD1, KCNC2, ENSGALG00000052279, ZFPM2, PTPRK, PTPRZ1, UNC5C, SLC6A1, ENSGALG00000002671
## ZNF385B, PTN, PDGFD, MEGF11, DGKH, GRM7, CXCL14, CELF2, ENSGALG00000047629, NXPH1
## ENSGALG00000052249, TLL2, COL13A1, GRM2, ENSGALG00000049441, ENSGALG00000047396, ENSGALG00000053609, MMEL1, FGF13, ARHGAP28
## Negative: CCDC85A, ERBB4, CHGB, GABRA1, TSHZ2, NEFM, NOC4L, KCNH8, NEFL, ENSGALG00000049055
## KCNG2, CACNA2D3, GABRG3, RAP1GAP2, TGFB2, DAAM2, DLC1, EIF2B5, ADARB2, TTC31
## SLC35F3, IGSF21, SH3BP4, SH3GLB2, DAB2IP, ENSGALG00000032359, PRRG1, ENSGALG00000030195, ENSGALG00000048955, ENSGALG00000052696
## PC_ 4
## Positive: TSHZ2, KCNH8, PLCXD3, ERBB4, THSD7B, TRPM3, CBLN1, GRIK1, GULP1, CHGB
## STMN1, PIEZO2, SS2, SCG2, ENSGALG00000041624, RIT2, TFPI, CNR1, ENSGALG00000036916, ENSGALG00000013155
## NPY2R, LHFPL6, SLC6A1, ENSGALG00000052249, TAC1, ITM2C, KIAA1328, PLS3, MDGA1, ENSGALG00000049165
## Negative: GAD1, PAX2, PTPRK, DOCK1, MEGF11, NEFM, PDGFD, ENSGALG00000048048, ENSGALG00000010718, SDK1
## CTNNAL1, CKB, COBL, GRIA4, ZFHX4, GPC3, KCTD8, EPHA3, ENSGALG00000052279, TRHDE
## FGF13, NEFL, ENSGALG00000051780, ENSGALG00000047396, NXPH1, KCNC2, VAV2, UGT8, KLHL9, ENSGALG00000053133
## PC_ 5
## Positive: BCL11B, ZFHX4, ENSGALG00000051780, LRFN2, ENSGALG00000011164, LDB2, SYT10, RXFP1, CDH20, SDK1
## PTPRZ1, MAGI2, ARHGAP42, MAP7, ENSGALG00000049165, DLG1, SGCZ, ENPP2, LIN7A, CTNNAL1
## NKAIN2, PDE1C, STAC, DLC1, ENSGALG00000049672, ENSGALG00000006851, ENSGALG00000050791, PDZD2, DEF6, MYLK
## Negative: COL13A1, ANGPT1, ENSGALG00000029012, ENSGALG00000052279, TLL2, ZFPM2, ARHGAP28, GRM2, ENSGALG00000051380, COL12A1
## PLCXD3, ENSGALG00000053609, NOX3, ENSGALG00000002671, CXCL14, SGCD, ENSGALG00000049441, PTN, DGKH, ENSGALG00000046054
## TLL1, ENSGALG00000049789, TRHDE, SEMA3A, ENSGALG00000047629, MEGF11, TOM1L2, TMEM47, CDH13, ENSGALG00000051360ElbowPlot(object = neurons,ndims = 30)
neurons <- JackStraw(object = neurons, dims=30)
neurons<- ScoreJackStraw(neurons,dims=1:30)
JackStrawPlot(object = neurons,dims=1:30)
neurons<- FindNeighbors(neurons,dims=1:23)## Computing nearest neighbor graph## Computing SNNneurons <- FindClusters(object = neurons, resolution = 1)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 1485
## Number of edges: 53152
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.8372
## Number of communities: 14
## Elapsed time: 0 secondsneurons <- RunTSNE(object = neurons, dims = 1:23, perplexity=30, dim.embed = 2)
p1<-DimPlot(object = neurons, reduction = 'tsne', label=TRUE)
p2<-DimPlot(neurons,group.by = 'donor')
p4<-DimPlot(neurons,group.by = 'orig.ident')
(p1 | p2 | p4)
FeaturePlot(object = neurons, features = c("SNAP25","SLC17A6","SLC6A5","GAD1","TBR1","KLHL1"), reduction= "tsne")
FeaturePlot(object = neurons, features = c("SNAP25","SLC17A6","SLC6A5","GAD1","CREB5","GRM2"), reduction= "tsne")
look at contribution from each sort
table(neurons$donor,neurons$RNA_snn_res.1)##
## 0 1 2 3 4 5 6 7 8 9 10 11 12 13
## Chicken3 57 56 14 15 36 19 22 7 6 27 3 4 8 12
## Chicken4 60 87 24 35 37 29 40 14 3 7 9 9 6 7
## Chicken5 162 103 107 81 56 79 49 89 52 2 22 16 13 1VlnPlot(object = neurons, feature = c("nFeature_RNA", "nCount_RNA"))
FeaturePlot(object = neurons, features = c("OLIG1","OLIG2","GFAP","OPALIN","NOSTRIN","TYROBP","SLC1A3"), reduction= "tsne")## Warning in FetchData(object = object, vars = c(dims, "ident", features), : The
## following requested variables were not found: OLIG1, OPALIN, NOSTRIN, TYROBP
remove crappy clusters (9) and the CREB5 positive neuron/non-neuronal cluster (9): creb5 was not detected in starmap.. GRM2 labels granule cell interneurons(compare CBINH2 from mousebrain.org), let’s also kick out 13, which is also strangely overrepresented in mouse 3
neurons2<-subset(neurons, idents=c(9,6,13),invert=T)neurons2 <- FindVariableFeatures(object = neurons2,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
neurons2 <- ScaleData(object = neurons2,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrixneurons2<-RunTruncatedPCA(neurons2,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: ENSGALG00000047741, GRIA3, ENSGALG00000016217, HCN1, MAGI1, FRMPD4, MMP16, CACNA2D3, ENSGALG00000048868, SGCZ
## NFAT5, KLHL32, MAN2A1, QKI, ENSGALG00000037747, ZNF804B, ARID1B, FARP1, ENSGALG00000049496, CPLANE1
## PCNX1, ENSGALG00000036916, ENSGALG00000049672, ZNF804A, IFT122, TENM3, KCNT2, ST8SIA1, ENSGALG00000046863, EBF1
## Negative: COX3, ENSGALG00000043598, ND4, COII, ND5, CYTB, NEFL, MT-CO1, ENSGALG00000036956, MT-ND2
## ND1, ATP6, LDHB, TUBB3, CKB, DHCR24, NEFM, TUBB6, GABRA1, PINK1
## ENSGALG00000050838, CHGB, KCNAB1, ARHGAP19, LAMP5, TNIP1, NUB1, AKR7L, C4orf48, CCNG1
## PC_ 2
## Positive: CHGB, KCNJ6, CCDC85A, ENSGALG00000006864, KCNH8, MAF, AGRN, SNTG2, ENSGALG00000049055, CRHR2
## NOC4L, ENSGALG00000047213, THSD7B, KCNG2, LHFPL6, ENSGALG00000054301, TENM3, LAMP5, TLL1, ZNF804A
## ENSGALG00000052870, GABRA3, ENSGALG00000051945, ADARB2, GABRG3, GABRA1, ENSGALG00000048955, LTBP2, ENSGALG00000049165, KCNAB1
## Negative: GAD1, PAX2, PTPRK, OPRM1, ENSGALG00000052279, KCNC2, UNC5C, GRIK3, ENSGALG00000049321, NXPH1
## HGF, ZFPM2, TFAP2B, ENSGALG00000052669, MEGF11, COBL, PTPRZ1, HTR1B, ENSGALG00000002671, PDGFD
## TOX3, NELL1, GRM7, FGF13, PTN, ENSGALG00000047396, BCL11A, ENSGALG00000053133, TRPC4, PRR35
## PC_ 3
## Positive: NEFM, NEFL, ENSGALG00000048048, CKB, GABRA1, ARHGAP19, ENSGALG00000046863, SDK1, RORB, IGSF9B
## HYDIN, ENSGALG00000051980, NETO1, GRAMD1B, KCNAB1, MAF, ENSGALG00000052696, ENSGALG00000049672, GBA2, VAV2
## PTPRK, ZFHX4, EIF2B5, RASGRF2, ENSGALG00000052264, SRGAP3, EBF1, ENSGALG00000051780, MYH11, CACNA2D3
## Negative: RAB3B, SCG2, FRMPD4, FXYD6, TRPM3, SYP, SCN3B, SS2, GULP1, RIT2
## NPTX2, CDH13, CNR1, PIEZO2, LTBP2, GRIK1, GRIA1, THSD7B, ANGPT1, CXCL14
## SEMA5A, ENSGALG00000041622, ENSGALG00000036916, ITGA9, ENSGALG00000049760, GLRA2, KCNJ6, KCNF1, CAMK2A, EPS8
## PC_ 4
## Positive: BCL11B, BCL11A, LDB2, ZFHX4, ENSGALG00000051780, PTPRZ1, RXFP1, SYT10, ENSGALG00000051186, ENSGALG00000053372
## PTPRT, ENSGALG00000050122, KCNIP1, SGCZ, DACH2, ENSGALG00000049165, ENSGALG00000052264, NETO1, ARHGAP42, SLC9A4
## RORB, ST6GAL2, NPY5R, TRPC4, EPHA7, SDK1, CADPS2, ENSGALG00000042484, CDH20, STAC
## Negative: ZFPM2, ENSGALG00000052279, HGF, LSAMP, RET, TRHDE, ENSGALG00000002671, PTN, MEGF11, GRM3
## PRR35, TFAP2B, ENSGALG00000047396, ENSGALG00000050978, ENSGALG00000053133, ENSGALG00000042339, SGCD, SORCS2, RASGRF2, ANTXR2
## GSG1L, GHR, GRIA1, SORCS3, ENSGALG00000052669, GFRA1, ENSGALG00000051809, ZNF423, DGKH, KCTD8
## PC_ 5
## Positive: NOX3, VEGFD, EDNRA, ZEB2, CADPS2, CDH13, ENSGALG00000048803, DOC2B, TNC, SEMA3E
## PTPRT, PTPRM, COL12A1, TLL2, ENSGALG00000029012, TUBB3, KCNF1, CAMK2A, CYTB, TFAP2B
## CDH4, PLXDC2, ATP2A3, ANGPT1, ENSGALG00000053372, ND4, MT-CO1, LRIG3, MEGF10, SEMA3A
## Negative: GRM3, GABRA1, GSG1L, ZNF804A, HGF, ENSGALG00000047396, MEGF11, TRHDE, COBL, ENSGALG00000051809
## ENSGALG00000047835, ENSGALG00000049978, ENSGALG00000050978, ADARB2, ENSGALG00000046553, PRR35, GLYCTK, RET, RBP1, CACNA2D3
## SLC22A16, ENSGALG00000047853, ZNF804B, MAN2A1, RASGRF2, ENSGALG00000049321, PTN, ZNF423, KCNQ5, MAGI1ElbowPlot(object = neurons2,ndims = 30)
neurons2 <- JackStraw(object = neurons2, dims=30)
neurons2<- ScoreJackStraw(neurons2,dims=1:30)
JackStrawPlot(object = neurons2,dims=1:30)
neurons2<- FindNeighbors(neurons2,dims=1:18)## Computing nearest neighbor graph## Computing SNNneurons2 <- FindClusters(object = neurons2, resolution = 1)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 1318
## Number of edges: 42884
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.8274
## Number of communities: 14
## Elapsed time: 0 secondsneurons2 <- RunTSNE(object = neurons2, dims = 1:18, perplexity=30, dim.embed = 2)
p1<-DimPlot(object = neurons2, reduction = 'tsne', label=TRUE)
p2<-DimPlot(neurons2,group.by = 'donor')
p4<-DimPlot(neurons2,group.by = 'orig.ident')
(p1 | p2 | p4)
FeaturePlot(object = neurons2, features = c("SNAP25","SLC17A6","SLC6A5","GAD1","CREB5","GRM2"), reduction= "tsne")
FeaturePlot(object = neurons2, features = c("SNAP25","SLC17A6","SLC6A5","GAD1","LGI2","EOMES","NXPH1","PTPRK"), reduction= "tsne")
table(neurons2$donor,neurons2$RNA_snn_res.1)##
## 0 1 2 3 4 5 6 7 8 9 10 11 12 13
## Chicken3 56 35 14 13 36 7 19 9 9 12 2 4 8 1
## Chicken4 77 42 25 34 36 12 26 9 9 13 9 10 6 5
## Chicken5 83 82 111 81 54 91 64 68 51 28 23 18 13 13VlnPlot(object = neurons2, feature = c("nFeature_RNA", "nCount_RNA"))
Let’s look at inhibitory neurons.
grab all inhibitory clusters and run through standard steps to improve resolution before filtering out contaminating cell types.
inh<-subset(neurons2,idents=c(4,11,12,10,6,2),invert=F)inh <- FindVariableFeatures(object = inh,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
inh <- ScaleData(object = inh,vars.to.regress = c('nCount_RNA'))## Regressing out nCount_RNA## Centering and scaling data matrixinh<-RunTruncatedPCA(inh,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: ENSGALG00000052279, ZFPM2, NFIB, GRIN3A, NFIA, LSAMP, ZNF521, ZNF536, RORA, ZNF804B
## ENSGALG00000033770, ENSGALG00000002671, ADGRB1, GRM3, DGKH, ACVR2B, TRHDE, DPP6, ENSGALG00000047741, TMEFF2
## SEMA6D, NELL1, LINGO2, ZNF423, HCN1, ZNF804A, KCTD8, FAT3, ENSGALG00000032452, SGCD
## Negative: NEFL, COX3, ENSGALG00000043598, ND4, CYTB, THY1, ND5, ATP6, NEFM, ENSGALG00000036956
## CKB, COII, MT-ND2, ND1, MT-CO1, OPTC, LDHB, CHGB, LAMP5, TAFA1
## ARHGAP19, SKP1, LBH, CALB2, KCNAB1, GABRA1, ENSGALG00000048734, CCNG1, PRDX6, ENSGALG00000050838
## PC_ 2
## Positive: TAFA1, CPNE4, PREX1, ENSGALG00000052781, RORB, PTPRZ1, DACH2, CNTN5, ENSGALG00000051780, ENSGALG00000046863
## LRFN2, ENSGALG00000051172, NETO1, ARHGAP42, EPHA7, ENSGALG00000044478, SGCZ, PLEKHG1, ENSGALG00000028360, SDK1
## ENSGALG00000042484, CNTN4, ENSGALG00000053372, CDH20, ENSGALG00000049165, PLEKHA5, RXFP1, ADGRL3, ENSGALG00000053853, ENSGALG00000049672
## Negative: ZFPM2, GRIN3A, ENSGALG00000052279, PTN, TFAP2B, NFIB, ENSGALG00000033770, GRIA1, ENSGALG00000002671, SORCS3
## TRHDE, SGCD, NCALD, ACVR2B, ANGPT1, DGKH, GULP1, GRM3, GHR, MBP
## ENSGALG00000050732, SORCS2, ENSGALG00000053133, ENSGALG00000042339, MEGF11, PRR35, CACNG5, ZNF423, LSAMP, COX3
## PC_ 3
## Positive: PTPRT, CDH13, NOX3, ZEB2, ENSGALG00000053372, GRIK1, KCNF1, SLC6A1, ENSGALG00000052249, VEGFD
## CADPS2, CAMK2A, DOC2B, ANGPT1, PTPRM, LMO7, PLXDC2, TENM3, PTPRZ1, ENSGALG00000038930
## LRIG3, FXYD6, ENSGALG00000048972, PDE3A, KCNT2, SYP, CDH4, ENSGALG00000052870, CNTN5, SEMA5A
## Negative: GABRA1, SNTG1, GRM3, NEFM, CKB, MEGF11, ENSGALG00000053975, EPB41L1, NEFL, PLEKHG1
## LAMP5, ENSGALG00000047396, ENSGALG00000052781, TRHDE, ANOS1, ENSGALG00000048048, RASGRF2, GSG1L, CACNA2D3, PAPPA
## TUBB6, ZNF804A, GPLD1, SLC22A16, IQGAP2, ATP10B, ENSGALG00000053282, ARHGAP19, SCNN1B, KCTD8
## PC_ 4
## Positive: FAM135B, SYT10, ENSGALG00000046553, CNR1, LINGO2, ENSGALG00000028360, LRFN2, SGCZ, LNX1, RORA
## ENSGALG00000049876, GRIA3, ENSGALG00000048972, ENSGALG00000049978, MMP16, ENSGALG00000038930, PTPRZ1, ENSGALG00000054072, FRMPD4, NTS
## STAC, ENSGALG00000032452, WNT7B, ENSGALG00000054369, ENSGALG00000048804, ENSGALG00000051831, ADGRL3, ADAMTS9, ENSGALG00000048886, ADARB2
## Negative: THY1, NEFL, NEFM, KIF1A, ENSGALG00000040371, CYTB, ND1, MT-ND2, ATP6, ARHGAP19
## ND5, COII, CKB, AARS, NDRG3, LDHB, ND4, VPS29, COX3, GRIN2A
## TM9SF3, MT-CO1, LAMP5, ENSGALG00000048350, ENSGALG00000036956, SEMA3A, LUZP2, ENSGALG00000043598, ACTR2, SKP1
## PC_ 5
## Positive: KCNB2, RIT2, ENSGALG00000006851, NELL1, GLRA3, CRHR2, SYT10, MYLK, SHISAL1, FAM135B
## SVEP1, ITM2C, THY1, CHRNA3, ENSGALG00000048972, SCN3B, RXFP1, GABRA1, FRMD3, GREB1L
## CACNA1E, EBF2, GABRG3, SCN5A, ST3GAL1, ENSGALG00000047544, ENSGALG00000038930, ENSGALG00000049165, NTS, LRFN2
## Negative: NOX3, VEGFD, KCNF1, LRIG3, EDNRA, COL12A1, CACNA1H, ENSGALG00000052870, EPHA3, TMCO4
## GABRA3, LMO7, SEMA3A, ENSGALG00000036204, FAT3, GRIN2A, LSAMP, PLCG2, PLA2G2E, UBXN10
## GRIK1, LUZP2, ENSGALG00000052988, CHST1, ENSGALG00000053608, CHGB, GRID2, ENSGALG00000047741, PDE3A, CAMK2AElbowPlot(object = inh)
#inh <- JackStraw(object = inh, dims=20)
#inh <- ScoreJackStraw(inh,dims=1:20)
#JackStrawPlot(object = inh,dims=1:20)inh<- FindNeighbors(inh,dims=1:9)## Computing nearest neighbor graph## Computing SNNinh <- FindClusters(object = inh, resolution = 2)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 478
## Number of edges: 12661
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.6433
## Number of communities: 10
## Elapsed time: 0 secondsinh <- RunTSNE(object = inh, dims = 1:9, perplexity=30, dim.embed = 2)
p1<-DimPlot(object = inh, reduction = 'tsne', label=TRUE)
p2<-DimPlot(inh,group.by = 'donor')
p4<-DimPlot(inh,group.by = 'orig.ident')
plot_grid(p1,p2,p4,ncol=2)
FeaturePlot(inh,c("nFeature_RNA","nCount_RNA"))
merge clusters using allen package.
rd.dat <- t(GetAssayData(object = inh, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 40)
merge.result <- merge_cl(as.matrix(GetAssayData(object = inh, slot = "data")),
cl = inh$RNA_snn_res.2,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{inh$merged.res.2<-1
} else {
inh$merged.res.2<-paste0("inh_",merge.result$cl)
}p2<-DimPlot(inh,group.by = "merged.res.2",label=T)
p1<-DimPlot(inh,group.by = "RNA_snn_res.2")
p4<-DimPlot(inh,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
FeaturePlot(inh,c("SNAP25","SLC17A6","GAD1","SLC6A5","ETV1","GAD2"))
Idents(inh)<-'merged.res.2'
VlnPlot(inh,c("nCount_RNA","nFeature_RNA"))
m1v4<-FindMarkers(inh,ident.1 = "inh_1",ident.2 = "inh_4",logfc.threshold = 1,min.diff.pct = 0.3)
m1v4## p_val avg_logFC pct.1 pct.2 p_val_adj
## CHL1 3.186402e-16 -2.726492 0.293 0.923 7.760800e-12
## CPNE4 2.711836e-14 2.474431 0.960 0.577 6.604947e-10
## SEMA5A 1.583002e-13 -3.606621 0.587 0.904 3.855561e-09
## RXFP1 3.924247e-13 2.077871 0.947 0.462 9.557896e-09
## CDH8 3.819177e-11 -1.548820 0.587 0.962 9.301987e-07
## PDGFD 1.003454e-10 -2.221777 0.573 0.904 2.444012e-06
## LINGO2 3.929679e-10 -1.657781 0.520 0.904 9.571126e-06
## FAT3 7.751255e-10 3.508294 0.800 0.442 1.887896e-05
## CRHR2 1.134364e-09 -2.141629 0.333 0.769 2.762857e-05
## CALB2 4.092796e-09 -2.139505 0.280 0.731 9.968413e-05
## PTPRM 4.475197e-09 -2.502299 0.480 0.808 1.089979e-04
## NXPH1 1.031211e-08 -1.137233 0.613 0.923 2.511617e-04
## DOK5 1.480961e-08 4.120689 0.467 0.000 3.607028e-04
## NTS 4.740685e-08 -2.009616 0.347 0.750 1.154641e-03
## CASZ1 7.921160e-08 1.908021 0.867 0.538 1.929278e-03
## BANK1 1.603279e-07 -2.060108 0.413 0.788 3.904947e-03
## ENSGALG00000048913 1.879298e-07 -2.201271 0.093 0.500 4.577218e-03
## ENSGALG00000051186 1.894273e-07 1.142994 0.840 0.442 4.613691e-03
## VWC2 2.897079e-07 -1.241635 0.173 0.615 7.056126e-03
## ENSGALG00000049321 3.883602e-07 1.924857 0.707 0.308 9.458902e-03
## PAX5 4.591861e-07 -4.201352 0.267 0.615 1.118394e-02
## NDST4 6.594406e-07 1.322652 0.827 0.500 1.606134e-02
## ENSGALG00000050067 8.719248e-07 1.691177 0.640 0.212 2.123660e-02
## ZNF423 8.724931e-07 -1.045110 0.600 0.923 2.125044e-02
## PREX2 2.250262e-06 1.556981 0.867 0.519 5.480737e-02
## SPOCK1 2.392654e-06 -1.409158 0.467 0.769 5.827548e-02
## HTR1B 2.788743e-06 1.514502 0.667 0.269 6.792263e-02
## BMP5 3.001596e-06 -2.711121 0.280 0.654 7.310687e-02
## KCNIP1 3.023249e-06 1.198556 0.840 0.519 7.363424e-02
## ENSGALG00000050348 3.184800e-06 -4.347858 0.053 0.365 7.756898e-02
## PLXNB2 3.757078e-06 -2.955594 0.173 0.500 9.150740e-02
## ENSGALG00000055031 8.545512e-06 2.367297 0.400 0.058 2.081345e-01
## ZEB2 1.028315e-05 -1.895874 0.253 0.615 2.504563e-01
## FAM184B 1.154136e-05 -1.729730 0.293 0.635 2.811013e-01
## CAPN5 1.157627e-05 1.140385 0.773 0.423 2.819516e-01
## ATP6V1C2 1.176743e-05 -1.824948 0.133 0.462 2.866076e-01
## ENSGALG00000050122 1.846363e-05 1.763743 0.720 0.404 4.497002e-01
## ENSGALG00000052669 2.249838e-05 -1.651319 0.427 0.731 5.479707e-01
## IGF-I 4.117168e-05 1.053231 0.627 0.269 1.000000e+00
## PTPRO 5.158501e-05 -1.036188 0.427 0.731 1.000000e+00
## ENSGALG00000051715 5.494039e-05 1.888470 0.480 0.154 1.000000e+00
## PAK1 6.210215e-05 2.048749 0.480 0.154 1.000000e+00
## VIM 1.304170e-04 1.006052 0.653 0.308 1.000000e+00
## NDNF 1.373117e-04 1.023229 0.773 0.442 1.000000e+00
## ENSGALG00000052197 1.776463e-04 2.108139 0.400 0.096 1.000000e+00
## MEI4 1.879074e-04 1.469277 0.680 0.365 1.000000e+00
## STK32A 1.909652e-04 1.046798 0.507 0.173 1.000000e+00
## ENSGALG00000052017 2.488339e-04 2.035706 0.440 0.135 1.000000e+00FeaturePlot(inh,c("CHL1","CPNE4","SEMA5A","RXFP1"))
inhmarkers<-FindAllMarkers(inh,logfc.threshold = 1,min.diff.pct = 0.3)## Calculating cluster inh_3## Calculating cluster inh_6## Calculating cluster inh_0## Calculating cluster inh_9## Calculating cluster inh_1## Calculating cluster inh_8## Calculating cluster inh_4inhmarkers %>% group_by(cluster) %>% top_n(10,avg_logFC)## # A tibble: 70 x 7
## # Groups: cluster [7]
## p_val avg_logFC pct.1 pct.2 p_val_adj cluster gene
## <dbl> <dbl> <dbl> <dbl> <dbl> <fct> <chr>
## 1 2.04e-61 4.02 0.698 0.017 4.97e-57 inh_3 ENSGALG00000004653
## 2 4.98e-57 3.19 0.992 0.574 1.21e-52 inh_3 MEGF11
## 3 5.56e-55 2.88 1 0.659 1.35e-50 inh_3 TRHDE
## 4 1.20e-37 2.90 0.77 0.213 2.93e-33 inh_3 ENSGALG00000047396
## 5 1.04e-36 3.82 0.476 0.023 2.52e-32 inh_3 ENSGALG00000051809
## 6 4.85e-32 3.89 0.683 0.219 1.18e-27 inh_3 ENSGALG00000050978
## 7 1.03e-27 2.85 0.444 0.045 2.51e-23 inh_3 CACNG5
## 8 6.17e-25 2.91 0.452 0.068 1.50e-20 inh_3 ENSGALG00000051340
## 9 1.01e-21 3.38 0.373 0.048 2.46e-17 inh_3 OPN3
## 10 7.15e-17 2.67 0.69 0.355 1.74e-12 inh_3 CDH23
## # ... with 60 more rowsDimPlot(inh,group.by = 'donor')
FeaturePlot(inh,c("SNAP25","SLC17A6","GAD1","OLIG1","OLIG2","GFAP","OPALIN","NOSTRIN","TYROBP","SLC1A3","CALB1","PCP1","PCP4","GRM1"))## Warning in FetchData(object = object, vars = c(dims, "ident", features), : The
## following requested variables were not found: OLIG1, OPALIN, NOSTRIN, TYROBP,
## PCP1
VlnPlot(inh,c("GRM1"))
all mouse CN clusters are GRM1+. all chicken ex clusters are GRM1+. all but inh_9 and inh_6 are GRM1+. these are likely contaminants to remove. run cleaned up dataset through pipeline.
inh<-subset(inh,idents=c("inh_6","inh_9"),invert=T)inh <- FindVariableFeatures(object = inh,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
inh <- ScaleData(object = inh,vars.to.regress = c('nCount_RNA'))## Regressing out nCount_RNA## Centering and scaling data matrix#inh <- RunPCA(object = inh,npcs = 30,verbose = FALSE)inh<-RunTruncatedPCA(inh,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: TAFA1, ENSGALG00000053372, PTPRZ1, CNTN5, PREX1, PTPRT, CPNE4, LRFN2, ENSGALG00000049165, SGCZ
## EPHA7, RORB, SYT10, RXFP1, ENSGALG00000028360, ENSGALG00000051780, KCNIP1, DACH2, EBF2, CADPS2
## ARHGAP42, ENSGALG00000044478, SDK1, NETO1, ENSGALG00000054301, ENSGALG00000051172, ENSGALG00000042484, STAC, NPY5R, ST6GAL2
## Negative: ZFPM2, ENSGALG00000052279, NFIB, GRIN3A, GRM3, TRHDE, ENSGALG00000033770, PTN, LSAMP, MEGF11
## ENSGALG00000002671, ZNF423, NELL1, ENSGALG00000042339, GSG1L, DGKH, KCNMA1, ENSGALG00000053133, NCALD, LINGO2
## NFIA, ZNF521, ENSGALG00000047396, KCTD8, ZNF804B, SORCS2, GRIA1, ZNF536, ZNF804A, TMEFF2
## PC_ 2
## Positive: SCN3B, ENSGALG00000048972, GLRA3, SLC6A1, ZEB2, RIT2, PGAM1, ENSGALG00000052249, ENSGALG00000038930, SEMA5A
## ENSGALG00000053013, COX3, ENSGALG00000047544, SYT10, IL1RAPL2, ITM2C, PTPRT, SORCS3, MT-CO1, CDH13
## KCNB2, ENSGALG00000050732, ENSGALG00000052771, SVEP1, MYLK, TLL1, MBP, NTS, COII, MT-ND2
## Negative: ENSGALG00000052781, PLEKHG1, ENSGALG00000048048, ENSGALG00000046863, SNTG1, EPB41L1, SLCO4A1, ENSGALG00000049672, SLC2A5, ENSGALG00000047741
## CACNA2D3, HYDIN, PPP4R4, ANOS1, PROSER1, ENSGALG00000052696, GPLD1, ENSGALG00000053975, UBASH3B, TEAD4
## ENSGALG00000051172, SACS, CDH20, ANKS1A, ENSGALG00000054318, ENSGALG00000049915, ENSGALG00000011356, ARHGAP42, SF3B1, ENSGALG00000051780
## PC_ 3
## Positive: SHISAL1, ENSGALG00000048482, LDHB, ENSGALG00000048350, ENSGALG00000040371, ATP6, ANGPT1, PGAM1, NDRG3, MT-ND2
## ENSGALG00000006851, ATP2A2, ND1, CYTB, ND5, ENSGALG00000050732, ATP6V0D1, PSAP, KIF1A, RAB2A
## VPS29, EBF2, MT-CO1, COX3, MTMR9, RAB5C, PTPRM, RIT2, ZEB2, TRAPPC2
## Negative: ENSGALG00000049978, ENSGALG00000054072, EPB41L1, JCAD, CNR1, ZNF804A, ENSGALG00000054369, ENSGALG00000048877, ENSGALG00000046553, CBX2
## IGF2BP2, CACNG5, ENSGALG00000051424, FAM110B, ENSGALG00000051086, ENSGALG00000047870, FAT3, GRIA3, TMED6, ENSGALG00000013624
## MARCHF2, NWD2, ENSGALG00000049789, ENSGALG00000033919, HCN1, GPLD1, CEBPB, SGCZ, ENSGALG00000054745, DPP6
## PC_ 4
## Positive: CYTB, COX3, COII, ENSGALG00000036956, ND4, MT-ND2, ATP6, ND5, ENSGALG00000043598, ND1
## MT-CO1, LDHB, ENSGALG00000050838, GABRA1, PRDX6, CACNA2D3, AARS, PGAM1, CHGB, ATP6V0D1
## EIF3L, HMOX2, COL12A1, HDHD2, MEGF11, SPARC, UBE2V1, C1QTNF4, SLC1A3, OPRK1
## Negative: CRHR2, ZEB2, ENSGALG00000050732, ENSGALG00000006851, ENSGALG00000048972, ST3GAL1, TLL1, ENSGALG00000038930, ENSGALG00000047544, MMP16
## GLRA2, PLEKHA5, GLRA3, ENSGALG00000052771, RIT2, GRIA3, ANGPT1, KCNB2, PDE5A, SLC22A15
## KCNT2, ENSGALG00000028360, ENSGALG00000015418, FRMPD4, ENSGALG00000036916, GREB1L, CHRNA3, ADGRL3, ME1, ENSGALG00000049116
## PC_ 5
## Positive: TLL1, ENSGALG00000047544, ADARB2, ENSGALG00000031929, ENSGALG00000052771, PDGFD, ENSGALG00000050732, GLRA2, CDH20, ST3GAL1
## SVEP1, TOX, ERG, ZEB2, ENSGALG00000046863, ENSGALG00000053965, CHRNA3, CRHR2, ENSGALG00000054359, ENSGALG00000052755
## ENSGALG00000046944, OPTC, RALYL, PDE5A, ENSGALG00000051780, SST, GRIK1, GLRA3, ATP6V1C2, CACNA2D1
## Negative: RXFP1, FAT1, SYT10, ENSGALG00000049165, GABRG3, ENSGALG00000053282, ENSGALG00000030006, ADGRB1, MYO7A, GSG1L
## TNC, PTPRT, CACNA2D3, ENSGALG00000053372, DOC2B, ENSGALG00000028360, ENSGALG00000053133, ENSGALG00000047853, ENSGALG00000049178, IQGAP2
## MYLK, ENSGALG00000033770, ENSGALG00000049116, ZBTB20, PACRG, THSD4, ENSGALG00000048356, FRMPD4, ENSGALG00000048311, ENSGALG00000047396ElbowPlot(object = inh)
#inh <- JackStraw(object = inh, dims=20)
#inh <- ScoreJackStraw(inh,dims=1:20)
#JackStrawPlot(object = inh,dims=1:20)inh<- FindNeighbors(inh,dims=1:8)## Computing nearest neighbor graph## Computing SNNinh <- FindClusters(object = inh, resolution = 2)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 417
## Number of edges: 11384
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.5945
## Number of communities: 10
## Elapsed time: 0 secondsinh <- RunTSNE(object = inh, dims = 1:9, perplexity=30, dim.embed = 2)
p1<-DimPlot(object = inh, reduction = 'tsne', label=TRUE)
p2<-DimPlot(inh,group.by = 'donor')
p4<-DimPlot(inh,group.by = 'orig.ident')
(p1|p2|p4)
merge clusters using allen package.
rd.dat <- t(GetAssayData(object = inh, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 40)
merge.result <- merge_cl(as.matrix(GetAssayData(object = inh, slot = "data")),
cl = inh$RNA_snn_res.2,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{inh$merged.res.2<-1
} else {
inh$merged.res.2<-paste0("inh_",merge.result$cl)
}p2<-DimPlot(inh,group.by = "merged.res.2",label=T)
p1<-DimPlot(inh,group.by = "RNA_snn_res.2")
p4<-DimPlot(inh,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
Let’s look at excitatory neurons. select all excitatory neurons and run through standard pipeline.
ex<-subset(neurons2,idents=c(7,1,0,8,9,5,3))ex <- FindVariableFeatures(object = ex,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
ex <- ScaleData(object = ex,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrixex<-RunTruncatedPCA(ex,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: PDE10A, CACNA2D3, ZNF804A, MMP16, WNK1, ENSGALG00000049496, ENSGALG00000048868, ENSGALG00000049165, KMT2C, CBLB
## SPIRE1, TRPS1, SUGCT, ENSGALG00000037747, NFAT5, PPP1R14C, CDH8, ENSGALG00000049672, THSD7B, ENSGALG00000046863
## ENSGALG00000029316, NOC4L, ENSGALG00000054301, MSH3, PTPRR, DACH2, CDH4, UBN2, DENND5B, KCNH8
## Negative: ATP1B1, NEFL, ENSGALG00000045548, COX3, ENSGALG00000043598, ND4, COII, CYTB, ND5, MT-CO1
## ND1, ENSGALG00000036956, CKB, MT-ND2, ATP6, ND3, TUBB3, LDHB, TUBB4B, ATP5A1Z
## NEFM, CHGB, DHCR24, GABRA1, NEFH, TPI1, UBL7, SNCG, S100B, PRDX1
## PC_ 2
## Positive: RAB3B, ENSGALG00000048785, PDE1C, SCG2, SS2, SCN3B, PIEZO2, SYT10, NPTX2, KCNIP1
## TRPM3, LTBP2, FXYD6, CXCL14, CNR1, SRD5A2, SYP, ENSGALG00000041622, OPRM1, RIT2
## GRM7, ENSGALG00000053609, TRPS1, PTPRT, CDH8, CDH13, ENSGALG00000049760, ENSGALG00000052027, PTPRZ1, GRIA1
## Negative: PVALB, MAF, CCDC85A, NEFM, KCNG2, ENSGALG00000048048, NEFL, EIF2B5, GABRA1, IGSF9B
## NOC4L, ENSGALG00000006864, GABRG3, CACNA2D3, ENSGALG00000046863, ARHGAP19, DOCK1, KIF5A, ENSGALG00000048843, GBA2
## ENSGALG00000052954, ENSGALG00000048867, CABP1, FBXO2, TRHDE, PREX2, ENSGALG00000006851, AFF1, NEFH, ENSGALG00000046944
## PC_ 3
## Positive: CHRNA4, ENSGALG00000049055, UTRN, NXPH1, IL1RAPL2, ADAMTSL1, SORCS3, PTN, ENSGALG00000047213, SEMA3A
## PKIB, SEMA5B, CDH20, ZNF423, FAT3, SNTB1, LHFPL2, PREX2, DRD2, GALNT14
## TAC1, STAC, ENSGALG00000049731, ENSGALG00000046918, CALD1, RSPO1, ENSGALG00000053263, ENSGALG00000046961, ENSGALG00000009458, ENSGALG00000050067
## Negative: ADARB2, NPTX2, KCNH8, ENSGALG00000048785, ZNF804A, RUNX2, PIEZO2, LAMP5, SVIL, SS2
## ENSGALG00000049933, ENSGALG00000031713, THSD7B, RAB3B, CNR1, SYT17, ENSGALG00000054211, TOM1L1, MPPED2, GRM7
## LHFPL6, SRD5A2, GRM3, PDE10A, CABP1, TRPS1, SCG2, CRHR2, SMCO4, ENSGALG00000054223
## PC_ 4
## Positive: ADARB2, KCNG2, MAF, CRHR2, ENSGALG00000006864, KCNH8, MPPED2, LHFPL6, VCAN, ENSGALG00000052870
## ENSGALG00000031713, ZNF804A, RBM24, THSD7B, ALDH1A3, CACNA2D3, CNR1, CCDC85A, GABRA1, CLCA1
## SVIL, ENSGALG00000049933, C5orf49, LAMP5, ENSGALG00000050018, RUNX2, ENSGALG00000054301, ENSGALG00000044293, ENSGALG00000050791, METRNL
## Negative: SORCS3, SNTB1, STAC, NXPH1, ENSGALG00000053998, SOX6, ENSGALG00000046961, CHRNA4, SYP, ENSGALG00000047891
## PKIB, MEGF10, ENSGALG00000035051, SEMA3D, CALD1, ENSGALG00000051186, ADAMTSL1, ENSGALG00000046918, SEMA5B, IL1RAPL2
## DACH2, ENSGALG00000047181, LHFPL2, GALNT14, PTPRT, ENSGALG00000036204, UTRN, DPYD, SORCS2, RIT2
## PC_ 5
## Positive: RIT2, GABRG3, STAC, SORCS3, PLXDC2, PKIB, ENSGALG00000053998, ENSGALG00000046961, ENSGALG00000036204, SOX6
## OPRM1, ENSGALG00000047891, NXPH1, PTPRT, CNR1, CDH13, PTN, ENPP2, ENSGALG00000035051, ENSGALG00000029796
## CBLN3, ENSGALG00000050122, MEGF10, ENSGALG00000047181, GRM3, GRIA1, NRP1, PAX5, ENSGALG00000009458, ENSGALG00000051186
## Negative: TAC1, ENSGALG00000049055, BCL11B, SPON1, ENSGALG00000047213, ENSGALG00000053263, UNC5C, RSPO1, UTRN, ANGPT1
## DRD2, SEMA3C, KCNB2, SYT10, KCNF1, TAFA1, ENSGALG00000049165, ENSGALG00000050067, ENSGALG00000049731, ADAMTSL1
## CHGB, ZNF423, GALNT14, CADPS2, PTPRZ1, PCDH10, CADM1, FBXL7, SEMA3E, BPIFB2ElbowPlot(object = ex)
#ex <- JackStraw(object = ex, dims=20)
#ex <- ScoreJackStraw(ex,dims=1:20)
#JackStrawPlot(object = ex,dims=1:20)cluster at low resolution
ex<- FindNeighbors(ex,dims=1:12)## Computing nearest neighbor graph## Computing SNNex <- FindClusters(object = ex, resolution = 1)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 821
## Number of edges: 26642
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.7606
## Number of communities: 9
## Elapsed time: 0 secondsat higher resolution
ex<- FindNeighbors(ex,dims=1:12)## Computing nearest neighbor graph## Computing SNNex <- FindClusters(object = ex, resolution = 3)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 821
## Number of edges: 26642
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.5492
## Number of communities: 16
## Elapsed time: 0 secondsex <- RunTSNE(object = ex, dims = 1:12, perplexity=30, dim.embed = 2,)
p1<-DimPlot(object = ex, reduction = 'tsne', label=TRUE)
p2<-DimPlot(ex,group.by = 'donor')
p4<-DimPlot(ex,group.by = 'orig.ident')
plot_grid(p1,p2,p4,ncol=2)
merge clusters using allen package.
rd.dat <- t(GetAssayData(object = ex, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 40)
merge.result <- merge_cl(as.matrix(GetAssayData(object = ex, slot = "data")),
cl = ex$RNA_snn_res.3,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{ex$merged.res.3<-1
} else {
ex$merged.res.3<-paste0("ex_",merge.result$cl)
}p2<-DimPlot(ex,group.by = "merged.res.3",label=T)
p1<-DimPlot(ex,group.by = "RNA_snn_res.3")
p4<-DimPlot(ex,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
FeaturePlot(ex,c("SNAP25","SLC17A6","TBR1","PAX5","LHX9","RSPO1"))
Idents(ex)<-'merged.res.3'
VlnPlot(ex,c("nCount_RNA","nFeature_RNA"))
subcluster the big groups of excitatory neurons to make sure we are not missing any structure. start with ex_0
ex0<-subset(ex,idents=c("ex_0"))ex0 <- FindVariableFeatures(object = ex0,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
ex0 <- ScaleData(object = ex0,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrixex0<-RunTruncatedPCA(ex0,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: ENSGALG00000046702, ENSGALG00000049761, ENSGALG00000049148, ENSGALG00000051949, DGKB, FGF14, ENSGALG00000050832, MCTP1, IL1RAPL1, GRIK2
## CCNY, CAMK1D, ENSGALG00000047741, HSF1, ADGRB3, TENM2, RALYL, ENSGALG00000050676, FBXL17, XKR4
## NRG2, ENSGALG00000052495, CACNA2D3, RBFOX1, AUTS2, PPARGC1A, DSCAM, TAFA2, FSTL5, LRRC4C
## Negative: RAB18, PSMD5, C1D, PDP1, ENSGALG00000049127, NAPG, CYCS, YWHAZ, HSPA12A, EIF4E
## COPE, TSPAN3, SLC48A1, ERCC3, NDFIP1, RCBTB1, CHCHD4, UBL3, CCNG1, DIPK2A
## RAP1B, ENSGALG00000022875, ENSGALG00000053036, DTD1, CSTB, FNTA, LRP11, BORA, YIPF4, DESI2
## PC_ 2
## Positive: LRRC3B, COII, COX3, ENSGALG00000048485, ENSGALG00000047456, WNT2B, ND1, CYTB, ENSGALG00000053400, CXCL14
## BMP4, ENSGALG00000049244, ENSGALG00000049466, ENSGALG00000050068, ENSGALG00000051181, ND4, GAREM2, FAM83B, ENSGALG00000051881, CDK19
## CD9, PTGS2, POPDC2, ATP6, CD36, ZNF516, GPR63, ENSGALG00000050538, ENSGALG00000048660, ENSGALG00000043598
## Negative: VCP, ARHGAP19, MOB4, PDCD5, MCRIP1, PARK7, IPO5, NDFIP1, CSNK1G1, DHCR24
## SEPTIN9, DNAJC8, ENSGALG00000027334, CHGB, ENSGALG00000011356, ENSGALG00000006056, TPD52, TSPAN3, TRIM37, SDHB
## DNAJA2, AKR7L, ATCAY, RPS6KL1, TTC1, UHMK1, HEATR5B, ENSGALG00000006723, ENSGALG00000007287, EIF2S1
## PC_ 3
## Positive: ENSGALG00000049164, ENSGALG00000054359, ENSGALG00000051209, CDH6, TP63, BBS9, ITPKB, ENSGALG00000002561, ENSGALG00000052155, ENSGALG00000046893
## MTNR1A, ENSGALG00000047437, ENSGALG00000028360, ZMYND12, XDH, ENSGALG00000048913, ENSGALG00000054683, ENSGALG00000053491, OTULIN, ATP8B2
## ENSGALG00000038720, EBF2, SDC2, PAK4, EBF1, FYB2, IL11RA, DHCR24, FSTL5, EPHA5
## Negative: ENSGALG00000051887, ENSGALG00000049302, CCR10, ENSGALG00000029853, HHIP, GPR19, ENSGALG00000048122, KCNA4, ENSGALG00000046774, ENSGALG00000010457
## ENSGALG00000023083, ENSGALG00000039896, DPCD, KIAA1024, ENSGALG00000046927, ENSGALG00000000293, ENSGALG00000047638, ENSGALG00000016570, PDK4, ENSGALG00000050384
## ENSGALG00000013437, ENSGALG00000052885, ENSGALG00000054603, IL5RA, CD2AP, PRLHR, DCLRE1C, PKIB, ENSGALG00000054607, VNN1
## PC_ 4
## Positive: ENSGALG00000054359, ENSGALG00000050914, ENSGALG00000048945, ENSGALG00000027397, ENSGALG00000002561, TMEM220, ENSGALG00000019568, EEF2K, BRINP2, MTNR1A
## ENSGALG00000039896, ENSGALG00000013437, ENSGALG00000052155, ENSGALG00000054607, ENSGALG00000047684, ENSGALG00000049600, ENSGALG00000051887, ENSGALG00000054301, PLAA, ENSGALG00000020921
## THAP9, ENSGALG00000001051, ENSGALG00000028207, TERB1, CCR10, AMY2A, WDR92, DNAL4, AKAP8L, GPR19
## Negative: PLPP3, BAHD1, TMEM100, ENSGALG00000052249, ENSGALG00000048733, OLAH, SOX5, ENSGALG00000005303, ENSGALG00000046548, SDC4
## GFAP, AGT, SH2D4A, CLRN1, QKI, CALML4, MTHFD1L, OPNVA, ENSGALG00000047717, ENSGALG00000026896
## ENSGALG00000047991, DRAM1, RMI1, SLC6A11, MLC1, PLAGL1, ENSGALG00000046829, SLC6A1, ENSGALG00000051031, ALKBH4
## PC_ 5
## Positive: ASCC3, ENSGALG00000048389, ENSGALG00000051819, SLC44A1, WNT2B, GRM8, ENSGALG00000049412, SYT1, IST1, ENSGALG00000048868
## ENSGALG00000031836, ENSGALG00000053400, NOP56, CXCL14, BMP4, ENSGALG00000051181, ENSGALG00000048224, POPDC2, ENSGALG00000053759, LRTM1
## ENSGALG00000048485, BACH2, ENSGALG00000047456, ATP6V1C1, ENSGALG00000041906, CAND1, HOMER1, XK, MYLK4, ENSGALG00000047936
## Negative: ENSGALG00000051031, ENSGALG00000053247, ENSGALG00000047437, SH3PXD2B, ENSGALG00000053491, ITPRID2, ENSGALG00000027397, ENSGALG00000049696, ENSGALG00000040613, SNX22
## FYB2, TIMM21, PDK4, DCLRE1C, AMY2A, ENSGALG00000053035, ENSGALG00000002561, TSPAN14, ENSGALG00000048897, PAQR8
## ENSGALG00000031900, PEX11A, ITPKB, FRRS1L, ENSGALG00000053355, FAM98B, SLC1A3, ENSGALG00000052011, TP63, MOSPD1ElbowPlot(object = ex0)
#ex0 <- JackStraw(object = ex0, dims=20)
#ex0 <- ScoreJackStraw(ex0,dims=1:20)
#JackStrawPlot(object = ex0,dims=1:20)ex0<- FindNeighbors(ex0,dims=1:8)## Computing nearest neighbor graph## Computing SNNex0 <- FindClusters(object = ex0, resolution = 3)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 133
## Number of edges: 4660
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.0714
## Number of communities: 42
## Elapsed time: 0 seconds## 27 singletons identified. 15 final clusters.ex0 <- RunTSNE(object = ex0, dims = 1:10, perplexity=20, dim.embed = 2,)
p1<-DimPlot(object = ex0, reduction = 'tsne', label=TRUE)
p2<-DimPlot(ex0,group.by = 'donor')
p4<-DimPlot(ex0,group.by = 'orig.ident')
plot_grid(p1,p2,p4,ncol=2)
merge clusters using allen package.
rd.dat <- t(GetAssayData(object = ex0, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 40)
merge.result <- merge_cl(as.matrix(GetAssayData(object = ex0, slot = "data")),
cl = ex0$RNA_snn_res.3,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{ex0$merged.res.3<-1
} else {
ex0$merged.res.3<-paste0("ex_",merge.result$cl)
}p2<-DimPlot(ex0,group.by = "merged.res.3",label=T)
p1<-DimPlot(ex0,group.by = "RNA_snn_res.3")
p4<-DimPlot(ex0,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
Idents(ex0)<-'merged.res.3'
m<-FindMarkers(ex0,ident.1 = "ex_0",logfc.threshold = 1)
m## p_val avg_logFC pct.1 pct.2 p_val_adj
## ENSGALG00000050659 1.745463e-23 2.803155 0.958 0.046 4.251248e-19
## ENSGALG00000049605 1.177473e-17 3.360908 0.958 0.165 2.867854e-13
## ENSGALG00000049148 1.177832e-17 3.807814 0.958 0.174 2.868729e-13
## MARK2 1.230923e-16 2.733131 1.000 0.294 2.998037e-12
## ENSGALG00000047133 7.682107e-16 1.956112 0.583 0.009 1.871054e-11
## WSB1 3.484631e-15 2.786582 0.750 0.073 8.487168e-11
## ENSGALG00000036447 4.108313e-15 3.049404 0.667 0.037 1.000621e-10
## CAPN1 9.417636e-15 2.310860 0.958 0.321 2.293759e-10
## DOK6 9.723489e-15 3.541860 0.917 0.202 2.368253e-10
## RELA 1.309989e-14 1.968865 0.917 0.220 3.190609e-10
## ENSGALG00000046702 1.658049e-14 3.722721 1.000 0.413 4.038343e-10
## STIP1 2.426785e-14 2.648681 0.917 0.211 5.910677e-10
## ENSGALG00000049761 1.741299e-13 3.415983 0.958 0.459 4.241109e-09
## ENSGALG00000050982 1.950284e-13 3.314369 0.500 0.009 4.750113e-09
## ENSGALG00000054889 1.060563e-12 1.463405 0.833 0.165 2.583107e-08
## ENSGALG00000050832 1.384007e-12 2.506723 0.875 0.211 3.370886e-08
## ENSGALG00000049082 2.142011e-12 2.444717 0.792 0.147 5.217082e-08
## OGT 2.450091e-12 2.448643 1.000 0.679 5.967442e-08
## FUS 2.910748e-12 2.442617 0.708 0.101 7.089419e-08
## ENSGALG00000054443 4.012738e-12 2.412272 1.000 0.339 9.773425e-08
## ENSGALG00000050720 6.132920e-12 2.906771 0.625 0.064 1.493734e-07
## ENSGALG00000051949 1.047422e-11 3.717598 0.833 0.211 2.551101e-07
## CCNY 1.105311e-11 3.802842 0.625 0.073 2.692094e-07
## ENSGALG00000051696 1.229211e-11 1.856255 0.708 0.101 2.993867e-07
## SFSWAP 1.799863e-11 2.327982 0.875 0.266 4.383747e-07
## ENSGALG00000054456 2.130692e-11 2.778968 0.542 0.037 5.189512e-07
## RALYL 3.102817e-11 2.209327 1.000 0.826 7.557222e-07
## ENSGALG00000046718 3.197770e-11 2.143086 0.750 0.165 7.788489e-07
## TENM2 3.781707e-11 2.492435 1.000 0.697 9.210725e-07
## ENSGALG00000052195 5.126341e-11 1.672383 0.833 0.239 1.248572e-06
## GRIK2 6.782174e-11 1.549751 1.000 0.734 1.651866e-06
## ENSGALG00000040489 6.897756e-11 1.455289 0.625 0.083 1.680018e-06
## ENSGALG00000050475 1.017546e-10 2.510226 0.958 0.431 2.478336e-06
## IL1RAPL1 1.060596e-10 2.499740 1.000 0.624 2.583188e-06
## KCNC3 1.086072e-10 1.302920 0.833 0.202 2.645237e-06
## TTC31 1.506192e-10 3.152331 0.500 0.037 3.668481e-06
## ENSGALG00000052136 1.548700e-10 3.547875 0.792 0.229 3.772013e-06
## DIP2B 2.200792e-10 2.358536 1.000 0.725 5.360249e-06
## HECTD2 2.624013e-10 2.743858 0.708 0.147 6.391047e-06
## GCLM 2.810262e-10 1.941504 1.000 0.789 6.844675e-06
## DPF3 3.194390e-10 1.927707 1.000 0.890 7.780257e-06
## ENSGALG00000051209 3.440561e-10 3.496347 0.458 0.028 8.379831e-06
## ENSGALG00000053466 3.610762e-10 1.949586 0.875 0.349 8.794372e-06
## ENSGALG00000032941 4.381368e-10 1.899031 0.750 0.147 1.067126e-05
## DMD 4.784997e-10 2.334433 1.000 0.872 1.165434e-05
## ENSGALG00000051147 5.085186e-10 2.541557 0.583 0.073 1.238548e-05
## CACNA2D3 5.326267e-10 2.696589 0.958 0.798 1.297266e-05
## ENSGALG00000053367 6.282163e-10 1.975357 0.333 0.000 1.530084e-05
## RBM10 7.385547e-10 1.767349 1.000 0.606 1.798824e-05
## PPARGC1A 8.052807e-10 2.229544 0.958 0.560 1.961342e-05
## KIAA1217 8.830981e-10 1.921889 1.000 0.780 2.150874e-05
## EFNA5 9.812268e-10 2.970457 0.917 0.339 2.389876e-05
## JARID2 1.125351e-09 2.915558 0.917 0.532 2.740905e-05
## CRYBB1 1.141464e-09 2.518842 0.708 0.165 2.780151e-05
## ENSGALG00000051214 1.143142e-09 2.574393 0.708 0.147 2.784237e-05
## DGKB 1.363368e-09 2.756303 0.958 0.670 3.320620e-05
## ENSGALG00000047850 1.582794e-09 1.276504 0.542 0.064 3.855052e-05
## ENSGALG00000035029 1.885628e-09 2.103284 0.792 0.211 4.592637e-05
## ENSGALG00000052263 2.000561e-09 1.369934 0.458 0.037 4.872566e-05
## LRRTM4 2.008108e-09 2.425179 0.958 0.725 4.890947e-05
## LHFPL3 2.049774e-09 3.105425 0.917 0.569 4.992429e-05
## ENSGALG00000052970 2.119138e-09 2.230877 0.833 0.275 5.161372e-05
## ARPP21 2.197738e-09 1.979489 1.000 0.633 5.352811e-05
## TIA1 2.248854e-09 1.379439 0.750 0.156 5.477309e-05
## ENSGALG00000013505 2.277747e-09 1.192613 1.000 1.000 5.547681e-05
## PCDH7 2.443148e-09 1.429928 1.000 0.945 5.950532e-05
## ENSGALG00000050013 2.672354e-09 2.031550 0.917 0.367 6.508785e-05
## ENSGALG00000054038 3.368807e-09 1.192856 0.458 0.037 8.205067e-05
## PTPRD 3.492636e-09 1.918320 1.000 0.954 8.506664e-05
## CHRM3 3.874792e-09 1.555407 1.000 0.541 9.437443e-05
## PDE7A 4.018440e-09 3.067150 0.750 0.211 9.787314e-05
## ENSGALG00000019233 4.076544e-09 2.917810 0.667 0.165 9.928830e-05
## ADARB2 4.607428e-09 2.288299 0.958 0.761 1.122185e-04
## ENSGALG00000050676 4.706894e-09 2.458731 1.000 0.789 1.146411e-04
## ENSGALG00000035916 5.389835e-09 2.457959 0.958 0.844 1.312748e-04
## ROBO1 6.014130e-09 2.053683 0.958 0.651 1.464802e-04
## ENSGALG00000016495 6.073350e-09 1.444750 0.958 0.514 1.479225e-04
## CSMD1 6.292199e-09 1.029442 0.833 0.239 1.532528e-04
## ENSGALG00000049178 6.368010e-09 2.603439 0.750 0.229 1.550992e-04
## ENSGALG00000048868 6.404173e-09 3.345154 0.375 0.018 1.559800e-04
## ENSGALG00000047741 6.944580e-09 2.546809 0.750 0.239 1.691422e-04
## CACNA1C 7.388355e-09 2.709587 0.958 0.872 1.799508e-04
## ENSGALG00000052495 7.724632e-09 4.331440 0.583 0.119 1.881411e-04
## ENSGALG00000046778 7.747634e-09 2.454091 0.417 0.028 1.887014e-04
## FGF14 8.170370e-09 2.536797 0.875 0.477 1.989975e-04
## ENSGALG00000051461 8.372441e-09 2.324442 0.292 0.000 2.039192e-04
## RSRP1 8.430241e-09 1.514338 0.958 0.569 2.053269e-04
## PNISR 8.472163e-09 1.409717 1.000 0.422 2.063480e-04
## ENSGALG00000049672 8.873150e-09 2.349724 0.583 0.110 2.161144e-04
## DSCAM 9.674530e-09 2.897300 0.917 0.826 2.356329e-04
## ENSGALG00000054272 1.029266e-08 3.455631 0.500 0.064 2.506881e-04
## CDK8 1.053367e-08 2.864660 0.667 0.174 2.565581e-04
## ENSGALG00000037980 1.182646e-08 1.252871 0.500 0.055 2.880453e-04
## NHS 1.247292e-08 2.477783 0.875 0.431 3.037903e-04
## CSMD3 1.268608e-08 1.919826 1.000 0.734 3.089823e-04
## MCTP1 1.313044e-08 2.424061 0.958 0.743 3.198050e-04
## MSI2 1.613561e-08 1.646157 1.000 0.972 3.929989e-04
## AMMECR1 1.770549e-08 1.834713 0.917 0.633 4.312350e-04
## TENM4 1.774795e-08 2.595932 0.917 0.541 4.322692e-04
## ENSGALG00000054806 1.787027e-08 1.071707 1.000 0.991 4.352482e-04
## BTBD11 1.854506e-08 2.142272 0.917 0.532 4.516834e-04
## AUTS2 2.027728e-08 2.072487 1.000 0.899 4.938734e-04
## LINGO2 2.051279e-08 2.395058 0.875 0.349 4.996094e-04
## MEIS1 2.163575e-08 1.863340 1.000 0.890 5.269604e-04
## CCDC91 2.345419e-08 1.496537 1.000 0.734 5.712502e-04
## KCNQ3 2.570586e-08 2.204270 0.833 0.367 6.260919e-04
## ENSGALG00000032057 2.962961e-08 1.502306 0.750 0.275 7.216587e-04
## TAFA2 3.343830e-08 1.914828 0.833 0.404 8.144231e-04
## PPP1R10 3.389200e-08 1.914361 0.917 0.486 8.254736e-04
## ANKRD10 3.460243e-08 1.897313 0.792 0.294 8.427767e-04
## NAV3 3.510903e-08 1.890754 1.000 0.688 8.551155e-04
## MTA2 3.547611e-08 1.033870 0.625 0.147 8.640560e-04
## ENSGALG00000044866 3.564600e-08 2.185742 0.542 0.083 8.681940e-04
## EML1 3.868058e-08 2.060544 0.792 0.312 9.421041e-04
## ADGRB3 4.078859e-08 1.942784 1.000 0.743 9.934470e-04
## ENSGALG00000049915 4.339947e-08 2.251031 0.458 0.055 1.057038e-03
## ENSGALG00000052781 4.450362e-08 2.717229 0.833 0.495 1.083930e-03
## ENSGALG00000007070 4.463298e-08 2.205128 0.583 0.128 1.087081e-03
## FSTL5 4.514799e-08 2.702289 0.917 0.688 1.099624e-03
## ERC2 4.521764e-08 2.646171 1.000 0.899 1.101321e-03
## ENSGALG00000031565 4.637766e-08 1.766882 0.417 0.037 1.129574e-03
## ENSGALG00000048557 4.951592e-08 1.859740 0.917 0.725 1.206010e-03
## HSF1 5.050133e-08 3.672084 0.542 0.101 1.230010e-03
## ENSGALG00000051765 5.301257e-08 1.952935 0.917 0.596 1.291174e-03
## RYR2 5.932484e-08 1.113061 1.000 0.982 1.444916e-03
## CDH7 6.341059e-08 2.613664 0.750 0.257 1.544428e-03
## SRSF5 7.730942e-08 1.504125 0.708 0.174 1.882948e-03
## ENSGALG00000054570 7.946524e-08 3.047370 0.333 0.018 1.935455e-03
## CDH9 8.144624e-08 3.162334 0.417 0.046 1.983705e-03
## SYT1 8.260212e-08 2.230749 0.958 0.761 2.011857e-03
## GTDC1 8.375418e-08 1.790373 0.667 0.156 2.039917e-03
## CHST8 8.801381e-08 1.478250 1.000 0.587 2.143664e-03
## KHDRBS2 9.039048e-08 1.450040 0.958 0.826 2.201550e-03
## ENSGALG00000052494 9.568175e-08 2.517537 0.833 0.450 2.330425e-03
## AFF2 9.673335e-08 1.830704 0.917 0.862 2.356037e-03
## DDX17 1.018404e-07 1.787588 0.958 0.798 2.480424e-03
## ENSGALG00000028360 1.026815e-07 1.824241 0.708 0.220 2.500909e-03
## ENSGALG00000047932 1.033741e-07 3.715891 0.583 0.147 2.517779e-03
## ENSGALG00000049164 1.084592e-07 3.048759 0.250 0.000 2.641633e-03
## STX5 1.104139e-07 1.181380 0.625 0.165 2.689241e-03
## RPS6KA2 1.105654e-07 2.047989 0.917 0.394 2.692932e-03
## LRP1B 1.189275e-07 2.205493 0.917 0.578 2.896599e-03
## ENSGALG00000044020 1.212796e-07 1.506858 0.333 0.018 2.953886e-03
## ENSGALG00000046893 1.231779e-07 1.594169 0.292 0.009 3.000122e-03
## KDM4C 1.249199e-07 1.204126 0.958 0.569 3.042548e-03
## NRG2 1.315542e-07 2.763146 0.917 0.624 3.204133e-03
## ADGRB1 1.376278e-07 2.377472 0.875 0.541 3.352062e-03
## CAMK1D 1.447782e-07 2.316098 0.917 0.514 3.526217e-03
## ENSGALG00000046650 1.461358e-07 2.276375 0.417 0.046 3.559283e-03
## MND1 1.461432e-07 3.242915 0.625 0.183 3.559464e-03
## ENSGALG00000047906 1.553338e-07 1.457597 0.292 0.009 3.783309e-03
## HDAC7 1.573886e-07 1.725295 0.917 0.596 3.833356e-03
## PDE4D 1.623417e-07 2.377081 0.958 0.716 3.953993e-03
## KCNQ2 1.714944e-07 2.169122 0.958 0.881 4.176918e-03
## CCSER1 1.721002e-07 2.752131 0.875 0.532 4.191672e-03
## ENSGALG00000020322 1.772177e-07 1.268593 0.958 0.697 4.316314e-03
## ENSGALG00000053975 2.230523e-07 3.285960 0.542 0.128 5.432663e-03
## BRD1 2.268995e-07 1.619461 0.792 0.321 5.526364e-03
## ENSGALG00000029423 2.424109e-07 1.443968 0.958 0.633 5.904159e-03
## CPEB3 2.617904e-07 1.728620 0.708 0.248 6.376168e-03
## ENSGALG00000052274 2.721835e-07 1.016826 0.917 0.468 6.629302e-03
## ENSGALG00000048913 2.861939e-07 3.436680 0.375 0.037 6.970539e-03
## MAGI2 3.055710e-07 1.656451 0.958 0.844 7.442487e-03
## ZNF407 3.074371e-07 2.508879 0.833 0.376 7.487939e-03
## EML5 3.101107e-07 2.224316 0.917 0.725 7.553055e-03
## RIMS2 3.105351e-07 1.554212 1.000 0.936 7.563392e-03
## DLGAP1 3.357747e-07 2.567186 0.958 0.954 8.178128e-03
## ENSGALG00000030099 3.547046e-07 1.197966 0.542 0.119 8.639186e-03
## ENSGALG00000049016 3.632966e-07 2.267906 0.625 0.174 8.848452e-03
## NOC4L 3.699249e-07 1.931178 0.792 0.303 9.009891e-03
## GPR157 3.877926e-07 2.830970 0.625 0.211 9.445078e-03
## ERCC6 3.955528e-07 1.700799 0.750 0.248 9.634084e-03
## LRRC4C 4.130638e-07 1.729391 0.958 0.899 1.006058e-02
## PHF20L1 4.291895e-07 1.342811 0.958 0.468 1.045334e-02
## GDF11 4.629111e-07 1.012896 0.542 0.119 1.127466e-02
## TENM1 4.641144e-07 2.042212 0.875 0.523 1.130397e-02
## TAFA5 4.642261e-07 1.383924 1.000 0.972 1.130669e-02
## SCN2A 4.665871e-07 1.208461 0.958 0.596 1.136419e-02
## ABCC4 4.831027e-07 2.321378 0.708 0.257 1.176645e-02
## PLEKHA5 4.893566e-07 2.370138 0.750 0.321 1.191877e-02
## ENSGALG00000050387 4.921589e-07 2.780754 0.625 0.202 1.198702e-02
## ENSGALG00000054653 4.923065e-07 2.688246 0.625 0.193 1.199062e-02
## DMXL1 4.956058e-07 1.691864 0.750 0.349 1.207098e-02
## SKAP2 5.105529e-07 2.268170 0.833 0.459 1.243503e-02
## PDZRN4 5.106141e-07 2.170673 0.917 0.661 1.243652e-02
## ENSGALG00000050234 5.208867e-07 3.526428 0.583 0.156 1.268672e-02
## ENSGALG00000046960 5.516521e-07 1.241673 0.375 0.037 1.343604e-02
## MSH4 5.524941e-07 3.028385 0.625 0.202 1.345655e-02
## TMEM131L 5.621012e-07 2.122679 0.792 0.394 1.369054e-02
## ATP9B 5.955611e-07 1.868063 0.917 0.661 1.450549e-02
## GNE 6.185889e-07 1.397821 0.958 0.761 1.506635e-02
## PLXNA4 6.775339e-07 1.818689 0.958 0.761 1.650202e-02
## NEDD4 7.340153e-07 2.782031 0.500 0.110 1.787768e-02
## GPHN 7.520046e-07 1.120667 1.000 0.927 1.831582e-02
## RGS17 7.585514e-07 2.427875 0.833 0.477 1.847528e-02
## ENSGALG00000055071 7.719899e-07 2.676092 0.333 0.028 1.880258e-02
## CLK2 7.910761e-07 2.414351 0.542 0.128 1.926745e-02
## SCN3A 8.601482e-07 2.642162 0.792 0.394 2.094977e-02
## ENSGALG00000047303 8.866468e-07 1.263984 0.917 0.523 2.159517e-02
## SHISAL1 9.071976e-07 2.559268 1.000 0.725 2.209571e-02
## WWC1 9.633072e-07 1.808009 0.792 0.284 2.346231e-02
## RBMXL1 9.689867e-07 1.476002 0.958 0.486 2.360064e-02
## ENSGALG00000047681 1.002166e-06 1.321826 0.292 0.018 2.440876e-02
## ENSGALG00000047545 1.020564e-06 2.589459 0.500 0.101 2.485685e-02
## ENSGALG00000038432 1.196777e-06 3.938444 0.417 0.064 2.914869e-02
## ENSGALG00000052669 1.370842e-06 1.982515 0.208 0.000 3.338823e-02
## KIAA2026 1.389601e-06 1.402714 0.792 0.349 3.384513e-02
## DIP2C 1.390454e-06 1.638415 0.875 0.596 3.386589e-02
## SLC2A8 1.447920e-06 1.290537 0.792 0.284 3.526554e-02
## FBXL17 1.451335e-06 2.190716 0.708 0.321 3.534871e-02
## STS 1.467693e-06 1.632316 0.958 0.624 3.574714e-02
## ENSGALG00000054601 1.504374e-06 1.724431 0.625 0.211 3.664053e-02
## ENSGALG00000049524 1.504867e-06 1.475016 0.292 0.018 3.665254e-02
## EPB41L4A 1.582796e-06 2.196465 0.917 0.624 3.855058e-02
## SASS6 1.621421e-06 2.144516 0.417 0.064 3.949134e-02
## CACNA1H 1.652450e-06 2.303408 0.958 0.991 4.024708e-02
## SCN9A 1.679976e-06 1.288335 0.833 0.450 4.091750e-02
## RPGR 1.766135e-06 1.640488 0.875 0.596 4.301599e-02
## ENSGALG00000052834 1.796264e-06 1.350018 0.458 0.092 4.374982e-02
## ENSGALG00000033303 1.838490e-06 2.803724 0.250 0.009 4.477827e-02
## ENSGALG00000054724 1.981594e-06 1.240216 0.250 0.009 4.826371e-02
## ENSGALG00000009298 2.023682e-06 1.656429 0.708 0.303 4.928880e-02
## SNTG1 2.053465e-06 2.029216 1.000 0.954 5.001420e-02
## ENSGALG00000032452 2.075828e-06 2.237212 0.792 0.367 5.055886e-02
## DIP2A 2.110057e-06 1.358473 0.875 0.596 5.139255e-02
## ERICH1 2.387186e-06 1.900774 0.583 0.165 5.814230e-02
## DPH6 2.427466e-06 1.351161 0.958 0.651 5.912335e-02
## ENSGALG00000039727 2.494069e-06 2.313830 0.667 0.257 6.074555e-02
## KMT2D 2.502706e-06 1.471289 0.625 0.211 6.095592e-02
## SHISA9 2.504207e-06 1.081793 0.917 0.578 6.099245e-02
## RBFOX1 2.597422e-06 2.485620 0.792 0.505 6.326281e-02
## ENSGALG00000049285 2.616986e-06 1.102004 0.958 0.569 6.373930e-02
## ZNF521 2.682956e-06 1.443562 0.917 0.633 6.534608e-02
## EXOC6 2.720839e-06 1.234351 0.792 0.404 6.626875e-02
## FNBP1L 2.757231e-06 1.322284 0.875 0.532 6.715513e-02
## SETBP1 2.890167e-06 2.270851 0.958 0.927 7.039291e-02
## CNKSR2 2.925319e-06 1.452625 0.917 0.706 7.124907e-02
## CNTNAP2 2.970411e-06 1.702176 1.000 0.826 7.234733e-02
## GRIA2 3.010406e-06 1.091877 1.000 0.853 7.332145e-02
## BRSK1 3.158836e-06 1.410128 1.000 0.982 7.693662e-02
## FAR2 3.274315e-06 1.376031 0.833 0.569 7.974922e-02
## ARHGAP21 3.430890e-06 1.472767 0.917 0.569 8.356275e-02
## MBD5 3.565669e-06 2.435343 0.625 0.239 8.684542e-02
## ENSGALG00000034392 3.679252e-06 1.701675 0.833 0.569 8.961186e-02
## CNTN5 3.700128e-06 1.724348 0.875 0.532 9.012032e-02
## FHOD3 3.710175e-06 1.404104 1.000 0.780 9.036503e-02
## ZBTB49 3.827360e-06 1.880200 0.500 0.110 9.321918e-02
## CPEB2 3.872691e-06 2.091669 0.583 0.174 9.432326e-02
## TARP 4.192111e-06 2.387637 0.500 0.128 1.021031e-01
## FRMD3 4.583428e-06 1.133532 0.958 0.606 1.116340e-01
## BICD1 4.731355e-06 1.262349 0.958 0.661 1.152369e-01
## TANC2 4.833093e-06 1.858376 0.917 0.661 1.177148e-01
## ENSGALG00000037131 4.921836e-06 2.033252 0.625 0.220 1.198762e-01
## HPS3 5.029245e-06 2.217757 0.792 0.422 1.224923e-01
## ANXA2 5.071756e-06 2.435395 0.667 0.257 1.235277e-01
## JAKMIP1 5.092282e-06 1.445152 0.875 0.587 1.240276e-01
## SFMBT2 5.156035e-06 2.523303 0.667 0.284 1.255804e-01
## CCNL1 5.420576e-06 2.231038 0.667 0.257 1.320236e-01
## SEMA4D 5.453370e-06 1.543640 0.792 0.367 1.328223e-01
## ENSGALG00000043816 5.512782e-06 2.776596 0.875 0.615 1.342693e-01
## ENSGALG00000045015 6.142507e-06 2.628217 0.417 0.073 1.496069e-01
## B3GALNT2 6.426153e-06 1.155870 0.750 0.358 1.565154e-01
## RBMS2 6.434531e-06 1.279526 0.417 0.073 1.567194e-01
## WDR60 6.612564e-06 2.297078 0.583 0.202 1.610556e-01
## ENSGALG00000043243 6.704430e-06 1.221480 0.708 0.229 1.632931e-01
## GABBR2 6.733321e-06 1.843649 1.000 0.835 1.639968e-01
## MAP4K3 6.844397e-06 1.572283 1.000 0.881 1.667021e-01
## PXDN 6.932004e-06 2.459685 0.625 0.248 1.688359e-01
## PPHLN1 7.019865e-06 1.384286 1.000 0.688 1.709758e-01
## GNAL 7.054886e-06 2.258339 0.833 0.596 1.718288e-01
## KLF13 7.096312e-06 2.548433 0.333 0.046 1.728378e-01
## ATRNL1 7.432540e-06 1.764928 1.000 0.881 1.810269e-01
## MBOAT1 7.625379e-06 2.822485 0.500 0.138 1.857237e-01
## ENSGALG00000047639 7.729264e-06 2.257282 0.292 0.028 1.882539e-01
## ENSGALG00000040336 7.773642e-06 1.607431 0.458 0.101 1.893348e-01
## CCDC171 7.783683e-06 1.548978 0.875 0.587 1.895794e-01
## ENSGALG00000050499 7.892858e-06 1.483342 0.333 0.046 1.922384e-01
## ADGRL3 7.965779e-06 2.295293 0.792 0.578 1.940145e-01
## CHD9 7.983316e-06 1.355503 0.875 0.468 1.944416e-01
## TAF6L 8.185183e-06 1.231101 0.625 0.248 1.993583e-01
## DPP6 8.641263e-06 2.253857 0.917 0.780 2.104666e-01
## NLGN4Y 9.224481e-06 1.375295 1.000 0.872 2.246715e-01
## CACNB2 9.309456e-06 1.681748 1.000 0.826 2.267411e-01
## XKR4 9.424917e-06 2.417896 0.833 0.569 2.295533e-01
## PPFIA2 9.809194e-06 1.724928 0.917 0.679 2.389127e-01
## STRN3 1.018934e-05 1.729406 0.708 0.312 2.481716e-01
## EIF5A2 1.025424e-05 -1.218211 0.958 0.954 2.497522e-01
## ENSGALG00000038579 1.043628e-05 1.459243 0.500 0.119 2.541861e-01
## RBMS3 1.068357e-05 2.332852 0.958 0.752 2.602091e-01
## MAMLD1 1.101969e-05 2.107703 0.833 0.596 2.683956e-01
## MLLT10 1.120760e-05 1.955422 0.583 0.193 2.729724e-01
## LDLRAD4 1.123986e-05 1.682771 0.833 0.550 2.737581e-01
## SUGCT 1.132106e-05 2.133241 0.917 0.697 2.757358e-01
## FBXL4 1.137082e-05 1.825356 0.625 0.248 2.769478e-01
## PTPRO 1.146436e-05 1.489906 0.875 0.642 2.792261e-01
## NBEA 1.187696e-05 1.373228 1.000 0.927 2.892751e-01
## UNC79 1.261671e-05 1.619387 0.917 0.697 3.072925e-01
## NOL4 1.279843e-05 2.016049 0.750 0.440 3.117185e-01
## EML4 1.279991e-05 2.500487 0.792 0.505 3.117547e-01
## ENSGALG00000051615 1.297774e-05 1.672627 0.417 0.083 3.160859e-01
## RORA 1.308498e-05 1.046895 1.000 0.807 3.186977e-01
## RGS7 1.309621e-05 1.074114 1.000 0.872 3.189712e-01
## ENSGALG00000003605 1.328704e-05 1.461071 0.833 0.514 3.236191e-01
## GABRA3 1.379366e-05 2.041558 0.667 0.275 3.359585e-01
## FAM135B 1.380158e-05 1.382418 0.833 0.505 3.361512e-01
## PARD3 1.435474e-05 1.898280 0.875 0.550 3.496240e-01
## ALKAL2 1.441407e-05 2.477747 0.250 0.018 3.510691e-01
## ENSGALG00000052619 1.453014e-05 1.522209 0.458 0.101 3.538960e-01
## SETD3 1.506026e-05 1.266950 0.667 0.248 3.668076e-01
## ENSGALG00000053819 1.526299e-05 1.660775 0.542 0.147 3.717454e-01
## PPP1R14C 1.613119e-05 2.217538 0.625 0.284 3.928912e-01
## GRM5 1.675698e-05 1.943080 0.833 0.505 4.081330e-01
## ADCYAP1R1 1.699161e-05 1.983691 0.958 0.706 4.138477e-01
## ENSGALG00000047437 1.700307e-05 2.153883 0.167 0.000 4.141267e-01
## ENSGALG00000047691 1.700307e-05 2.123153 0.167 0.000 4.141267e-01
## SORBS2 1.704415e-05 2.122635 0.833 0.514 4.151274e-01
## SLC8A3 1.766856e-05 1.191844 0.750 0.312 4.303355e-01
## DTNA 1.767493e-05 1.585095 0.792 0.413 4.304906e-01
## BRINP3 1.768180e-05 1.887805 0.917 0.725 4.306580e-01
## ROBO2 1.805654e-05 1.497435 0.917 0.606 4.397851e-01
## PPP4R4 1.836331e-05 2.205391 0.792 0.440 4.472567e-01
## ENSGALG00000031548 1.875799e-05 1.740730 0.708 0.321 4.568695e-01
## RSU1 1.940451e-05 1.438964 0.833 0.495 4.726163e-01
## ZDHHC23 1.948525e-05 2.612326 0.208 0.009 4.745828e-01
## FAT4 2.011668e-05 1.492292 0.708 0.385 4.899619e-01
## TRAPPC8 2.086682e-05 1.046566 0.708 0.257 5.082324e-01
## ENSGALG00000037747 2.212390e-05 1.809586 0.500 0.138 5.388498e-01
## ENSGALG00000034397 2.230312e-05 1.701922 0.375 0.064 5.432148e-01
## GPCPD1 2.272422e-05 1.189427 0.958 0.706 5.534712e-01
## ATP8B2 2.380654e-05 2.242550 0.375 0.073 5.798321e-01
## ARHGAP39 2.443731e-05 1.318110 0.958 0.853 5.951950e-01
## ENSGALG00000012941 2.452186e-05 1.876007 0.833 0.569 5.972544e-01
## TSPAN4 2.555994e-05 1.493875 1.000 0.954 6.225379e-01
## ABLIM1 2.589997e-05 1.285214 0.917 0.716 6.308196e-01
## JMJD1C 2.623708e-05 1.158603 0.917 0.771 6.390304e-01
## ATP8A2 2.650892e-05 1.350699 0.958 0.789 6.456512e-01
## HCN1 2.654967e-05 2.326608 0.708 0.376 6.466438e-01
## ENSGALG00000016908 2.691689e-05 1.102244 1.000 0.954 6.555877e-01
## ZNF804A 2.811300e-05 1.158717 1.000 0.881 6.847203e-01
## CPLANE1 2.851167e-05 1.108247 0.708 0.275 6.944303e-01
## ENSGALG00000049883 2.956652e-05 1.131500 0.292 0.037 7.201221e-01
## CLASP2 3.114860e-05 1.290466 0.917 0.670 7.586552e-01
## ENSGALG00000052555 3.119932e-05 3.677672 0.292 0.037 7.598906e-01
## ADAMTS2 3.594946e-05 1.790843 0.958 0.853 8.755850e-01
## MKLN1 3.673789e-05 1.003050 0.833 0.477 8.947881e-01
## ENSGALG00000029702 3.775648e-05 2.518404 0.583 0.220 9.195968e-01
## REV3L 3.792014e-05 1.503875 0.875 0.541 9.235829e-01
## TMC1 3.855369e-05 2.372394 0.417 0.101 9.390136e-01
## HECW2 3.897650e-05 2.216002 0.750 0.486 9.493116e-01
## LAMA3 4.054887e-05 2.390898 0.708 0.376 9.876084e-01
## NUP93 4.059361e-05 1.800474 0.917 0.624 9.886979e-01
## ATXN7L1 4.193119e-05 1.652518 0.708 0.339 1.000000e+00
## UHRF2 4.437943e-05 1.776166 0.708 0.339 1.000000e+00
## RNPC3 4.515199e-05 1.743651 0.625 0.266 1.000000e+00
## TLK1 4.606174e-05 1.539272 0.750 0.468 1.000000e+00
## PPP3CA 4.618006e-05 1.111323 0.917 0.706 1.000000e+00
## SPNS2 4.712948e-05 2.962453 0.583 0.220 1.000000e+00
## ANKRD26 4.726417e-05 1.601604 0.667 0.321 1.000000e+00
## DYRK1A 4.813459e-05 1.096323 0.667 0.266 1.000000e+00
## PTPRN2 4.879014e-05 1.459679 0.958 0.917 1.000000e+00
## SBF2 4.892169e-05 2.127351 0.958 0.789 1.000000e+00
## WWC3 4.912770e-05 1.632590 0.667 0.330 1.000000e+00
## ENSGALG00000054059 4.935040e-05 2.178465 0.333 0.055 1.000000e+00
## TNKS 4.951196e-05 1.146757 0.833 0.468 1.000000e+00
## DACH1 4.984688e-05 2.310404 0.875 0.670 1.000000e+00
## NCAM2 5.174142e-05 1.130622 0.917 0.716 1.000000e+00
## CAST 5.317555e-05 1.261054 0.875 0.541 1.000000e+00
## ADAMTS19 5.399699e-05 2.373133 0.875 0.679 1.000000e+00
## SLC1A1 5.459074e-05 1.015898 0.708 0.367 1.000000e+00
## DNAJC1 5.461739e-05 1.029453 0.708 0.330 1.000000e+00
## KIF13A 5.559273e-05 2.323218 0.583 0.248 1.000000e+00
## BRCA2 5.650541e-05 1.970109 0.458 0.138 1.000000e+00
## ENSGALG00000053962 5.709637e-05 2.432100 0.250 0.028 1.000000e+00
## ENSGALG00000052350 5.709637e-05 1.473342 0.250 0.028 1.000000e+00
## URI1 5.722417e-05 1.064277 0.792 0.477 1.000000e+00
## MYT1L 5.766149e-05 1.329812 0.917 0.743 1.000000e+00
## FRMD4A 5.881854e-05 2.274866 0.500 0.165 1.000000e+00
## SLC4A10 5.970625e-05 1.621602 0.750 0.532 1.000000e+00
## MSTO1 6.014862e-05 1.264046 0.667 0.294 1.000000e+00
## PRTFDC1 6.416322e-05 1.745673 0.542 0.183 1.000000e+00
## AGRN 6.488236e-05 1.603286 0.917 0.761 1.000000e+00
## KIAA1841 6.672785e-05 1.212918 0.917 0.642 1.000000e+00
## ENSGALG00000052367 6.771116e-05 1.693940 0.250 0.028 1.000000e+00
## GALNTL6 6.780969e-05 3.109743 0.792 0.532 1.000000e+00
## RCC2 6.872666e-05 1.533010 0.500 0.156 1.000000e+00
## ATAD5 6.884847e-05 2.330711 0.375 0.083 1.000000e+00
## ENSGALG00000054366 7.101417e-05 1.085182 0.542 0.193 1.000000e+00
## ENSGALG00000053196 7.175488e-05 1.937931 0.375 0.083 1.000000e+00
## ARHGEF26 7.288090e-05 2.404974 0.583 0.220 1.000000e+00
## GALNT3 7.435589e-05 1.611519 0.583 0.193 1.000000e+00
## GRID2 7.447170e-05 1.867156 0.875 0.670 1.000000e+00
## ADAM22 7.500424e-05 1.386992 0.875 0.743 1.000000e+00
## GRM8 7.694794e-05 1.368957 0.917 0.651 1.000000e+00
## NRCAM 7.728887e-05 1.539518 0.875 0.596 1.000000e+00
## LUC7L3 7.764154e-05 1.033794 0.917 0.743 1.000000e+00
## MIPOL1 7.876794e-05 1.305325 0.833 0.569 1.000000e+00
## DDX55 7.917538e-05 2.320864 0.417 0.110 1.000000e+00
## TBC1D4 8.105216e-05 1.318999 0.875 0.514 1.000000e+00
## EBF1 8.188982e-05 1.990714 0.917 0.862 1.000000e+00
## ENSGALG00000052996 8.218544e-05 1.796380 0.292 0.046 1.000000e+00
## ELAVL2 8.617090e-05 1.117750 0.792 0.413 1.000000e+00
## BRCA1 8.715378e-05 1.263958 0.833 0.532 1.000000e+00
## EFNB2 8.764577e-05 2.010454 0.542 0.211 1.000000e+00
## COCH 8.767282e-05 1.343392 0.417 0.101 1.000000e+00
## OBI1 8.781698e-05 1.344978 0.583 0.211 1.000000e+00
## ENSGALG00000053372 8.857947e-05 3.163213 0.458 0.156 1.000000e+00
## PTCH1 8.861904e-05 1.863199 0.583 0.239 1.000000e+00
## ADARB1 8.871256e-05 2.155506 0.875 0.633 1.000000e+00
## LRBA 9.297174e-05 1.156870 0.958 0.872 1.000000e+00
## IGSF9B 9.493792e-05 1.361039 0.625 0.312 1.000000e+00
## UBE2E2 9.652655e-05 1.119596 0.917 0.596 1.000000e+00
## MORC2 1.002264e-04 1.490131 0.750 0.413 1.000000e+00
## RARG 1.028583e-04 1.106081 0.583 0.239 1.000000e+00
## ENSGALG00000015576 1.121673e-04 1.933847 0.542 0.211 1.000000e+00
## AKAP6 1.137450e-04 1.169356 1.000 0.917 1.000000e+00
## MON2 1.186516e-04 1.181572 0.708 0.422 1.000000e+00
## SLC6A15 1.198610e-04 1.593677 0.750 0.477 1.000000e+00
## TAOK1 1.252593e-04 1.625524 0.917 0.761 1.000000e+00
## AMY2A 1.305588e-04 3.298083 0.208 0.018 1.000000e+00
## CLK4 1.332659e-04 1.445130 0.667 0.330 1.000000e+00
## SIPA1L1 1.349310e-04 1.051325 1.000 0.633 1.000000e+00
## HOOK1 1.356741e-04 3.046894 0.333 0.073 1.000000e+00
## ENSGALG00000051200 1.388084e-04 2.076854 0.208 0.018 1.000000e+00
## ENSGALG00000050608 1.398476e-04 1.250799 0.292 0.046 1.000000e+00
## AFMID 1.417094e-04 1.888048 0.458 0.147 1.000000e+00
## ENSGALG00000049600 1.418114e-04 1.961925 0.458 0.156 1.000000e+00
## EP400 1.426288e-04 1.825271 0.792 0.578 1.000000e+00
## DONSON 1.475472e-04 2.081729 0.208 0.018 1.000000e+00
## SLC30A7 1.514533e-04 1.194970 0.583 0.211 1.000000e+00
## ACTN2 1.515518e-04 2.093651 0.458 0.147 1.000000e+00
## ENSGALG00000007395 1.537751e-04 -1.797734 0.292 0.046 1.000000e+00
## ENSGALG00000011444 1.612189e-04 1.445281 0.292 0.046 1.000000e+00
## KLHL1 1.642988e-04 1.273309 0.750 0.431 1.000000e+00
## NKTR 1.643241e-04 1.376075 0.958 0.936 1.000000e+00
## ENSGALG00000050645 1.666014e-04 2.396676 0.208 0.018 1.000000e+00
## ENSGALG00000046731 1.666014e-04 1.383484 0.208 0.018 1.000000e+00
## NTRK2 1.774115e-04 1.846342 0.958 0.844 1.000000e+00
## ESRRB 1.776093e-04 1.599757 0.708 0.477 1.000000e+00
## TRIO 1.823080e-04 1.025986 0.958 0.780 1.000000e+00
## SUSD4 1.851644e-04 1.635265 0.917 0.881 1.000000e+00
## ENSGALG00000053108 1.871994e-04 2.470964 0.292 0.055 1.000000e+00
## ENSGALG00000048836 1.881820e-04 2.705251 0.708 0.459 1.000000e+00
## ARL15 1.898405e-04 1.802223 0.917 0.780 1.000000e+00
## CCDC85A 1.916767e-04 1.827431 0.708 0.376 1.000000e+00
## EXT1 1.961564e-04 2.921031 0.583 0.321 1.000000e+00
## BBS7 1.970967e-04 1.344683 0.458 0.138 1.000000e+00
## BAZ2B 1.985974e-04 1.120837 0.917 0.725 1.000000e+00
## ENSGALG00000048583 2.090423e-04 2.135698 0.125 0.000 1.000000e+00
## ENSGALG00000049077 2.090423e-04 2.091749 0.125 0.000 1.000000e+00
## ENSGALG00000052823 2.090423e-04 1.744667 0.125 0.000 1.000000e+00
## ENSGALG00000048595 2.090423e-04 1.738731 0.125 0.000 1.000000e+00
## ENSGALG00000048779 2.090423e-04 1.302054 0.125 0.000 1.000000e+00
## ENSGALG00000002561 2.090423e-04 1.159468 0.125 0.000 1.000000e+00
## ENSGALG00000053276 2.090423e-04 1.027693 0.125 0.000 1.000000e+00
## ENSGALG00000052696 2.097255e-04 2.196027 0.625 0.312 1.000000e+00
## NARS2 2.101627e-04 2.121669 0.458 0.156 1.000000e+00
## OVCH2 2.116164e-04 2.940010 0.250 0.037 1.000000e+00
## ENSGALG00000015032 2.140409e-04 1.526100 0.375 0.092 1.000000e+00
## REEP2 2.170763e-04 1.922651 0.458 0.156 1.000000e+00
## ST7 2.181655e-04 1.259050 0.750 0.532 1.000000e+00
## TA3 2.250928e-04 1.543329 0.875 0.624 1.000000e+00
## GABRG3 2.288605e-04 1.502121 0.958 0.890 1.000000e+00
## ENSGALG00000055112 2.288605e-04 1.348614 0.958 0.890 1.000000e+00
## CRYGN 2.295673e-04 1.176551 0.167 0.009 1.000000e+00
## CSMD2 2.323551e-04 1.936591 0.958 0.917 1.000000e+00
## SMPD3 2.340259e-04 2.574383 0.250 0.037 1.000000e+00
## TFPI 2.391232e-04 1.518522 0.708 0.349 1.000000e+00
## PHIP 2.410328e-04 1.080136 0.750 0.495 1.000000e+00
## TTC14 2.416504e-04 2.070776 0.333 0.073 1.000000e+00
## DIPK1A 2.448721e-04 2.432161 0.292 0.055 1.000000e+00
## ST6GALNAC1 2.461003e-04 2.998124 0.167 0.009 1.000000e+00
## ENSGALG00000023179 2.484877e-04 1.743813 0.667 0.358 1.000000e+00
## EHMT1 2.511635e-04 1.098631 0.875 0.615 1.000000e+00
## PCF11 2.605483e-04 1.651013 0.583 0.248 1.000000e+00
## GLE1 2.664783e-04 2.212812 0.375 0.101 1.000000e+00
## ENSGALG00000051497 2.677533e-04 1.954315 0.500 0.202 1.000000e+00
## ENSGALG00000052155 2.727605e-04 2.705774 0.333 0.073 1.000000e+00
## CP 2.789478e-04 1.328391 0.583 0.284 1.000000e+00
## ENSGALG00000049383 2.920500e-04 1.259242 0.792 0.495 1.000000e+00
## MFN1 2.947536e-04 1.647045 0.708 0.367 1.000000e+00
## FNIP2 2.969144e-04 1.515199 0.583 0.275 1.000000e+00
## DCUN1D3 2.980073e-04 2.757451 0.417 0.128 1.000000e+00
## LUC7L 2.984702e-04 1.167635 0.750 0.358 1.000000e+00
## AGMO 2.991530e-04 1.332668 0.792 0.550 1.000000e+00
## MAN2A1 3.034695e-04 2.495121 0.542 0.257 1.000000e+00
## BTAF1 3.069493e-04 1.412273 0.500 0.183 1.000000e+00
## MACF1 3.082164e-04 1.017541 0.958 0.817 1.000000e+00
## ZMIZ1 3.140763e-04 1.364052 0.875 0.771 1.000000e+00
## ENSGALG00000043903 3.201031e-04 1.633434 0.333 0.073 1.000000e+00
## PFKFB3 3.251579e-04 1.627946 0.625 0.321 1.000000e+00
## ABHD2 3.291298e-04 2.546215 0.500 0.202 1.000000e+00
## EPHA7 3.306624e-04 2.011072 0.583 0.275 1.000000e+00
## NR2C1 3.420305e-04 1.793987 0.667 0.367 1.000000e+00
## IFT172 3.423653e-04 2.283273 0.625 0.321 1.000000e+00
## LCMT2 3.439209e-04 2.156649 0.458 0.165 1.000000e+00
## GPR135 3.448584e-04 1.130563 0.458 0.138 1.000000e+00
## CRPPA 3.465320e-04 1.367929 0.500 0.165 1.000000e+00
## POLG 3.465612e-04 1.642710 0.333 0.073 1.000000e+00
## PRKAR2B 3.495375e-04 1.944415 0.833 0.697 1.000000e+00
## ENSGALG00000001289 3.542521e-04 1.062369 0.667 0.284 1.000000e+00
## RUNX1T1 3.555223e-04 1.492684 0.750 0.523 1.000000e+00
## PDE10A 3.607995e-04 2.215040 0.750 0.541 1.000000e+00
## NPAS3 3.623132e-04 1.199884 1.000 0.972 1.000000e+00
## ACER3 3.762365e-04 1.438060 0.625 0.303 1.000000e+00
## GRIA3 4.010825e-04 1.293509 0.917 0.881 1.000000e+00
## SORBS1 4.023314e-04 1.365651 0.833 0.569 1.000000e+00
## ENSGALG00000015345 4.109108e-04 1.651763 0.375 0.101 1.000000e+00
## FYN 4.262931e-04 1.079179 0.750 0.431 1.000000e+00
## INPP4B 4.274288e-04 1.635274 0.958 0.798 1.000000e+00
## ENSGALG00000054359 4.308090e-04 3.236183 0.292 0.064 1.000000e+00
## RTTN 4.308090e-04 2.389897 0.292 0.064 1.000000e+00
## ZMYND12 4.308090e-04 1.846112 0.292 0.064 1.000000e+00
## TMEM108 4.371969e-04 2.412484 0.667 0.431 1.000000e+00
## SRD5A2 4.444461e-04 1.276276 0.375 0.110 1.000000e+00
## SLC37A4 4.465718e-04 1.521780 0.542 0.220 1.000000e+00
## SLC35F1 4.482300e-04 1.543019 0.625 0.367 1.000000e+00
## CTDP1 4.562164e-04 2.033217 0.708 0.450 1.000000e+00
## GAS2 4.673010e-04 1.293194 0.917 0.771 1.000000e+00
## SNCAIP 4.741312e-04 1.033387 0.708 0.440 1.000000e+00
## TSSC1 4.745906e-04 1.246219 0.792 0.514 1.000000e+00
## SECISBP2 4.753174e-04 1.147051 0.583 0.248 1.000000e+00
## KMT2E 4.933030e-04 1.578059 0.833 0.578 1.000000e+00
## ENSGALG00000049151 4.972103e-04 2.128861 0.667 0.394 1.000000e+00
## ENSGALG00000026460 5.070817e-04 1.862618 0.542 0.239 1.000000e+00
## ENSGALG00000052222 5.118734e-04 1.433364 0.458 0.165 1.000000e+00
## LRRC7 5.156722e-04 3.358940 0.625 0.431 1.000000e+00
## SF3A3 5.192261e-04 1.224474 0.708 0.349 1.000000e+00
## ENSGALG00000047784 5.291825e-04 1.944076 0.292 0.064 1.000000e+00
## OLFM3 5.419027e-04 1.017514 0.917 0.642 1.000000e+00
## ENSGALG00000048389 5.458448e-04 2.665438 0.208 0.028 1.000000e+00
## ZNF236 5.464873e-04 1.829792 0.375 0.110 1.000000e+00
## L3HYPDH 5.493614e-04 1.110872 0.458 0.147 1.000000e+00
## NBEAL1 5.578414e-04 1.830596 0.542 0.211 1.000000e+00
## ENSGALG00000042992 5.732049e-04 1.406144 0.792 0.615 1.000000e+00
## ENSGALG00000046419 5.753213e-04 2.819430 0.208 0.028 1.000000e+00
## UBA2 5.772832e-04 1.313625 0.458 0.165 1.000000e+00
## SPATA6 6.091740e-04 1.065839 0.792 0.514 1.000000e+00
## NAV1 6.195898e-04 1.899788 0.625 0.312 1.000000e+00
## ENSGALG00000051162 6.403243e-04 2.019189 0.500 0.220 1.000000e+00
## CHST11 6.425853e-04 1.906841 0.708 0.495 1.000000e+00
## TBC1D5 6.434597e-04 1.726067 0.750 0.606 1.000000e+00
## OSBPL5 6.441825e-04 1.339031 0.917 0.798 1.000000e+00
## ILK 6.511455e-04 1.015872 0.750 0.394 1.000000e+00
## IGSF11 6.518361e-04 1.546002 0.750 0.541 1.000000e+00
## TBC1D30 6.580949e-04 1.772934 0.750 0.569 1.000000e+00
## KDM3B 6.639772e-04 1.052648 0.792 0.550 1.000000e+00
## ASAP1 6.665396e-04 1.521327 0.792 0.642 1.000000e+00
## FYB2 6.763464e-04 3.403888 0.250 0.046 1.000000e+00
## PHC1 6.763464e-04 3.105285 0.250 0.046 1.000000e+00
## ENSGALG00000047072 6.763464e-04 2.745003 0.250 0.046 1.000000e+00
## SFXN2 6.763464e-04 1.972172 0.250 0.046 1.000000e+00
## NNT 6.770431e-04 1.094034 0.708 0.358 1.000000e+00
## CD109 6.878254e-04 2.081539 0.708 0.440 1.000000e+00
## ANKRD28 6.918950e-04 1.794347 0.500 0.202 1.000000e+00
## CAMK4 7.257508e-04 1.331956 0.958 0.817 1.000000e+00
## ENSGALG00000010030 7.310581e-04 1.148829 0.292 0.064 1.000000e+00
## KAZN 7.320588e-04 1.075487 0.708 0.468 1.000000e+00
## MAP7 7.396560e-04 1.071257 0.875 0.697 1.000000e+00
## ENSGALG00000054322 7.410976e-04 2.071836 0.375 0.110 1.000000e+00
## ENSGALG00000035935 7.511476e-04 1.686585 0.750 0.569 1.000000e+00
## NIP7 7.524554e-04 -1.569121 0.125 0.514 1.000000e+00
## KCNK10 7.567352e-04 2.206156 0.625 0.339 1.000000e+00
## SEMA6A 7.621424e-04 1.097276 0.833 0.606 1.000000e+00
## ARHGAP17 7.679859e-04 2.242019 0.708 0.477 1.000000e+00
## CKAP4 7.731111e-04 2.493895 0.250 0.046 1.000000e+00
## CCDC88A 7.801387e-04 1.386831 0.833 0.688 1.000000e+00
## FBN2 7.883443e-04 2.422138 0.625 0.404 1.000000e+00
## ENSGALG00000048343 7.923053e-04 1.367655 0.375 0.110 1.000000e+00
## SLC44A1 7.985426e-04 1.927660 0.583 0.339 1.000000e+00
## SH3PXD2A 8.068513e-04 1.696948 0.917 0.780 1.000000e+00
## CACNA1B 8.147151e-04 1.076228 0.917 0.798 1.000000e+00
## ENSGALG00000044064 8.233072e-04 1.231356 0.833 0.697 1.000000e+00
## UBAP1 8.250387e-04 1.220291 0.500 0.229 1.000000e+00
## ENSGALG00000051720 8.269758e-04 1.515120 0.208 0.028 1.000000e+00
## UTP3 8.272764e-04 1.477665 0.417 0.138 1.000000e+00
## PRRG1 8.302781e-04 1.684597 0.500 0.211 1.000000e+00
## MRPL33 8.330793e-04 -1.158772 0.542 0.817 1.000000e+00
## ENSGALG00000049861 8.445997e-04 2.291159 0.250 0.046 1.000000e+00
## PRKN 8.624832e-04 1.502725 0.833 0.743 1.000000e+00
## PMVK 8.753027e-04 1.617873 0.375 0.110 1.000000e+00
## SMCHD1 8.812618e-04 1.758823 0.917 0.752 1.000000e+00
## ENSGALG00000048867 8.913885e-04 1.524692 0.292 0.064 1.000000e+00
## PPP4R1 8.939589e-04 2.417517 0.458 0.174 1.000000e+00
## CACNA1D 9.035476e-04 1.611500 0.708 0.440 1.000000e+00
## EXOC6B 9.512006e-04 1.453033 0.792 0.624 1.000000e+00
## ENSGALG00000053312 9.642034e-04 1.099783 0.292 0.064 1.000000e+00
## THSD7B 9.655981e-04 3.014803 0.667 0.468 1.000000e+00
## CEP170 9.742180e-04 1.063761 0.917 0.697 1.000000e+00
## ENSGALG00000051929 9.909453e-04 1.616996 0.792 0.514 1.000000e+00
## ZCCHC7 1.008253e-03 1.253117 0.667 0.422 1.000000e+00
## ENSGALG00000031244 1.022631e-03 2.376874 0.500 0.248 1.000000e+00
## CERS6 1.026756e-03 2.312217 0.625 0.330 1.000000e+00
## ENSGALG00000016837 1.029242e-03 1.487004 0.750 0.624 1.000000e+00
## TRNAU1AP 1.032202e-03 1.481212 0.333 0.092 1.000000e+00
## ENSGALG00000049165 1.032927e-03 2.093898 0.667 0.367 1.000000e+00
## GMDS 1.057562e-03 1.612076 1.000 0.890 1.000000e+00
## APBA1 1.068608e-03 1.849087 0.667 0.450 1.000000e+00
## SLC12A2 1.082970e-03 1.732805 0.542 0.266 1.000000e+00
## TRABD 1.083194e-03 1.178330 0.417 0.128 1.000000e+00
## CTTNBP2 1.084043e-03 1.491377 0.750 0.560 1.000000e+00
## ENSGALG00000001948 1.103183e-03 1.027646 0.708 0.413 1.000000e+00
## CDADC1 1.126632e-03 1.126598 0.292 0.064 1.000000e+00
## SPIRE1 1.129147e-03 1.915287 0.750 0.550 1.000000e+00
## GALC 1.138558e-03 2.113835 0.542 0.266 1.000000e+00
## DOCK11 1.140970e-03 1.199616 0.875 0.743 1.000000e+00
## ENSGALG00000048236 1.164374e-03 1.003444 0.500 0.239 1.000000e+00
## PTBP2 1.169095e-03 1.121171 0.667 0.321 1.000000e+00
## FAM149B1 1.185942e-03 1.516894 0.333 0.092 1.000000e+00
## ELOVL1 1.196303e-03 1.574901 0.250 0.046 1.000000e+00
## DAPK1 1.201363e-03 1.194408 0.875 0.670 1.000000e+00
## GFOD1 1.203821e-03 1.452189 0.583 0.330 1.000000e+00
## RBMS1 1.223784e-03 1.039664 0.917 0.927 1.000000e+00
## NUP155 1.227197e-03 1.113692 0.500 0.202 1.000000e+00
## EXOC7 1.232507e-03 1.243427 0.625 0.321 1.000000e+00
## VEGFC 1.239984e-03 1.866675 0.583 0.312 1.000000e+00
## TFRC 1.266802e-03 1.321588 0.458 0.165 1.000000e+00
## MAP3K3 1.271787e-03 1.759129 0.750 0.495 1.000000e+00
## PAN3 1.273630e-03 2.249887 0.583 0.349 1.000000e+00
## ENSGALG00000011177 1.285658e-03 1.303613 0.500 0.183 1.000000e+00
## ENSGALG00000048048 1.312648e-03 2.861911 0.167 0.018 1.000000e+00
## ADMP 1.312648e-03 2.098050 0.167 0.018 1.000000e+00
## CNEP1R1 1.348612e-03 2.219071 0.375 0.119 1.000000e+00
## ATP13A2 1.364730e-03 1.441982 0.458 0.174 1.000000e+00
## STRADA 1.380427e-03 1.666789 0.625 0.294 1.000000e+00
## NAV2 1.388939e-03 2.035821 0.792 0.697 1.000000e+00
## ENSGALG00000048943 1.388983e-03 2.430119 0.167 0.018 1.000000e+00
## LAMP3 1.392162e-03 1.954780 0.375 0.128 1.000000e+00
## ZFAND4 1.403096e-03 1.079072 0.458 0.174 1.000000e+00
## ENSGALG00000026811 1.421095e-03 2.270092 0.458 0.183 1.000000e+00
## ANKRD13A 1.448297e-03 1.628028 0.417 0.156 1.000000e+00
## METTL2B 1.456567e-03 1.834807 0.333 0.092 1.000000e+00
## NRP2 1.464696e-03 1.388086 0.583 0.303 1.000000e+00
## FAM163B 1.470689e-03 1.002354 0.417 0.138 1.000000e+00
## C20orf194 1.474102e-03 1.428252 0.667 0.413 1.000000e+00
## ESYT1 1.490524e-03 1.117726 0.417 0.156 1.000000e+00
## PDS5A 1.538124e-03 1.131568 0.875 0.688 1.000000e+00
## SRFBP1 1.539450e-03 1.264360 0.500 0.220 1.000000e+00
## RAPGEF2 1.548325e-03 1.106851 0.917 0.761 1.000000e+00
## NR3C2 1.552646e-03 1.287715 0.833 0.550 1.000000e+00
## ENSGALG00000050107 1.554073e-03 1.838897 0.167 0.018 1.000000e+00
## ENSGALG00000017072 1.554073e-03 1.246239 0.167 0.018 1.000000e+00
## LMLN 1.554073e-03 1.165060 0.167 0.018 1.000000e+00
## ENSGALG00000005475 1.584504e-03 1.013877 0.458 0.183 1.000000e+00
## CPNE4 1.627623e-03 1.254468 0.958 0.945 1.000000e+00
## CARD9 1.637340e-03 2.202610 0.250 0.055 1.000000e+00
## ENSGALG00000048667 1.651726e-03 1.807441 0.750 0.560 1.000000e+00
## KHDRBS3 1.683021e-03 2.221031 0.458 0.193 1.000000e+00
## CRIM1 1.696499e-03 1.005001 0.792 0.615 1.000000e+00
## POF1B 1.704539e-03 2.534275 0.250 0.055 1.000000e+00
## ENSGALG00000047258 1.742634e-03 1.415763 0.208 0.037 1.000000e+00
## CNTNAP5 1.750518e-03 1.739316 0.792 0.761 1.000000e+00
## KIF13B 1.752894e-03 1.684539 0.625 0.358 1.000000e+00
## ENSGALG00000014734 1.817844e-03 1.421989 0.542 0.330 1.000000e+00
## ENSGALG00000051730 1.835811e-03 1.631679 0.167 0.018 1.000000e+00
## ICE2 1.836591e-03 1.212152 0.625 0.339 1.000000e+00
## ERCC8 1.845300e-03 1.329930 0.458 0.174 1.000000e+00
## KCNT2 1.940596e-03 1.262818 0.542 0.303 1.000000e+00
## VLDLR 1.952739e-03 1.197168 0.417 0.147 1.000000e+00
## AFF3 1.972625e-03 1.402823 0.875 0.817 1.000000e+00
## ENSGALG00000016971 1.983891e-03 1.147832 0.583 0.284 1.000000e+00
## ENSGALG00000001244 1.997516e-03 2.526502 0.208 0.037 1.000000e+00
## ENSGALG00000054683 1.997516e-03 2.505500 0.208 0.037 1.000000e+00
## HK3 1.999486e-03 1.410620 0.250 0.055 1.000000e+00
## TRRAP 2.003189e-03 1.852607 0.708 0.550 1.000000e+00
## VPS13A 2.016299e-03 1.177805 0.917 0.688 1.000000e+00
## ADGRA3 2.028616e-03 1.776336 0.500 0.239 1.000000e+00
## ENSGALG00000035762 2.036879e-03 1.044516 0.333 0.092 1.000000e+00
## PPP1R21 2.054703e-03 1.236314 0.625 0.339 1.000000e+00
## ENSGALG00000047936 2.064104e-03 2.900747 0.375 0.128 1.000000e+00
## ESRRG 2.107567e-03 1.097493 1.000 0.972 1.000000e+00
## AP3B1 2.155426e-03 1.539736 0.792 0.560 1.000000e+00
## NFIA 2.158008e-03 1.279596 0.833 0.716 1.000000e+00
## ENSGALG00000052918 2.163900e-03 1.791865 0.250 0.055 1.000000e+00
## SRSF11 2.180793e-03 1.164104 0.750 0.606 1.000000e+00
## SLC26A5 2.185986e-03 1.242741 0.208 0.037 1.000000e+00
## ENSGALG00000051819 2.190618e-03 2.349080 0.375 0.128 1.000000e+00
## DDX60 2.196727e-03 1.700730 0.583 0.349 1.000000e+00
## OSBPL10 2.198781e-03 1.248920 0.708 0.560 1.000000e+00
## ENSGALG00000053282 2.225981e-03 1.898328 0.583 0.303 1.000000e+00
## TET2 2.250869e-03 1.311958 0.500 0.220 1.000000e+00
## ENSGALG00000052045 2.289782e-03 2.402825 0.375 0.138 1.000000e+00
## EIF2AK1 2.310317e-03 1.312841 0.542 0.248 1.000000e+00
## ENSGALG00000031794 2.314293e-03 1.246662 0.417 0.147 1.000000e+00
## TOGARAM1 2.324204e-03 2.038048 0.375 0.128 1.000000e+00
## ENSGALG00000015675 2.353813e-03 2.112483 0.417 0.165 1.000000e+00
## PPM1H 2.388129e-03 1.470061 0.667 0.440 1.000000e+00
## GLI1 2.390629e-03 1.842883 0.208 0.037 1.000000e+00
## ELMO3 2.400558e-03 1.269458 0.333 0.092 1.000000e+00
## ZNF618 2.415967e-03 1.121200 1.000 0.982 1.000000e+00
## WDFY2 2.427533e-03 1.689374 0.667 0.385 1.000000e+00
## ROCK1 2.479987e-03 2.395494 0.625 0.394 1.000000e+00
## ITGA9 2.481699e-03 2.231443 0.792 0.541 1.000000e+00
## RARB 2.482743e-03 1.535119 0.875 0.688 1.000000e+00
## PAPD7 2.509540e-03 2.116160 0.500 0.266 1.000000e+00
## FAM172A 2.519761e-03 1.044671 0.875 0.716 1.000000e+00
## AOPEP 2.557095e-03 1.244558 0.708 0.477 1.000000e+00
## ENSGALG00000052894 2.564357e-03 3.029827 0.125 0.009 1.000000e+00
## IRF7 2.564357e-03 2.676525 0.125 0.009 1.000000e+00
## ENSGALG00000046972 2.564357e-03 2.471121 0.125 0.009 1.000000e+00
## ENSGALG00000047688 2.564357e-03 1.395889 0.125 0.009 1.000000e+00
## ENSGALG00000051334 2.564357e-03 1.086334 0.125 0.009 1.000000e+00
## ARFGEF1 2.573665e-03 1.169892 0.833 0.569 1.000000e+00
## NT5C2 2.580560e-03 1.090828 0.750 0.541 1.000000e+00
## HS3ST5 2.607222e-03 1.253373 0.750 0.642 1.000000e+00
## HDAC4 2.614102e-03 1.415585 0.917 0.780 1.000000e+00
## ENSGALG00000001720 2.618604e-03 2.019274 0.458 0.202 1.000000e+00
## ENSGALG00000050037 2.627900e-03 3.332478 0.292 0.083 1.000000e+00
## TRPS1 2.631356e-03 1.828453 0.500 0.248 1.000000e+00
## ENSGALG00000047013 2.646334e-03 1.825283 0.333 0.110 1.000000e+00
## PATJ 2.648918e-03 1.139223 0.792 0.624 1.000000e+00
## EBF2 2.660258e-03 1.404867 0.958 0.963 1.000000e+00
## SNTG2 2.709410e-03 1.269741 0.542 0.294 1.000000e+00
## PDZRN3 2.718076e-03 1.577055 0.625 0.422 1.000000e+00
## ENSGALG00000047881 2.731722e-03 1.897014 0.917 0.853 1.000000e+00
## ENSGALG00000048902 2.736653e-03 1.890120 0.125 0.009 1.000000e+00
## SNX22 2.736653e-03 1.602355 0.125 0.009 1.000000e+00
## THAP9 2.736653e-03 1.595892 0.125 0.009 1.000000e+00
## CD3E 2.736653e-03 1.591301 0.125 0.009 1.000000e+00
## ENSGALG00000039896 2.736653e-03 1.406566 0.125 0.009 1.000000e+00
## ELP6 2.737660e-03 1.212902 0.583 0.294 1.000000e+00
## ATP5S 2.793181e-03 1.048566 0.542 0.239 1.000000e+00
## IFT88 2.854813e-03 1.142870 0.583 0.303 1.000000e+00
## APPL1 2.870157e-03 1.423782 0.500 0.229 1.000000e+00
## FAM161B 2.882384e-03 1.193206 0.375 0.138 1.000000e+00
## TPRG1L 2.887244e-03 -1.177670 0.667 0.899 1.000000e+00
## ENSGALG00000049634 2.919465e-03 2.015686 0.125 0.009 1.000000e+00
## ENSGALG00000020775 2.993913e-03 1.239175 0.417 0.156 1.000000e+00
## ENSGALG00000054524 3.035127e-03 -1.076922 0.167 0.532 1.000000e+00
## GRB10 3.061331e-03 2.095769 0.375 0.147 1.000000e+00
## ENSGALG00000033770 3.074592e-03 2.062453 0.667 0.477 1.000000e+00
## MICALL1 3.076033e-03 1.259203 0.417 0.156 1.000000e+00
## ITGA8 3.129447e-03 1.140761 0.792 0.606 1.000000e+00
## SMYD5 3.201762e-03 1.306010 0.375 0.128 1.000000e+00
## NADK2 3.218292e-03 1.818206 0.292 0.083 1.000000e+00
## TRAK1 3.252283e-03 1.355290 0.708 0.486 1.000000e+00
## CPNE3 3.290262e-03 1.708236 0.500 0.239 1.000000e+00
## CDYL2 3.327325e-03 1.438750 0.375 0.147 1.000000e+00
## ENSGALG00000051499 3.334193e-03 1.972611 0.667 0.459 1.000000e+00
## AMDHD2 3.349653e-03 -2.902495 0.000 0.284 1.000000e+00
## ENSGALG00000051537 3.446817e-03 2.117909 0.292 0.092 1.000000e+00
## GTF2H1 3.449755e-03 1.389599 0.500 0.257 1.000000e+00
## ENSGALG00000054301 3.457511e-03 4.068302 0.250 0.064 1.000000e+00
## ENSGALG00000049671 3.530527e-03 1.116639 0.708 0.431 1.000000e+00
## HIVEP3 3.578238e-03 1.600057 0.792 0.661 1.000000e+00
## DIS3 3.591230e-03 1.110894 0.333 0.110 1.000000e+00
## MTF2 3.592836e-03 1.403101 0.542 0.284 1.000000e+00
## PROSER1 3.659364e-03 1.349142 0.583 0.294 1.000000e+00
## DROSHA 3.686401e-03 1.585787 0.708 0.459 1.000000e+00
## UBXN2A 3.686750e-03 -1.182450 0.542 0.798 1.000000e+00
## SCNN1B 3.718281e-03 2.948158 0.250 0.064 1.000000e+00
## ATXN1 3.724658e-03 2.311239 0.500 0.266 1.000000e+00
## ENSGALG00000002671 3.747924e-03 1.577937 0.583 0.321 1.000000e+00
## ENSGALG00000012187 3.755903e-03 1.312802 0.833 0.688 1.000000e+00
## TTC28 3.775463e-03 1.452279 0.792 0.725 1.000000e+00
## DLGAP2 3.801266e-03 2.193453 0.417 0.193 1.000000e+00
## LRRC38 3.813600e-03 1.291708 0.333 0.110 1.000000e+00
## RYR3 3.817853e-03 1.257402 0.875 0.881 1.000000e+00
## TAB1 3.872389e-03 1.027972 0.708 0.495 1.000000e+00
## SH3GLB1 3.878117e-03 1.051813 0.708 0.413 1.000000e+00
## LGR4 3.931129e-03 2.286273 0.542 0.312 1.000000e+00
## ARHGAP6 3.986224e-03 1.181566 0.667 0.477 1.000000e+00
## EWSR1 4.005658e-03 2.388920 0.500 0.275 1.000000e+00
## CKLF 4.010029e-03 -1.485423 0.125 0.459 1.000000e+00
## ENSGALG00000031260 4.010483e-03 1.004975 0.625 0.376 1.000000e+00
## CNOT4 4.043466e-03 1.098973 0.750 0.477 1.000000e+00
## CYP27C1 4.066907e-03 2.957067 0.208 0.046 1.000000e+00
## ADCY1 4.079352e-03 1.319146 0.750 0.661 1.000000e+00
## XPNPEP1 4.080526e-03 1.561623 0.583 0.339 1.000000e+00
## ENSGALG00000052837 4.085196e-03 1.578949 0.333 0.119 1.000000e+00
## TIAM1 4.136779e-03 1.815170 0.625 0.495 1.000000e+00
## ENSGALG00000016646 4.159000e-03 1.618600 0.500 0.239 1.000000e+00
## EEF2K 4.179540e-03 1.639736 0.292 0.092 1.000000e+00
## DPP4 4.189592e-03 2.448739 0.500 0.275 1.000000e+00
## MAPK11 4.193359e-03 1.208134 0.292 0.083 1.000000e+00
## EPC1 4.234701e-03 2.918124 0.208 0.046 1.000000e+00
## ENSGALG00000048190 4.408750e-03 1.268358 0.208 0.046 1.000000e+00
## ENSGALG00000048526 4.457977e-03 2.250175 0.375 0.156 1.000000e+00
## PSEN1 4.466154e-03 1.509135 0.458 0.211 1.000000e+00
## CNTN2 4.477605e-03 1.303820 0.625 0.404 1.000000e+00
## ENSGALG00000052040 4.582121e-03 2.258462 0.167 0.028 1.000000e+00
## ENSGALG00000051348 4.589258e-03 1.841899 0.208 0.046 1.000000e+00
## CCP110 4.645933e-03 1.331289 0.500 0.220 1.000000e+00
## ENSGALG00000052349 4.660645e-03 -1.423686 0.042 0.349 1.000000e+00
## PBX1 4.665160e-03 1.373361 0.875 0.743 1.000000e+00
## PHACTR1 4.669944e-03 1.258217 0.958 0.963 1.000000e+00
## ENSGALG00000009608 4.674981e-03 1.095555 0.708 0.450 1.000000e+00
## ENSGALG00000047468 4.693923e-03 1.433960 0.333 0.110 1.000000e+00
## NRP1 4.740382e-03 2.677651 0.375 0.147 1.000000e+00
## EDRF1 4.777878e-03 2.170500 0.250 0.064 1.000000e+00
## CDKAL1 4.815835e-03 1.119242 0.750 0.541 1.000000e+00
## PIGF 4.826525e-03 -1.084339 0.417 0.734 1.000000e+00
## GPC6 4.931704e-03 1.485434 0.292 0.083 1.000000e+00
## GNPTAB 4.935289e-03 1.149083 0.750 0.486 1.000000e+00
## ADCY2 4.941603e-03 1.002808 0.875 0.807 1.000000e+00
## MITF 4.951867e-03 1.143775 0.708 0.459 1.000000e+00
## PDE3A 4.974674e-03 -1.235992 0.458 0.202 1.000000e+00
## ZNF592 4.991397e-03 1.038600 0.583 0.330 1.000000e+00
## ANKRD29 4.995341e-03 1.359000 0.417 0.193 1.000000e+00
## VNN1 5.034923e-03 1.761017 0.167 0.028 1.000000e+00
## ENSGALG00000053721 5.034923e-03 1.362063 0.167 0.028 1.000000e+00
## ELAC2 5.072218e-03 1.455179 0.458 0.220 1.000000e+00
## IKZF5 5.211055e-03 -1.332923 0.292 0.679 1.000000e+00
## CACNG4 5.218345e-03 1.060235 0.458 0.202 1.000000e+00
## CDCP1 5.310401e-03 1.016600 0.250 0.064 1.000000e+00
## ENSGALG00000053332 5.380139e-03 1.868188 0.208 0.046 1.000000e+00
## E2F5 5.380139e-03 1.609862 0.208 0.046 1.000000e+00
## ENSGALG00000050842 5.380139e-03 1.336459 0.208 0.046 1.000000e+00
## PPP1R9A 5.397418e-03 1.302750 0.708 0.615 1.000000e+00
## HECW1 5.449865e-03 1.441354 0.833 0.670 1.000000e+00
## FGF13 5.509868e-03 1.010618 0.875 0.807 1.000000e+00
## ENSGALG00000050673 5.583893e-03 1.674075 0.500 0.275 1.000000e+00
## ALG6 5.600865e-03 1.659975 0.417 0.183 1.000000e+00
## PGBD5 5.642747e-03 1.619075 0.417 0.193 1.000000e+00
## TBC1D32 5.665395e-03 1.370167 0.625 0.376 1.000000e+00
## MTA3 5.697922e-03 1.446150 0.500 0.266 1.000000e+00
## C1D 5.699214e-03 -1.800550 0.333 0.615 1.000000e+00
## DARS 5.765493e-03 1.029749 0.625 0.431 1.000000e+00
## ENSGALG00000034572 5.780970e-03 2.146640 0.417 0.193 1.000000e+00
## CNTN6 5.799272e-03 2.100252 0.667 0.486 1.000000e+00
## MED13L 5.836324e-03 2.244227 0.917 0.844 1.000000e+00
## CEP164 5.858830e-03 1.179595 0.583 0.413 1.000000e+00
## TRMT61B 5.860371e-03 1.236187 0.375 0.147 1.000000e+00
## NAB2 6.001259e-03 2.318277 0.375 0.165 1.000000e+00
## ENSGALG00000009468 6.027312e-03 2.117525 0.417 0.183 1.000000e+00
## LEMD3 6.058872e-03 1.241423 0.667 0.468 1.000000e+00
## PSMD5 6.093206e-03 -1.339532 0.583 0.798 1.000000e+00
## ENSGALG00000049289 6.198164e-03 1.825061 0.708 0.532 1.000000e+00
## RAPGEF4 6.289983e-03 1.305068 0.875 0.872 1.000000e+00
## SFRP4 6.292095e-03 1.364089 0.208 0.046 1.000000e+00
## ENSGALG00000050152 6.341363e-03 2.480472 0.250 0.073 1.000000e+00
## ARID2 6.348059e-03 1.301877 0.792 0.697 1.000000e+00
## ENSGALG00000054848 6.358741e-03 1.717580 0.625 0.394 1.000000e+00
## ASCC3 6.393410e-03 1.249263 0.583 0.330 1.000000e+00
## GAB3 6.406871e-03 2.608790 0.333 0.128 1.000000e+00
## COL4A2 6.449377e-03 1.726286 0.333 0.119 1.000000e+00
## TASOR 6.477225e-03 1.609595 0.500 0.257 1.000000e+00
## SMAD9 6.555821e-03 1.699353 0.250 0.073 1.000000e+00
## AHDC1 6.612220e-03 1.535800 0.625 0.394 1.000000e+00
## ENSGALG00000048321 6.637760e-03 1.047934 0.500 0.275 1.000000e+00
## CADPS2 6.730325e-03 -1.190401 0.708 0.807 1.000000e+00
## PPTC7 6.813585e-03 1.141375 0.458 0.211 1.000000e+00
## CPEB4 6.821280e-03 2.155582 0.500 0.330 1.000000e+00
## CDH18 6.853314e-03 1.754141 0.667 0.569 1.000000e+00
## ENSGALG00000054235 6.861082e-03 1.643205 0.333 0.138 1.000000e+00
## ENSGALG00000000264 6.927723e-03 1.280162 0.500 0.266 1.000000e+00
## MCC 6.948742e-03 1.165544 0.875 0.670 1.000000e+00
## MAPKAPK5 7.001393e-03 2.019742 0.417 0.193 1.000000e+00
## ACP6 7.004407e-03 2.643954 0.250 0.073 1.000000e+00
## MARCHF4 7.076951e-03 1.529109 0.500 0.275 1.000000e+00
## TMEM266 7.117684e-03 1.735936 0.667 0.541 1.000000e+00
## TLN1 7.133327e-03 1.288076 0.458 0.211 1.000000e+00
## ENSGALG00000049127 7.250320e-03 -1.371531 0.333 0.624 1.000000e+00
## FNDC3A 7.294298e-03 1.610093 0.792 0.624 1.000000e+00
## ENSGALG00000041838 7.302081e-03 2.066962 0.292 0.092 1.000000e+00
## PHF12 7.312846e-03 1.603038 0.708 0.550 1.000000e+00
## ENSGALG00000048004 7.366874e-03 2.739091 0.500 0.284 1.000000e+00
## PCGF3 7.450367e-03 1.215846 0.708 0.532 1.000000e+00
## RAB3C 7.491491e-03 1.010792 0.750 0.560 1.000000e+00
## RASA2 7.495272e-03 1.719066 0.458 0.220 1.000000e+00
## ENSGALG00000015219 7.520315e-03 2.129017 0.417 0.202 1.000000e+00
## PTK7 7.668629e-03 1.597232 0.375 0.156 1.000000e+00
## THNSL1 7.694471e-03 1.358987 0.417 0.193 1.000000e+00
## RMDN3 7.855815e-03 1.981098 0.417 0.211 1.000000e+00
## CNNM2 7.856011e-03 1.206667 0.875 0.679 1.000000e+00
## NOS3 7.875059e-03 1.023511 0.500 0.266 1.000000e+00
## RPS6KA3 8.013211e-03 1.701487 0.667 0.697 1.000000e+00
## CNTN3 8.094662e-03 1.304822 0.542 0.275 1.000000e+00
## SYT14 8.129333e-03 2.850323 0.458 0.275 1.000000e+00
## ZC3H3 8.141298e-03 1.521239 0.917 0.835 1.000000e+00
## RALGPS1 8.148375e-03 2.275432 0.542 0.367 1.000000e+00
## NOL9 8.198493e-03 1.613213 0.458 0.220 1.000000e+00
## ENSGALG00000049876 8.241631e-03 1.467153 0.292 0.101 1.000000e+00
## SEMA5B 8.256239e-03 1.799510 0.500 0.303 1.000000e+00
## EI24 8.315257e-03 -2.835907 0.208 0.459 1.000000e+00
## TMEM255B 8.593652e-03 2.010983 0.542 0.349 1.000000e+00
## MPP7 8.610681e-03 1.212453 0.708 0.532 1.000000e+00
## CA6 8.835058e-03 2.628899 0.375 0.193 1.000000e+00
## SPATA5 8.843694e-03 2.514921 0.458 0.275 1.000000e+00
## ENSGALG00000049579 8.871525e-03 -1.028275 0.125 0.413 1.000000e+00
## PDE7B 8.906692e-03 1.200481 0.375 0.156 1.000000e+00
## BCKDHB 8.906874e-03 1.510384 0.625 0.468 1.000000e+00
## NDST3 8.929869e-03 1.811189 0.333 0.138 1.000000e+00
## SLC2A13 8.977738e-03 2.878697 0.750 0.670 1.000000e+00
## NAF1 9.129564e-03 1.584673 0.375 0.156 1.000000e+00
## TRAFD1 9.129564e-03 1.265177 0.375 0.156 1.000000e+00
## ENSGALG00000005180 9.211493e-03 1.030538 0.458 0.257 1.000000e+00
## MGRN1 9.244865e-03 1.037770 0.583 0.358 1.000000e+00
## ENSGALG00000050395 9.277846e-03 2.228842 0.458 0.248 1.000000e+00
## PTBP3 9.322812e-03 2.328850 0.500 0.257 1.000000e+00
## FGGY 9.364420e-03 1.124583 0.792 0.550 1.000000e+00
## MIB1 9.387538e-03 1.727784 0.542 0.330 1.000000e+00
## KLB 9.428967e-03 1.984142 0.292 0.110 1.000000e+00
## CCNDBP1 9.446812e-03 1.024790 0.833 0.633 1.000000e+00
## AMPD3 9.451538e-03 1.628119 0.500 0.284 1.000000e+00
## ZRSR2 9.519596e-03 2.061213 0.292 0.101 1.000000e+00
## GANC 9.650925e-03 1.896379 0.333 0.138 1.000000e+00
## NBN 9.677862e-03 -1.004740 0.500 0.211 1.000000e+00
## PLEKHG1 9.836813e-03 1.211138 0.792 0.624 1.000000e+00
## RAB18 9.894084e-03 -1.263713 0.583 0.789 1.000000e+00
## IMPA2 9.902562e-03 2.217199 0.333 0.138 1.000000e+00
## BBS9 9.910887e-03 1.373639 0.667 0.486 1.000000e+00
## TAMM41 9.991342e-03 -2.001198 0.125 0.413 1.000000e+00
## ENSGALG00000047690 1.007839e-02 1.798224 0.292 0.101 1.000000e+00
## DZIP1 1.007839e-02 1.400699 0.292 0.101 1.000000e+00
## LMNB1 1.007839e-02 1.350178 0.292 0.101 1.000000e+00
## ENSGALG00000048438 1.008935e-02 2.545576 0.250 0.083 1.000000e+00
## ENSGALG00000034806 1.010515e-02 3.065341 0.208 0.055 1.000000e+00
## ENSGALG00000047323 1.014243e-02 -1.686079 0.000 0.229 1.000000e+00
## RASL10B 1.014243e-02 -2.245749 0.000 0.229 1.000000e+00
## SPRED1 1.036287e-02 1.441585 0.667 0.468 1.000000e+00
## ENSGALG00000015435 1.036409e-02 1.533091 0.333 0.128 1.000000e+00
## RBP1 1.040417e-02 2.001438 0.250 0.083 1.000000e+00
## MALT1 1.044417e-02 1.935331 0.625 0.394 1.000000e+00
## PACSIN3 1.046539e-02 1.339908 0.500 0.284 1.000000e+00
## SH3BP4 1.046837e-02 2.746151 0.208 0.055 1.000000e+00
## EED 1.053078e-02 1.107052 0.417 0.211 1.000000e+00
## ENSGALG00000049699 1.058144e-02 1.714449 0.667 0.394 1.000000e+00
## GTSF1 1.062947e-02 -1.040623 0.750 0.844 1.000000e+00
## UBA6 1.069760e-02 1.951665 0.333 0.147 1.000000e+00
## PARD3B 1.081059e-02 1.913656 0.958 0.908 1.000000e+00
## PARP8 1.083362e-02 2.405269 0.292 0.110 1.000000e+00
## PDCL 1.097498e-02 -1.136300 0.167 0.459 1.000000e+00
## EIF1AX 1.099293e-02 -1.084173 0.792 0.807 1.000000e+00
## NXNL2 1.099508e-02 1.570169 0.250 0.073 1.000000e+00
## ENSGALG00000005020 1.106016e-02 1.306632 0.250 0.083 1.000000e+00
## XDH 1.109944e-02 1.103791 0.375 0.165 1.000000e+00
## TMTC2 1.113471e-02 1.032563 0.583 0.385 1.000000e+00
## ENSGALG00000007976 1.117181e-02 2.356495 0.500 0.303 1.000000e+00
## SFXN5 1.124234e-02 1.145625 0.458 0.266 1.000000e+00
## ENSGALG00000054900 1.128433e-02 1.408758 0.292 0.101 1.000000e+00
## ENSGALG00000036204 1.132363e-02 2.041099 0.667 0.440 1.000000e+00
## BRD8 1.137848e-02 1.331269 0.583 0.339 1.000000e+00
## ENSGALG00000041558 1.154408e-02 1.889831 0.458 0.239 1.000000e+00
## DGKD 1.159985e-02 2.423534 0.458 0.257 1.000000e+00
## TDRD1 1.169802e-02 2.590911 0.125 0.018 1.000000e+00
## ENSGALG00000028457 1.169802e-02 2.316660 0.125 0.018 1.000000e+00
## ENSGALG00000048224 1.169802e-02 1.709815 0.125 0.018 1.000000e+00
## TCERG1L 1.172042e-02 1.073839 0.500 0.248 1.000000e+00
## DENND6A 1.177607e-02 1.755095 0.375 0.183 1.000000e+00
## DEF8 1.181843e-02 1.739066 0.333 0.147 1.000000e+00
## TEAD4 1.189817e-02 1.347816 0.417 0.193 1.000000e+00
## PLEKHA8 1.192229e-02 1.792670 0.417 0.183 1.000000e+00
## CACNG2 1.194545e-02 1.249024 0.625 0.495 1.000000e+00
## CFAP57 1.199220e-02 1.090534 0.583 0.385 1.000000e+00
## CDCA9 1.204009e-02 1.129354 0.208 0.055 1.000000e+00
## ENSGALG00000050914 1.221303e-02 2.664939 0.167 0.037 1.000000e+00
## MPZ 1.221303e-02 1.676288 0.167 0.037 1.000000e+00
## XKR5 1.230166e-02 1.703230 0.125 0.018 1.000000e+00
## ENSGALG00000053867 1.230166e-02 1.608780 0.125 0.018 1.000000e+00
## ENSGALG00000042678 1.230166e-02 1.060494 0.125 0.018 1.000000e+00
## ENSGALG00000028207 1.230166e-02 1.043540 0.125 0.018 1.000000e+00
## ENSGALG00000054726 1.230166e-02 1.034469 0.125 0.018 1.000000e+00
## NPC1 1.241427e-02 1.622211 0.333 0.128 1.000000e+00
## ENSGALG00000052151 1.248330e-02 1.698546 0.250 0.083 1.000000e+00
## BFAR 1.251432e-02 1.156574 0.375 0.156 1.000000e+00
## ENSGALG00000029316 1.261625e-02 1.512515 0.583 0.422 1.000000e+00
## ENSGALG00000016441 1.271224e-02 2.533134 0.167 0.037 1.000000e+00
## ENSGALG00000048945 1.271224e-02 1.365794 0.167 0.037 1.000000e+00
## FANCI 1.272023e-02 1.723098 0.375 0.174 1.000000e+00
## HNF1A 1.272618e-02 2.986897 0.333 0.147 1.000000e+00
## FDXR 1.276248e-02 1.059640 0.292 0.110 1.000000e+00
## ENSGALG00000006864 1.276319e-02 1.315131 0.625 0.468 1.000000e+00
## ENSGALG00000009272 1.283013e-02 1.588042 0.417 0.211 1.000000e+00
## MYO1D 1.286349e-02 1.519655 0.250 0.083 1.000000e+00
## ECE1 1.290174e-02 1.560083 0.208 0.055 1.000000e+00
## ENSGALG00000054496 1.290174e-02 1.408476 0.208 0.055 1.000000e+00
## ENSGALG00000045115 1.293273e-02 2.213803 0.125 0.018 1.000000e+00
## KIF26B 1.309192e-02 1.260146 0.833 0.798 1.000000e+00
## ENSGALG00000034376 1.312185e-02 1.805122 0.458 0.257 1.000000e+00
## LRCH2 1.318769e-02 1.837777 0.708 0.486 1.000000e+00
## ENSGALG00000051377 1.322943e-02 1.502293 0.167 0.037 1.000000e+00
## RAF1 1.328652e-02 1.658776 0.792 0.761 1.000000e+00
## CPT1A 1.329226e-02 1.417133 0.625 0.404 1.000000e+00
## ENSGALG00000047383 1.335269e-02 1.392882 0.208 0.055 1.000000e+00
## ENSGALG00000035605 1.341076e-02 1.286648 0.333 0.138 1.000000e+00
## ARHGAP29 1.351224e-02 1.198589 0.417 0.202 1.000000e+00
## ENSGALG00000052009 1.359227e-02 1.744709 0.125 0.018 1.000000e+00
## ENSGALG00000052444 1.359227e-02 1.000459 0.125 0.018 1.000000e+00
## ENSGALG00000049095 1.359239e-02 1.027515 0.500 0.303 1.000000e+00
## ENSGALG00000035293 1.365475e-02 1.386741 0.250 0.083 1.000000e+00
## TNR 1.371565e-02 1.177230 0.542 0.339 1.000000e+00
## KLHL32 1.374833e-02 1.593502 0.542 0.358 1.000000e+00
## ZDHHC5 1.376513e-02 1.008755 0.167 0.037 1.000000e+00
## NRXN3 1.376872e-02 1.064223 0.958 0.972 1.000000e+00
## SNAPC5 1.399579e-02 -1.219525 0.458 0.633 1.000000e+00
## ENSGALG00000049098 1.443917e-02 1.467295 0.750 0.606 1.000000e+00
## TP63 1.444368e-02 3.009486 0.333 0.138 1.000000e+00
## SLC45A4 1.459590e-02 2.554121 0.292 0.110 1.000000e+00
## ASPG 1.466708e-02 1.557974 0.625 0.541 1.000000e+00
## CIB2 1.474129e-02 1.756427 0.417 0.220 1.000000e+00
## ENSGALG00000053609 1.474263e-02 -1.665975 0.167 0.413 1.000000e+00
## GOLIM4 1.479022e-02 2.172956 0.208 0.055 1.000000e+00
## ENSGALG00000052328 1.492226e-02 1.901475 0.250 0.083 1.000000e+00
## WDR7 1.511968e-02 1.253617 0.833 0.789 1.000000e+00
## CSTF3 1.522878e-02 1.371543 0.458 0.266 1.000000e+00
## PGPEP1L 1.523386e-02 1.201471 0.458 0.229 1.000000e+00
## BCO2 1.525906e-02 -1.194559 0.167 0.468 1.000000e+00
## HGSNAT 1.536723e-02 1.305546 0.250 0.083 1.000000e+00
## SMOX 1.539195e-02 1.213587 0.292 0.110 1.000000e+00
## TSNAX 1.545249e-02 1.916356 0.375 0.183 1.000000e+00
## ZBTB41 1.548890e-02 1.955769 0.167 0.037 1.000000e+00
## CABIN1 1.579073e-02 1.168984 0.750 0.560 1.000000e+00
## PKN2 1.583028e-02 2.011836 0.333 0.147 1.000000e+00
## CAPN15 1.594971e-02 1.225215 0.667 0.505 1.000000e+00
## NMT2 1.599520e-02 1.097494 0.917 0.890 1.000000e+00
## ENSGALG00000049753 1.610430e-02 1.660003 0.167 0.037 1.000000e+00
## GLCE 1.622015e-02 -1.059474 0.208 0.431 1.000000e+00
## NOXA1 1.623931e-02 1.465189 0.458 0.248 1.000000e+00
## KSR2 1.639115e-02 1.629582 0.625 0.450 1.000000e+00
## PCDH15 1.651867e-02 3.153327 0.250 0.092 1.000000e+00
## ENSGALG00000045754 1.655767e-02 1.208887 0.833 0.706 1.000000e+00
## TNFRSF4 1.658753e-02 2.854744 0.208 0.064 1.000000e+00
## ENSGALG00000023439 1.658753e-02 1.914597 0.208 0.064 1.000000e+00
## ENSGALG00000048972 1.662622e-02 1.248975 0.667 0.468 1.000000e+00
## ENSGALG00000054051 1.665813e-02 1.367557 0.667 0.606 1.000000e+00
## CWF19L2 1.671140e-02 1.146661 0.375 0.174 1.000000e+00
## CRY2 1.672199e-02 1.376094 0.333 0.138 1.000000e+00
## MPP4 1.681769e-02 3.140536 0.333 0.156 1.000000e+00
## ENSGALG00000035912 1.685035e-02 1.333861 0.792 0.661 1.000000e+00
## CHEK2 1.688507e-02 1.743422 0.375 0.183 1.000000e+00
## LRRC1 1.697427e-02 1.266373 0.667 0.560 1.000000e+00
## FAM171A1 1.701252e-02 1.528832 0.708 0.541 1.000000e+00
## MOB1B 1.726824e-02 1.367535 0.458 0.284 1.000000e+00
## ENSGALG00000014262 1.737032e-02 1.243978 0.750 0.624 1.000000e+00
## CSTF2 1.738753e-02 1.153474 0.500 0.266 1.000000e+00
## ACACA 1.744487e-02 1.277435 0.917 0.826 1.000000e+00
## RNF165 1.745657e-02 1.133267 0.500 0.284 1.000000e+00
## ATG16L1 1.754861e-02 1.044350 0.333 0.138 1.000000e+00
## ABCC8 1.762175e-02 1.662001 0.583 0.404 1.000000e+00
## CNOT10 1.777673e-02 1.174136 0.458 0.239 1.000000e+00
## DYX1C1 1.786672e-02 1.106453 0.375 0.174 1.000000e+00
## PHTF2 1.794492e-02 1.075949 0.500 0.275 1.000000e+00
## ENSGALG00000030195 1.845636e-02 2.022376 0.333 0.156 1.000000e+00
## MARCH8 1.845722e-02 1.679253 0.625 0.486 1.000000e+00
## MMP16 1.856410e-02 2.479107 0.583 0.440 1.000000e+00
## GALNT2 1.860747e-02 1.471371 0.750 0.706 1.000000e+00
## DDX59 1.866676e-02 1.160100 0.458 0.248 1.000000e+00
## PRKAA2 1.884239e-02 2.712315 0.208 0.064 1.000000e+00
## ENSGALG00000053035 1.884239e-02 1.926772 0.208 0.064 1.000000e+00
## ITPKB 1.890108e-02 1.884503 0.292 0.119 1.000000e+00
## USP45 1.891885e-02 1.044608 0.583 0.422 1.000000e+00
## NCAPD3 1.925243e-02 1.381547 0.375 0.183 1.000000e+00
## BACH2 1.933198e-02 1.641666 0.458 0.275 1.000000e+00
## DAGLA 1.935897e-02 2.486411 0.375 0.193 1.000000e+00
## WDR18 1.938374e-02 1.388806 0.292 0.119 1.000000e+00
## MON1A 1.939174e-02 -1.202002 0.167 0.404 1.000000e+00
## ENSGALG00000053454 1.949322e-02 1.811834 0.500 0.303 1.000000e+00
## ENSGALG00000006137 1.953727e-02 1.846476 0.250 0.092 1.000000e+00
## GID8 1.970705e-02 -1.090353 0.583 0.734 1.000000e+00
## GAB2 1.971046e-02 1.314919 0.417 0.220 1.000000e+00
## ENSGALG00000034946 1.971046e-02 1.106332 0.417 0.220 1.000000e+00
## ENSGALG00000041052 1.985800e-02 1.544704 0.500 0.303 1.000000e+00
## ENSGALG00000005464 1.996954e-02 1.079342 0.708 0.606 1.000000e+00
## REXO5 2.004296e-02 1.526706 0.333 0.147 1.000000e+00
## SOX8 2.010359e-02 1.085523 0.375 0.183 1.000000e+00
## CAP1 2.037164e-02 -1.054261 0.458 0.679 1.000000e+00
## SNX4 2.041156e-02 1.511572 0.417 0.211 1.000000e+00
## NEU2 2.041156e-02 1.053482 0.417 0.211 1.000000e+00
## TCTN2 2.042717e-02 -1.011429 0.000 0.193 1.000000e+00
## ENSGALG00000047505 2.042717e-02 -1.515292 0.000 0.193 1.000000e+00
## ANKIB1 2.056548e-02 1.173225 0.750 0.587 1.000000e+00
## SIRT1 2.064539e-02 1.478977 0.250 0.092 1.000000e+00
## DNAJB5 2.101911e-02 1.136313 0.292 0.110 1.000000e+00
## KRTCAP3 2.103003e-02 1.127846 0.792 0.606 1.000000e+00
## LLGL2 2.123484e-02 2.519148 0.583 0.468 1.000000e+00
## SOS2 2.130348e-02 1.113640 0.792 0.780 1.000000e+00
## MYO9B 2.184842e-02 1.262890 0.625 0.505 1.000000e+00
## KLHDC1 2.190490e-02 1.230259 0.375 0.183 1.000000e+00
## FBXO33 2.193022e-02 1.153456 0.875 0.844 1.000000e+00
## PDE4B 2.221487e-02 1.281745 0.667 0.514 1.000000e+00
## ENSGALG00000038755 2.231372e-02 -2.561869 0.083 0.303 1.000000e+00
## SCFD2 2.235532e-02 1.994978 0.583 0.440 1.000000e+00
## ENSGALG00000053853 2.241027e-02 1.220978 0.250 0.092 1.000000e+00
## MAPK14 2.244931e-02 2.371328 0.375 0.211 1.000000e+00
## TMEM223 2.248574e-02 -1.196878 0.500 0.624 1.000000e+00
## IKBKE 2.272664e-02 1.765137 0.208 0.064 1.000000e+00
## ENSGALG00000050502 2.296048e-02 2.775721 0.292 0.138 1.000000e+00
## SESN1 2.316011e-02 2.267271 0.542 0.440 1.000000e+00
## ENSGALG00000050213 2.321502e-02 2.000841 0.417 0.229 1.000000e+00
## PEAK1 2.328876e-02 1.268071 0.500 0.294 1.000000e+00
## MPLKIP 2.334652e-02 -1.027817 0.375 0.183 1.000000e+00
## DIPK2A 2.334652e-02 -2.756947 0.042 0.257 1.000000e+00
## C8A 2.343753e-02 1.576868 0.208 0.064 1.000000e+00
## MAP3K21 2.357086e-02 2.092632 0.458 0.248 1.000000e+00
## ENSGALG00000054941 2.364816e-02 1.092755 0.292 0.119 1.000000e+00
## SMTNL2 2.390858e-02 1.041228 0.375 0.193 1.000000e+00
## PGM5 2.409336e-02 1.054099 0.792 0.578 1.000000e+00
## CCDC113 2.416764e-02 2.147125 0.208 0.064 1.000000e+00
## IRF6 2.416764e-02 1.901021 0.208 0.064 1.000000e+00
## ENSGALG00000047951 2.416764e-02 1.491523 0.208 0.064 1.000000e+00
## ENSGALG00000030000 2.416764e-02 1.299545 0.208 0.064 1.000000e+00
## ENSGALG00000055022 2.418923e-02 1.611333 0.250 0.101 1.000000e+00
## FUT10 2.423463e-02 -1.963149 0.000 0.183 1.000000e+00
## HMCN1 2.428188e-02 2.261310 0.542 0.385 1.000000e+00
## LAPTM5 2.440048e-02 2.894655 0.167 0.046 1.000000e+00
## ENSGALG00000054030 2.441087e-02 1.347011 0.375 0.193 1.000000e+00
## HMGN4 2.466637e-02 1.361418 0.458 0.266 1.000000e+00
## RAPGEF5 2.481136e-02 1.997038 0.500 0.367 1.000000e+00
## B3GAT1 2.487529e-02 -1.147284 0.167 0.431 1.000000e+00
## ENSGALG00000035807 2.497242e-02 1.011934 0.333 0.138 1.000000e+00
## DDIT4 2.510961e-02 -1.479241 0.333 0.532 1.000000e+00
## ENSGALG00000043256 2.522607e-02 1.898315 0.292 0.138 1.000000e+00
## ARHGAP12 2.534609e-02 1.041439 0.583 0.459 1.000000e+00
## FTO 2.535284e-02 1.358431 0.542 0.339 1.000000e+00
## PBX4 2.539635e-02 1.048410 0.375 0.183 1.000000e+00
## ENSGALG00000050596 2.564373e-02 1.459105 0.250 0.092 1.000000e+00
## ENSGALG00000052684 2.615589e-02 2.112785 0.167 0.046 1.000000e+00
## TSPAN9 2.620639e-02 1.632598 0.625 0.477 1.000000e+00
## ROR1 2.623873e-02 1.478370 0.500 0.321 1.000000e+00
## SIAH2 2.652182e-02 -1.181512 0.125 0.367 1.000000e+00
## SLC6A12 2.652806e-02 -1.459596 0.083 0.321 1.000000e+00
## RNF213 2.662297e-02 2.174489 0.417 0.229 1.000000e+00
## PTPRG 2.706246e-02 1.288353 0.917 0.872 1.000000e+00
## RAP1B 2.720043e-02 -1.291253 0.500 0.725 1.000000e+00
## MZT1 2.723965e-02 -1.005635 0.500 0.706 1.000000e+00
## INTS7 2.735982e-02 1.306689 0.292 0.119 1.000000e+00
## TNRC6B 2.770013e-02 1.103553 0.708 0.706 1.000000e+00
## CHSY3 2.776900e-02 1.560504 0.875 0.807 1.000000e+00
## ENSGALG00000053985 2.780226e-02 1.009612 0.708 0.440 1.000000e+00
## RUNX2 2.781893e-02 1.991327 0.583 0.422 1.000000e+00
## DPY19L4 2.787114e-02 2.376070 0.375 0.220 1.000000e+00
## TOR1A 2.787123e-02 -1.275707 0.250 0.477 1.000000e+00
## ENSGALG00000048977 2.801941e-02 1.439626 0.167 0.046 1.000000e+00
## ENSGALG00000044478 2.803229e-02 2.241945 0.375 0.211 1.000000e+00
## HEMK1 2.821092e-02 1.137869 0.417 0.229 1.000000e+00
## PLEKHA7 2.845191e-02 2.156887 0.625 0.495 1.000000e+00
## SLC16A4 2.870959e-02 -1.469381 0.000 0.174 1.000000e+00
## ENSGALG00000049872 2.870959e-02 -2.415885 0.000 0.174 1.000000e+00
## FXN 2.891443e-02 1.156897 0.333 0.156 1.000000e+00
## DUSP5 2.899334e-02 2.333074 0.167 0.046 1.000000e+00
## PANX3 2.899334e-02 2.036593 0.167 0.046 1.000000e+00
## ENSGALG00000038666 2.899334e-02 1.851944 0.167 0.046 1.000000e+00
## KIAA0907 2.901189e-02 1.989704 0.417 0.257 1.000000e+00
## CELF1 2.921246e-02 1.190959 0.542 0.385 1.000000e+00
## TRDMT1 2.927191e-02 1.307003 0.417 0.211 1.000000e+00
## ENSGALG00000009332 2.930003e-02 1.396203 0.625 0.477 1.000000e+00
## TRERF1 2.935806e-02 1.537024 0.375 0.193 1.000000e+00
## SUCLG2 2.936343e-02 1.941882 0.417 0.266 1.000000e+00
## NFAT5 2.938610e-02 1.410363 0.750 0.651 1.000000e+00
## ENSGALG00000054753 2.954346e-02 1.620385 0.333 0.165 1.000000e+00
## NEXMIF 2.955948e-02 1.796823 0.708 0.743 1.000000e+00
## ENSGALG00000020320 2.959088e-02 1.832507 0.292 0.128 1.000000e+00
## ANGEL2 2.974825e-02 1.595497 0.250 0.101 1.000000e+00
## ENSGALG00000039509 2.999627e-02 2.601842 0.167 0.046 1.000000e+00
## ENSGALG00000050578 3.010202e-02 1.543387 0.208 0.073 1.000000e+00
## GREB1 3.019593e-02 1.220572 0.542 0.385 1.000000e+00
## GNG13 3.021547e-02 -1.435404 0.125 0.339 1.000000e+00
## THADA 3.060724e-02 1.258351 0.458 0.294 1.000000e+00
## RECK 3.063315e-02 1.847125 0.500 0.385 1.000000e+00
## NEDD4L 3.075047e-02 1.217271 0.917 0.826 1.000000e+00
## ENSGALG00000050651 3.076941e-02 1.543538 0.458 0.266 1.000000e+00
## ENSGALG00000035275 3.121429e-02 1.354867 0.375 0.183 1.000000e+00
## SNAPC1 3.121429e-02 1.196853 0.375 0.183 1.000000e+00
## ENSGALG00000034725 3.123339e-02 1.101792 0.667 0.440 1.000000e+00
## ENSGALG00000016217 3.127755e-02 1.323294 0.750 0.761 1.000000e+00
## TMEM39B 3.165639e-02 1.219146 0.250 0.092 1.000000e+00
## FOXN3 3.177560e-02 2.008799 0.625 0.615 1.000000e+00
## FAM155B 3.187010e-02 1.765177 0.208 0.073 1.000000e+00
## ENSGALG00000047831 3.209183e-02 1.750925 0.167 0.046 1.000000e+00
## ENSGALG00000049505 3.209183e-02 1.136907 0.167 0.046 1.000000e+00
## ENSGALG00000049781 3.216505e-02 1.315628 0.333 0.165 1.000000e+00
## POR 3.217796e-02 1.070926 0.583 0.422 1.000000e+00
## ABHD4 3.227445e-02 1.026786 0.417 0.220 1.000000e+00
## GYG2 3.240152e-02 1.344280 0.125 0.028 1.000000e+00
## CDH8 3.246398e-02 1.508647 0.292 0.128 1.000000e+00
## ESYT2 3.269327e-02 2.399987 0.458 0.303 1.000000e+00
## AGO4 3.278995e-02 1.020841 0.500 0.303 1.000000e+00
## ZDHHC14 3.281191e-02 1.333827 0.542 0.394 1.000000e+00
## ENSGALG00000033780 3.281191e-02 -1.122905 0.208 0.468 1.000000e+00
## FGF20 3.318586e-02 1.245797 0.167 0.046 1.000000e+00
## ENSGALG00000049280 3.321851e-02 2.635665 0.292 0.128 1.000000e+00
## GRIP1 3.333720e-02 1.142631 0.708 0.615 1.000000e+00
## TMCC1 3.364878e-02 1.563084 0.375 0.202 1.000000e+00
## ENSGALG00000048636 3.374108e-02 1.632813 0.125 0.028 1.000000e+00
## ENSGALG00000015810 3.374108e-02 1.445389 0.125 0.028 1.000000e+00
## ENSGALG00000052998 3.374108e-02 1.371531 0.125 0.028 1.000000e+00
## ENSGALG00000045520 3.374108e-02 1.305004 0.125 0.028 1.000000e+00
## C5H11orf74 3.396444e-02 -1.667944 0.000 0.165 1.000000e+00
## NAPRT 3.396444e-02 -2.612718 0.000 0.165 1.000000e+00
## SNX13 3.403701e-02 1.487157 0.542 0.349 1.000000e+00
## NEK7 3.445683e-02 2.074665 0.375 0.193 1.000000e+00
## ENSGALG00000051022 3.448735e-02 1.309711 0.667 0.514 1.000000e+00
## CDKL1 3.460820e-02 1.338564 0.250 0.101 1.000000e+00
## TGFBRAP1 3.477269e-02 1.688266 0.292 0.128 1.000000e+00
## SEL1L3 3.493085e-02 1.117468 0.542 0.376 1.000000e+00
## KITLG 3.508407e-02 1.051073 0.333 0.147 1.000000e+00
## ENSGALG00000013437 3.512771e-02 1.405484 0.125 0.028 1.000000e+00
## AP1S3 3.512771e-02 1.265941 0.125 0.028 1.000000e+00
## ENSGALG00000033647 3.546990e-02 1.334765 0.167 0.046 1.000000e+00
## MAP3K5 3.550101e-02 1.759649 0.417 0.257 1.000000e+00
## NPL 3.556566e-02 -1.391717 0.208 0.477 1.000000e+00
## ENSGALG00000040433 3.557279e-02 1.070968 0.292 0.128 1.000000e+00
## TENM3 3.560210e-02 1.441190 0.750 0.596 1.000000e+00
## ENSGALG00000009809 3.578581e-02 1.111487 0.833 0.890 1.000000e+00
## ENSGALG00000054222 3.581358e-02 2.695992 0.667 0.606 1.000000e+00
## FOXK1 3.581358e-02 1.078097 0.750 0.587 1.000000e+00
## ENSGALG00000013268 3.634967e-02 1.980390 0.500 0.303 1.000000e+00
## CCND3 3.647011e-02 1.384832 0.333 0.165 1.000000e+00
## AGMAT 3.656270e-02 2.390582 0.125 0.028 1.000000e+00
## ENSGALG00000009292 3.656270e-02 2.106283 0.125 0.028 1.000000e+00
## MFSD9 3.656270e-02 2.074099 0.125 0.028 1.000000e+00
## MTNR1A 3.656270e-02 1.551743 0.125 0.028 1.000000e+00
## ENSGALG00000051260 3.656270e-02 1.105514 0.125 0.028 1.000000e+00
## ENSGALG00000017493 3.656270e-02 1.037352 0.125 0.028 1.000000e+00
## ENSGALG00000049394 3.656270e-02 -1.476633 0.125 0.028 1.000000e+00
## RAC1 3.721901e-02 1.783936 0.375 0.220 1.000000e+00
## LRRK2 3.728940e-02 1.902933 0.458 0.312 1.000000e+00
## PYGL 3.737898e-02 -1.389293 0.042 0.239 1.000000e+00
## ENSGALG00000029842 3.739371e-02 1.281734 0.375 0.183 1.000000e+00
## METTL8 3.747234e-02 1.320969 0.417 0.257 1.000000e+00
## MYL1 3.753171e-02 -2.472876 0.083 0.294 1.000000e+00
## NCOA5 3.753471e-02 1.220169 0.458 0.303 1.000000e+00
## ADGRL2 3.780410e-02 1.082027 0.833 0.789 1.000000e+00
## KDF1 3.804734e-02 2.116621 0.125 0.028 1.000000e+00
## RSAD2 3.804734e-02 1.159108 0.125 0.028 1.000000e+00
## ENSGALG00000004144 3.821220e-02 2.002359 0.250 0.101 1.000000e+00
## METTL9 3.821220e-02 1.043294 0.250 0.101 1.000000e+00
## ENSGALG00000027397 3.826826e-02 3.282248 0.375 0.211 1.000000e+00
## AGL 3.850274e-02 2.304399 0.417 0.266 1.000000e+00
## ENSGALG00000036259 3.857448e-02 2.026736 0.458 0.312 1.000000e+00
## KCTD18 3.877627e-02 1.291175 0.208 0.073 1.000000e+00
## CD226 3.883978e-02 1.082348 0.250 0.092 1.000000e+00
## ZFC3H1 3.891149e-02 1.036441 0.500 0.321 1.000000e+00
## ENSGALG00000012984 3.897280e-02 1.095609 0.333 0.156 1.000000e+00
## ZNF516 3.900201e-02 2.053047 0.375 0.211 1.000000e+00
## FLOT2 3.910377e-02 -2.039853 0.250 0.440 1.000000e+00
## KATNAL1 3.938941e-02 1.361577 0.458 0.275 1.000000e+00
## ENSGALG00000009387 3.956401e-02 1.058727 0.500 0.321 1.000000e+00
## GALNT16 4.012517e-02 1.659935 0.333 0.174 1.000000e+00
## ORC2 4.013079e-02 1.047376 0.250 0.101 1.000000e+00
## HCK 4.013079e-02 -1.915383 0.000 0.156 1.000000e+00
## ENSGALG00000054047 4.019257e-02 -1.552624 0.333 0.532 1.000000e+00
## PARVA 4.023359e-02 -1.860399 0.167 0.376 1.000000e+00
## ENSGALG00000047399 4.024508e-02 -2.631222 0.125 0.321 1.000000e+00
## DNAI1 4.024965e-02 1.209762 0.750 0.532 1.000000e+00
## TSC2 4.026318e-02 1.133397 0.917 0.936 1.000000e+00
## CDC25A 4.082658e-02 1.794338 0.375 0.202 1.000000e+00
## TMEM106C 4.087148e-02 -1.192909 0.208 0.431 1.000000e+00
## MED27 4.090841e-02 1.004325 0.833 0.725 1.000000e+00
## ENSGALG00000038416 4.096898e-02 1.098769 0.208 0.073 1.000000e+00
## PROS1 4.096898e-02 1.020583 0.208 0.073 1.000000e+00
## KAT2A 4.106124e-02 -1.774897 0.208 0.404 1.000000e+00
## ZPBP 4.117081e-02 1.472512 0.125 0.028 1.000000e+00
## GNGT2 4.117081e-02 1.356563 0.125 0.028 1.000000e+00
## MTG2 4.125929e-02 -1.626740 0.083 0.284 1.000000e+00
## CACTIN 4.156097e-02 1.170344 0.417 0.239 1.000000e+00
## CHSY1 4.166058e-02 1.396108 0.333 0.193 1.000000e+00
## PITPNM3 4.183539e-02 1.417439 0.708 0.569 1.000000e+00
## ENSGALG00000051715 4.205762e-02 2.330631 0.375 0.211 1.000000e+00
## KLHL8 4.270384e-02 2.370089 0.208 0.083 1.000000e+00
## ARF5 4.275345e-02 1.209674 0.458 0.284 1.000000e+00
## ENSGALG00000054114 4.281233e-02 -1.779510 0.125 0.028 1.000000e+00
## IGSF3 4.285242e-02 1.840298 0.375 0.211 1.000000e+00
## ENSGALG00000046863 4.287950e-02 1.990745 0.458 0.376 1.000000e+00
## ENSGALG00000051995 4.316655e-02 1.769788 0.250 0.110 1.000000e+00
## HEXD 4.318989e-02 1.558716 0.417 0.257 1.000000e+00
## ENSGALG00000048012 4.330138e-02 1.969019 0.500 0.349 1.000000e+00
## ST6GALNAC3 4.338298e-02 1.369293 0.792 0.633 1.000000e+00
## GRIK3 4.353464e-02 1.650465 0.417 0.266 1.000000e+00
## HAVCR1 4.356438e-02 -1.490092 0.250 0.477 1.000000e+00
## ANO1 4.365312e-02 2.123845 0.542 0.450 1.000000e+00
## IKBIP 4.391440e-02 -2.357856 0.125 0.330 1.000000e+00
## KLF12 4.399803e-02 1.329792 0.583 0.422 1.000000e+00
## AFG1L 4.400256e-02 1.095423 0.500 0.349 1.000000e+00
## TMEM68 4.419306e-02 2.584644 0.250 0.110 1.000000e+00
## PCSK1 4.434384e-02 2.231193 0.333 0.174 1.000000e+00
## ENSGALG00000012796 4.438395e-02 1.916917 0.500 0.385 1.000000e+00
## CC2D2A 4.473519e-02 1.147256 0.333 0.165 1.000000e+00
## ATP7A 4.483574e-02 1.458025 0.375 0.220 1.000000e+00
## CHFR 4.487731e-02 1.320781 0.375 0.202 1.000000e+00
## ENSGALG00000052075 4.500298e-02 1.959540 0.208 0.083 1.000000e+00
## EPGN 4.520634e-02 2.078396 0.167 0.055 1.000000e+00
## DMAP1 4.567573e-02 1.394950 0.667 0.523 1.000000e+00
## ENSGALG00000049348 4.587324e-02 -1.248316 0.417 0.560 1.000000e+00
## VWC2 4.613313e-02 1.331103 0.333 0.174 1.000000e+00
## HMBOX1 4.615976e-02 1.334915 0.375 0.211 1.000000e+00
## INTS6L 4.650913e-02 1.672043 0.333 0.183 1.000000e+00
## CPNE8 4.667258e-02 1.146574 0.792 0.872 1.000000e+00
## STK38L 4.688914e-02 1.532854 0.625 0.514 1.000000e+00
## EME2 4.689834e-02 1.061126 0.333 0.156 1.000000e+00
## MEGF9 4.736319e-02 -1.525777 0.000 0.147 1.000000e+00
## ENSGALG00000037186 4.739700e-02 1.560209 0.250 0.110 1.000000e+00
## ENSGALG00000050210 4.740564e-02 2.197108 0.208 0.083 1.000000e+00
## TMC6 4.740564e-02 1.208641 0.208 0.083 1.000000e+00
## UHRF1 4.741951e-02 1.241604 0.333 0.183 1.000000e+00
## CHCHD4 4.769692e-02 -2.107428 0.375 0.514 1.000000e+00
## ENSGALG00000054169 4.802632e-02 1.974849 0.167 0.055 1.000000e+00
## ENSGALG00000011285 4.827027e-02 -1.545735 0.167 0.376 1.000000e+00
## ENSGALG00000049811 4.850747e-02 1.059337 0.250 0.110 1.000000e+00
## TBCC 4.864685e-02 1.530789 0.208 0.083 1.000000e+00
## DDX52 4.892089e-02 1.049776 0.625 0.413 1.000000e+00
## ENSGALG00000055095 4.942808e-02 1.986412 0.333 0.165 1.000000e+00
## ENSGALG00000052453 4.967949e-02 -1.039924 0.083 0.275 1.000000e+00
## FAT3 5.009641e-02 1.014703 0.542 0.404 1.000000e+00
## CNNM1 5.025461e-02 1.021547 0.583 0.477 1.000000e+00
## MAK16 5.064530e-02 -2.099183 0.083 0.266 1.000000e+00
## NEMP1 5.099267e-02 2.002296 0.167 0.055 1.000000e+00
## ENSGALG00000046955 5.099267e-02 1.527239 0.167 0.055 1.000000e+00
## SNX30 5.121225e-02 1.497764 0.333 0.183 1.000000e+00
## ENSGALG00000042023 5.124283e-02 1.039025 0.458 0.257 1.000000e+00
## IRX2 5.134615e-02 1.239978 0.292 0.138 1.000000e+00
## ENSGALG00000035237 5.139026e-02 -1.160870 0.708 0.844 1.000000e+00
## PDLIM7 5.152609e-02 1.023494 0.250 0.119 1.000000e+00
## PIK3CG 5.156002e-02 1.233818 0.375 0.193 1.000000e+00
## ZNF292 5.179109e-02 1.111326 0.542 0.385 1.000000e+00
## TBC1D12 5.184744e-02 1.842967 0.292 0.147 1.000000e+00
## ENSGALG00000048996 5.197076e-02 1.300845 0.250 0.110 1.000000e+00
## RCL1 5.290965e-02 1.009448 0.375 0.183 1.000000e+00
## ENSGALG00000034412 5.292654e-02 1.593970 0.292 0.147 1.000000e+00
## KCNB1 5.300137e-02 1.029624 0.375 0.229 1.000000e+00
## TRAM1L1 5.320191e-02 1.366954 0.333 0.183 1.000000e+00
## ENSGALG00000013950 5.344657e-02 1.431671 0.500 0.339 1.000000e+00
## ENSGALG00000054309 5.350996e-02 -1.454866 0.083 0.266 1.000000e+00
## ENSGALG00000003387 5.354600e-02 1.394758 0.292 0.138 1.000000e+00
## SLC48A1 5.361853e-02 -1.763486 0.625 0.633 1.000000e+00
## CLK3 5.390963e-02 1.371482 0.333 0.174 1.000000e+00
## PCCA 5.397674e-02 1.162249 0.750 0.679 1.000000e+00
## DTD1 5.433156e-02 -1.086550 0.542 0.679 1.000000e+00
## RAB30 5.434607e-02 -1.101551 0.542 0.642 1.000000e+00
## EFCAB7 5.441615e-02 2.712251 0.333 0.193 1.000000e+00
## GEMIN8 5.450466e-02 2.044001 0.333 0.202 1.000000e+00
## KLHL41 5.466176e-02 1.352560 0.500 0.376 1.000000e+00
## MLLT3 5.482143e-02 1.152985 0.708 0.679 1.000000e+00
## GRIN3A 5.495453e-02 1.544417 0.333 0.174 1.000000e+00
## TEX10 5.507111e-02 1.250547 0.542 0.376 1.000000e+00
## PYM1 5.526938e-02 1.053257 0.208 0.083 1.000000e+00
## ANAPC16 5.531859e-02 -1.463177 0.292 0.477 1.000000e+00
## CHIC1 5.540412e-02 1.292029 0.500 0.358 1.000000e+00
## BICC1 5.544022e-02 1.077194 0.333 0.193 1.000000e+00
## TMEM260 5.577455e-02 1.982333 0.542 0.394 1.000000e+00
## KPNA2 5.584381e-02 -2.163343 0.000 0.138 1.000000e+00
## DESI2 5.584381e-02 -2.251199 0.000 0.138 1.000000e+00
## PRRG3 5.584381e-02 -2.549182 0.000 0.138 1.000000e+00
## MICAL2 5.590353e-02 1.560687 0.542 0.459 1.000000e+00
## KLHL4 5.627703e-02 1.057556 0.292 0.147 1.000000e+00
## GAD1 5.627703e-02 -3.744501 0.042 0.202 1.000000e+00
## SNAP47 5.656162e-02 2.847560 0.292 0.156 1.000000e+00
## RHOBTB1 5.707616e-02 -1.254131 0.125 0.349 1.000000e+00
## AMOT 5.724091e-02 1.297153 0.792 0.743 1.000000e+00
## ENSGALG00000051546 5.738675e-02 1.610395 0.167 0.055 1.000000e+00
## UNC5C 5.746480e-02 1.822867 0.542 0.394 1.000000e+00
## ENSGALG00000037464 5.786148e-02 1.950522 0.375 0.229 1.000000e+00
## AKR1E2 5.805974e-02 1.288194 0.375 0.202 1.000000e+00
## TTC26 5.807266e-02 1.223287 0.458 0.312 1.000000e+00
## SHARPIN 5.812102e-02 1.156178 0.208 0.083 1.000000e+00
## CYFIP1 5.821698e-02 1.248935 0.750 0.725 1.000000e+00
## PAQR3 5.908551e-02 1.729856 0.167 0.055 1.000000e+00
## NKAIN3 5.926222e-02 1.944223 0.667 0.670 1.000000e+00
## GRAMD1C 5.933464e-02 1.078781 0.500 0.312 1.000000e+00
## HACE1 5.973639e-02 1.537189 0.375 0.220 1.000000e+00
## STXBP3 5.980227e-02 1.115993 0.292 0.147 1.000000e+00
## MICU2 6.001271e-02 2.264974 0.583 0.596 1.000000e+00
## ENSGALG00000054854 6.027620e-02 -1.449497 0.125 0.339 1.000000e+00
## RUNDC3A 6.031294e-02 -1.063753 0.333 0.486 1.000000e+00
## DYNC1LI2 6.048872e-02 -1.204384 0.708 0.789 1.000000e+00
## ENSGALG00000010074 6.053605e-02 -1.108933 0.083 0.275 1.000000e+00
## C11H19orf12 6.062998e-02 -1.014031 0.500 0.642 1.000000e+00
## ALDH1A2 6.101730e-02 1.281504 0.292 0.147 1.000000e+00
## AAMDC 6.101730e-02 1.147759 0.292 0.147 1.000000e+00
## ELP2 6.121790e-02 1.133441 0.333 0.165 1.000000e+00
## GPM6B 6.152350e-02 1.437137 0.583 0.404 1.000000e+00
## HS2ST1 6.219409e-02 1.095247 0.625 0.431 1.000000e+00
## WASH1 6.233135e-02 1.227837 0.542 0.468 1.000000e+00
## FGFR1 6.264481e-02 1.004083 0.583 0.413 1.000000e+00
## ATG7 6.303792e-02 1.017013 0.833 0.743 1.000000e+00
## ERCC3 6.309017e-02 -1.922030 0.333 0.495 1.000000e+00
## DLG5 6.322249e-02 1.335986 0.625 0.450 1.000000e+00
## LBH 6.333997e-02 -1.460469 0.292 0.468 1.000000e+00
## TELO2 6.350874e-02 2.102778 0.292 0.147 1.000000e+00
## ALG3 6.350874e-02 1.570150 0.292 0.147 1.000000e+00
## TFPI2 6.444161e-02 -2.682541 0.042 0.193 1.000000e+00
## PAAF1 6.478563e-02 -1.550931 0.042 0.202 1.000000e+00
## ENSGALG00000006838 6.496704e-02 -1.216346 0.208 0.422 1.000000e+00
## ENSGALG00000053950 6.498872e-02 1.472065 0.500 0.367 1.000000e+00
## BMPR1A 6.499137e-02 1.027130 0.583 0.450 1.000000e+00
## AGK 6.526821e-02 -1.474130 0.292 0.495 1.000000e+00
## ALKBH2 6.533242e-02 1.266145 0.333 0.183 1.000000e+00
## SFI1 6.562471e-02 1.463358 0.708 0.651 1.000000e+00
## TRMT44 6.578839e-02 -1.501867 0.000 0.128 1.000000e+00
## MLC1 6.578839e-02 -1.959314 0.000 0.128 1.000000e+00
## CAMK1G 6.578839e-02 -2.485981 0.000 0.128 1.000000e+00
## ENSGALG00000053036 6.578839e-02 -2.659487 0.000 0.128 1.000000e+00
## KCTD7 6.614759e-02 -1.026646 0.250 0.477 1.000000e+00
## GRAMD4 6.663801e-02 1.270116 0.583 0.505 1.000000e+00
## ENSGALG00000054566 6.699761e-02 1.504911 0.250 0.119 1.000000e+00
## ENSGALG00000031336 6.709637e-02 -2.165659 0.042 0.193 1.000000e+00
## ENSGALG00000048663 6.725045e-02 2.402623 0.250 0.128 1.000000e+00
## ENSGALG00000049914 6.731873e-02 3.013719 0.125 0.037 1.000000e+00
## ENSGALG00000049375 6.731873e-02 2.320479 0.125 0.037 1.000000e+00
## DMB2 6.731873e-02 1.685100 0.125 0.037 1.000000e+00
## MYADML2 6.780188e-02 -1.624871 0.083 0.248 1.000000e+00
## GPLD1 6.844362e-02 1.325441 0.250 0.119 1.000000e+00
## ENSGALG00000010034 6.845713e-02 1.099409 0.292 0.138 1.000000e+00
## CREBL2 6.873918e-02 -1.084273 0.500 0.615 1.000000e+00
## MMAB 6.874395e-02 -1.489968 0.042 0.202 1.000000e+00
## FBRSL1 6.880789e-02 1.918472 0.458 0.349 1.000000e+00
## ENSGALG00000041435 6.908030e-02 1.144189 0.208 0.083 1.000000e+00
## ENSGALG00000048679 6.926478e-02 1.783339 0.583 0.413 1.000000e+00
## ENSGALG00000045251 6.945364e-02 1.006609 0.250 0.110 1.000000e+00
## SLC3A1 6.961309e-02 2.313030 0.125 0.037 1.000000e+00
## ENSGALG00000011609 6.961309e-02 1.661786 0.125 0.037 1.000000e+00
## POLE 6.978723e-02 1.362721 0.375 0.220 1.000000e+00
## EDA 7.025515e-02 1.193400 0.708 0.633 1.000000e+00
## ACSL5 7.101749e-02 1.632476 0.458 0.367 1.000000e+00
## RHOT2 7.105082e-02 1.031914 0.375 0.229 1.000000e+00
## ENSGALG00000009205 7.125537e-02 1.055793 0.708 0.615 1.000000e+00
## GUCD1 7.149164e-02 -1.105027 0.042 0.202 1.000000e+00
## DENND2C 7.155038e-02 1.944746 0.292 0.156 1.000000e+00
## SSBP3 7.168477e-02 1.453228 0.667 0.596 1.000000e+00
## PDE1A 7.187674e-02 2.434727 0.375 0.257 1.000000e+00
## PANK3 7.197127e-02 2.499598 0.125 0.037 1.000000e+00
## ERAP1 7.197127e-02 2.056883 0.125 0.037 1.000000e+00
## SMIM19 7.201563e-02 -1.207898 0.167 0.349 1.000000e+00
## ENSGALG00000050892 7.206946e-02 1.644519 0.458 0.367 1.000000e+00
## PIGC 7.250633e-02 1.063242 0.208 0.083 1.000000e+00
## LNX2 7.275527e-02 1.038733 0.417 0.257 1.000000e+00
## ENSGALG00000016285 7.293625e-02 -1.268007 0.250 0.119 1.000000e+00
## TAF4B 7.307505e-02 1.698582 0.250 0.128 1.000000e+00
## SAAL1 7.311105e-02 1.325066 0.500 0.367 1.000000e+00
## EPX 7.349563e-02 1.403891 0.208 0.092 1.000000e+00
## COA6 7.358591e-02 -1.185014 0.208 0.376 1.000000e+00
## ENSGALG00000033206 7.413561e-02 -1.262139 0.542 0.642 1.000000e+00
## TEK 7.427081e-02 -1.711650 0.208 0.083 1.000000e+00
## USP28 7.435003e-02 1.082185 0.583 0.505 1.000000e+00
## RSPH4A 7.439448e-02 1.602808 0.125 0.037 1.000000e+00
## ENSGALG00000054191 7.439448e-02 1.409702 0.125 0.037 1.000000e+00
## ADAMTS3 7.441273e-02 1.137484 0.833 0.780 1.000000e+00
## ENSGALG00000050823 7.472081e-02 1.666446 0.167 0.064 1.000000e+00
## EDC4 7.477691e-02 1.196211 0.458 0.294 1.000000e+00
## CDC40 7.489216e-02 1.496948 0.458 0.275 1.000000e+00
## NAAA 7.521746e-02 -2.216583 0.042 0.183 1.000000e+00
## ASXL2 7.571520e-02 1.338840 0.583 0.477 1.000000e+00
## MARCH1 7.587425e-02 1.029854 0.625 0.495 1.000000e+00
## ENSGALG00000041833 7.606012e-02 -1.351436 0.208 0.385 1.000000e+00
## NLGN1 7.660772e-02 1.565510 0.667 0.706 1.000000e+00
## ENSGALG00000007645 7.673952e-02 1.914167 0.167 0.064 1.000000e+00
## ENSGALG00000033819 7.688395e-02 2.774238 0.125 0.037 1.000000e+00
## C5orf34 7.688395e-02 1.766809 0.125 0.037 1.000000e+00
## ENSGALG00000054498 7.688395e-02 1.115410 0.125 0.037 1.000000e+00
## BCS1L 7.725709e-02 1.217732 0.292 0.147 1.000000e+00
## ENSGALG00000040646 7.745392e-02 -1.124781 0.000 0.119 1.000000e+00
## ENSGALG00000049910 7.745392e-02 -1.189840 0.000 0.119 1.000000e+00
## DUSP7 7.745392e-02 -1.961097 0.000 0.119 1.000000e+00
## ING2 7.745392e-02 -1.962347 0.000 0.119 1.000000e+00
## LRP3 7.745392e-02 -1.985810 0.000 0.119 1.000000e+00
## ENSGALG00000047879 7.745392e-02 -3.149772 0.000 0.119 1.000000e+00
## FHIT 7.764313e-02 1.052632 0.917 0.917 1.000000e+00
## C10orf2 7.770917e-02 1.745955 0.250 0.128 1.000000e+00
## ENSGALG00000016541 7.773993e-02 1.420683 0.417 0.266 1.000000e+00
## MTMR7 7.791772e-02 1.377529 0.500 0.404 1.000000e+00
## DNAJC28 7.792358e-02 -1.340332 0.125 0.312 1.000000e+00
## ENSGALG00000008309 7.814885e-02 -1.088712 0.083 0.248 1.000000e+00
## RAD54L 7.828929e-02 -1.970111 0.042 0.183 1.000000e+00
## YOD1 7.863000e-02 -1.767662 0.042 0.193 1.000000e+00
## SERAC1 7.880247e-02 1.885805 0.167 0.064 1.000000e+00
## FAM185A 7.890903e-02 2.141947 0.333 0.211 1.000000e+00
## CNTN4 7.912016e-02 1.503692 0.792 0.752 1.000000e+00
## IL1RAP 7.929888e-02 1.091121 0.250 0.119 1.000000e+00
## PLAG1 7.930605e-02 1.443203 0.250 0.128 1.000000e+00
## ENSGALG00000009650 7.946585e-02 1.808596 0.500 0.385 1.000000e+00
## NOP53 7.965782e-02 -1.762534 0.083 0.239 1.000000e+00
## RASAL2 7.992324e-02 1.374615 0.750 0.697 1.000000e+00
## SLX4 8.026429e-02 2.571923 0.333 0.211 1.000000e+00
## ENSGALG00000035137 8.039218e-02 1.413649 0.542 0.385 1.000000e+00
## SMIM14 8.077128e-02 1.642906 0.375 0.248 1.000000e+00
## CASP8 8.091032e-02 2.245251 0.167 0.064 1.000000e+00
## CD320 8.123022e-02 1.366911 0.625 0.532 1.000000e+00
## PNRC2 8.176322e-02 -1.257279 0.250 0.477 1.000000e+00
## ENSGALG00000047795 8.204911e-02 1.828251 0.333 0.202 1.000000e+00
## CTHRC1 8.206658e-02 1.056773 0.125 0.037 1.000000e+00
## ENSGALG00000053673 8.218093e-02 -1.025780 0.250 0.505 1.000000e+00
## TNRC6A 8.224098e-02 1.756233 0.542 0.450 1.000000e+00
## ACADSB 8.224098e-02 -1.313725 0.292 0.505 1.000000e+00
## CACNB4 8.230727e-02 1.373225 0.625 0.532 1.000000e+00
## AQR 8.232650e-02 1.081307 0.375 0.239 1.000000e+00
## ENSGALG00000047085 8.247998e-02 2.925344 0.208 0.092 1.000000e+00
## ENSGALG00000047110 8.247998e-02 1.537739 0.208 0.092 1.000000e+00
## ENSGALG00000047853 8.275698e-02 1.071814 0.625 0.459 1.000000e+00
## EFHC2 8.405477e-02 1.006411 0.542 0.413 1.000000e+00
## ENSGALG00000009341 8.409089e-02 1.347350 0.667 0.716 1.000000e+00
## ENSGALG00000049581 8.473924e-02 -2.088048 0.042 0.183 1.000000e+00
## CNOT6 8.505063e-02 1.364817 0.667 0.523 1.000000e+00
## DUS2 8.524457e-02 1.063808 0.333 0.193 1.000000e+00
## LEMD2 8.610568e-02 1.372660 0.250 0.119 1.000000e+00
## ENSGALG00000050964 8.631443e-02 1.284815 0.208 0.092 1.000000e+00
## UBTF 8.640783e-02 -2.118248 0.208 0.367 1.000000e+00
## KCNIP2 8.659580e-02 -1.277568 0.125 0.321 1.000000e+00
## CCDC84 8.750976e-02 1.183580 0.167 0.064 1.000000e+00
## ENSGALG00000049825 8.812181e-02 -2.126451 0.042 0.183 1.000000e+00
## KCNH5 8.914904e-02 2.101493 0.417 0.248 1.000000e+00
## LAMA5 8.919677e-02 1.889434 0.292 0.165 1.000000e+00
## EPB41L5 8.919677e-02 1.374228 0.292 0.165 1.000000e+00
## DIS3L 8.934491e-02 1.563804 0.417 0.284 1.000000e+00
## CD3D 8.980373e-02 2.368575 0.167 0.064 1.000000e+00
## MYSM1 8.980373e-02 1.089669 0.167 0.064 1.000000e+00
## PLA2G10 8.980373e-02 1.077394 0.167 0.064 1.000000e+00
## LSM10 8.980373e-02 1.001385 0.167 0.064 1.000000e+00
## NEU3 9.064181e-02 1.229917 0.375 0.239 1.000000e+00
## ENSGALG00000047393 9.070121e-02 -1.288858 0.167 0.339 1.000000e+00
## SOCS4 9.114879e-02 -1.105414 0.000 0.110 1.000000e+00
## PCTP 9.114879e-02 -1.249882 0.000 0.110 1.000000e+00
## BPIFB3 9.114879e-02 -1.483072 0.000 0.110 1.000000e+00
## DTYMK 9.114879e-02 -1.510473 0.000 0.110 1.000000e+00
## MPP5 9.114879e-02 -1.933461 0.000 0.110 1.000000e+00
## ENSGALG00000051025 9.114879e-02 -2.221267 0.000 0.110 1.000000e+00
## FXYD6 9.114879e-02 -2.307317 0.000 0.110 1.000000e+00
## ENSGALG00000035050 9.131006e-02 1.194084 0.292 0.156 1.000000e+00
## DISP1 9.140045e-02 1.411763 0.750 0.761 1.000000e+00
## LUC7L2 9.154277e-02 1.323964 0.583 0.459 1.000000e+00
## EXD1 9.161242e-02 -1.753910 0.042 0.183 1.000000e+00
## UFM1 9.162718e-02 -1.108721 0.500 0.615 1.000000e+00
## ENSGALG00000052870 9.165097e-02 2.748571 0.417 0.303 1.000000e+00
## ZNF827 9.165097e-02 1.305299 0.417 0.303 1.000000e+00
## GCNT7 9.209123e-02 -1.182752 0.125 0.294 1.000000e+00
## IFNAR2 9.219357e-02 1.187093 0.417 0.266 1.000000e+00
## CRY1 9.229383e-02 1.584716 0.417 0.321 1.000000e+00
## ENSGALG00000050487 9.233476e-02 1.726827 0.208 0.092 1.000000e+00
## TMEM70 9.260781e-02 -1.270279 0.417 0.560 1.000000e+00
## MIS18BP1 9.299604e-02 1.672126 0.292 0.156 1.000000e+00
## BNIP2 9.306301e-02 2.069449 0.250 0.128 1.000000e+00
## PHLPP1 9.313465e-02 1.310498 0.500 0.367 1.000000e+00
## TMEM161B 9.357624e-02 1.396625 0.417 0.275 1.000000e+00
## ABL2 9.394243e-02 -1.071824 0.083 0.257 1.000000e+00
## PLA2G7 9.396883e-02 1.293379 0.500 0.385 1.000000e+00
## FANCA 9.399316e-02 1.356037 0.583 0.459 1.000000e+00
## ENSGALG00000049133 9.415069e-02 1.085571 0.292 0.165 1.000000e+00
## TMEM123 9.441490e-02 -1.587872 0.208 0.092 1.000000e+00
## CFI 9.470691e-02 1.394029 0.292 0.156 1.000000e+00
## OPHN1 9.480419e-02 1.540807 0.333 0.183 1.000000e+00
## ZWILCH 9.499825e-02 2.441221 0.375 0.266 1.000000e+00
## MRPL58 9.575690e-02 -1.098972 0.583 0.642 1.000000e+00
## TIPRL 9.589788e-02 -1.488300 0.208 0.376 1.000000e+00
## NFIB 9.615642e-02 1.093762 0.625 0.532 1.000000e+00
## ENSGALG00000036550 9.643797e-02 -1.118205 0.083 0.239 1.000000e+00
## TMPRSS9 9.650796e-02 1.078864 0.208 0.101 1.000000e+00
## ANKRD22 9.668992e-02 -2.208534 0.083 0.229 1.000000e+00
## CD38 9.697721e-02 1.372471 0.167 0.064 1.000000e+00
## ENSGALG00000038978 9.858384e-02 1.372234 0.375 0.257 1.000000e+00
## ENSGALG00000050449 9.860210e-02 1.038474 0.208 0.101 1.000000e+00
## ENSGALG00000053335 9.868780e-02 1.234768 0.208 0.092 1.000000e+00
## ENSGALG00000049881 9.869145e-02 -1.241231 0.042 0.174 1.000000e+00
## ENSGALG00000048282 9.869666e-02 1.099645 0.375 0.275 1.000000e+00
## ENSGALG00000051889 9.933253e-02 -1.026997 0.125 0.037 1.000000e+00
## ENSGALG00000053961 1.000175e-01 1.865872 0.583 0.523 1.000000e+00
## MAVS 1.005597e-01 -1.371535 0.208 0.083 1.000000e+00
## PTPRT 1.006407e-01 1.432030 0.667 0.569 1.000000e+00
## VWC2L 1.007324e-01 1.659222 0.208 0.101 1.000000e+00
## ENSGALG00000048804 1.017868e-01 2.287410 0.250 0.138 1.000000e+00
## LRIG1 1.019888e-01 1.973349 0.292 0.174 1.000000e+00
## THOC1 1.020786e-01 1.223830 0.500 0.358 1.000000e+00
## NASP 1.024647e-01 1.253402 0.417 0.266 1.000000e+00
## ENSGALG00000049347 1.024853e-01 1.153883 0.542 0.440 1.000000e+00
## ZFYVE27 1.024853e-01 1.057262 0.542 0.440 1.000000e+00
## RIPPLY2 1.024886e-01 -1.596932 0.167 0.312 1.000000e+00
## ENSGALG00000040585 1.028992e-01 1.589839 0.208 0.101 1.000000e+00
## SEMA6D 1.029688e-01 1.048034 1.000 0.862 1.000000e+00
## ENSGALG00000027565 1.030859e-01 1.860687 0.417 0.349 1.000000e+00
## EIF4B 1.034884e-01 1.290873 0.500 0.358 1.000000e+00
## KATNBL1 1.034884e-01 1.260471 0.500 0.358 1.000000e+00
## DPH7 1.035032e-01 -1.450157 0.125 0.275 1.000000e+00
## RBM12B 1.044085e-01 1.222553 0.333 0.193 1.000000e+00
## ENSGALG00000005093 1.045884e-01 -1.611714 0.042 0.174 1.000000e+00
## ENSGALG00000050989 1.048894e-01 1.947779 0.417 0.294 1.000000e+00
## ENSGALG00000042748 1.053750e-01 1.860642 0.375 0.211 1.000000e+00
## SLITRK6 1.055047e-01 1.645448 0.167 0.073 1.000000e+00
## PRELID3B 1.059064e-01 -1.691968 0.417 0.541 1.000000e+00
## HHLA2 1.062352e-01 -2.317642 0.042 0.165 1.000000e+00
## TBL3 1.066151e-01 -2.236400 0.042 0.174 1.000000e+00
## DDHD1 1.070456e-01 1.962893 0.375 0.248 1.000000e+00
## ENSGALG00000046817 1.071802e-01 1.123233 0.333 0.202 1.000000e+00
## VAMP3 1.072465e-01 -1.066468 0.000 0.101 1.000000e+00
## ENSGALG00000031996 1.072465e-01 -1.067148 0.000 0.101 1.000000e+00
## ENSGALG00000031860 1.072465e-01 -1.072937 0.000 0.101 1.000000e+00
## KANK3 1.072465e-01 -1.094426 0.000 0.101 1.000000e+00
## ENSGALG00000045762 1.072465e-01 -1.224703 0.000 0.101 1.000000e+00
## ENSGALG00000006393 1.072465e-01 -1.272346 0.000 0.101 1.000000e+00
## OTOS 1.072465e-01 -1.404767 0.000 0.101 1.000000e+00
## EGFL7 1.072465e-01 -1.407485 0.000 0.101 1.000000e+00
## GNPDA1 1.072465e-01 -1.866460 0.000 0.101 1.000000e+00
## ENSGALG00000003836 1.072465e-01 -1.885044 0.000 0.101 1.000000e+00
## ENSGALG00000009128 1.072465e-01 -2.033157 0.000 0.101 1.000000e+00
## ENSGALG00000051396 1.072465e-01 -2.762326 0.000 0.101 1.000000e+00
## SCAMP4 1.074230e-01 -1.631631 0.417 0.596 1.000000e+00
## TPX2 1.079313e-01 1.201719 0.125 0.330 1.000000e+00
## ITGB5 1.083325e-01 -1.662496 0.042 0.165 1.000000e+00
## AKAP7 1.085586e-01 1.034518 0.417 0.275 1.000000e+00
## LGR5 1.095727e-01 1.159931 0.417 0.321 1.000000e+00
## NXPE3 1.095761e-01 -1.082651 0.500 0.587 1.000000e+00
## POGLUT3 1.096225e-01 1.724216 0.208 0.101 1.000000e+00
## ENSGALG00000054254 1.096225e-01 1.405582 0.208 0.101 1.000000e+00
## URB2 1.098056e-01 -1.510560 0.125 0.284 1.000000e+00
## PHEX 1.099361e-01 1.143585 0.625 0.514 1.000000e+00
## RASSF9 1.099440e-01 -1.955248 0.167 0.064 1.000000e+00
## PRLHR 1.107618e-01 2.756830 0.250 0.128 1.000000e+00
## GNG12 1.117195e-01 -1.417184 0.208 0.358 1.000000e+00
## SAMD12 1.120080e-01 1.153235 0.667 0.532 1.000000e+00
## PAXBP1 1.124801e-01 1.039114 0.792 0.789 1.000000e+00
## TGFB1I1 1.130323e-01 1.199363 0.208 0.422 1.000000e+00
## RNF126 1.135965e-01 -1.065172 0.208 0.385 1.000000e+00
## LSM14B 1.142277e-01 1.307728 0.292 0.165 1.000000e+00
## LMCD1 1.142944e-01 2.153167 0.208 0.101 1.000000e+00
## CCDC18 1.146004e-01 2.094502 0.250 0.147 1.000000e+00
## NAALAD2 1.160146e-01 2.339911 0.250 0.138 1.000000e+00
## PKDCC 1.160334e-01 1.765705 0.167 0.073 1.000000e+00
## ENSGALG00000050047 1.160334e-01 1.283157 0.167 0.073 1.000000e+00
## SLC22A15 1.161773e-01 1.341700 0.333 0.202 1.000000e+00
## ENSGALG00000050331 1.166884e-01 1.378949 0.208 0.101 1.000000e+00
## ZCCHC24 1.166921e-01 1.414549 0.708 0.587 1.000000e+00
## THOP1 1.167307e-01 1.401923 0.417 0.275 1.000000e+00
## PWP1 1.167560e-01 1.147672 0.375 0.229 1.000000e+00
## CYGB 1.170509e-01 -1.444198 0.042 0.165 1.000000e+00
## PUS3 1.170509e-01 -2.072488 0.042 0.165 1.000000e+00
## AP1G1 1.173092e-01 1.150988 0.542 0.413 1.000000e+00
## ENSGALG00000006345 1.191216e-01 1.504633 0.208 0.101 1.000000e+00
## ATCAY 1.194795e-01 -1.208712 0.542 0.633 1.000000e+00
## TSPAN5 1.199932e-01 1.433328 0.583 0.505 1.000000e+00
## ENSGALG00000009879 1.201562e-01 -1.142635 0.333 0.505 1.000000e+00
## ENSGALG00000054655 1.203585e-01 -1.546714 0.125 0.284 1.000000e+00
## SLC35C1 1.209552e-01 -1.488745 0.083 0.220 1.000000e+00
## ENSGALG00000048607 1.209682e-01 2.314655 0.250 0.147 1.000000e+00
## RIC8A 1.229934e-01 -2.633527 0.292 0.385 1.000000e+00
## RNF150 1.230159e-01 1.273061 0.583 0.459 1.000000e+00
## CYP2AB2 1.234454e-01 1.613968 0.125 0.046 1.000000e+00
## ASB16 1.234454e-01 1.135419 0.125 0.046 1.000000e+00
## PIK3C2A 1.234667e-01 1.185533 0.292 0.183 1.000000e+00
## ZNF341 1.241072e-01 1.245858 0.208 0.101 1.000000e+00
## C2CD2 1.250112e-01 -2.122012 0.250 0.367 1.000000e+00
## FOXK2 1.251547e-01 2.247578 0.333 0.220 1.000000e+00
## ENSGALG00000053170 1.252767e-01 2.648025 0.208 0.110 1.000000e+00
## LPL 1.259917e-01 -2.088004 0.042 0.156 1.000000e+00
## ENSGALG00000009935 1.263110e-01 -1.623958 0.042 0.165 1.000000e+00
## TTF1 1.265640e-01 -1.096258 0.125 0.275 1.000000e+00
## RNF8 1.266549e-01 -1.208232 0.625 0.734 1.000000e+00
## SEMA3E 1.266603e-01 -1.127621 0.208 0.101 1.000000e+00
## PLEKHM1 1.269417e-01 1.688267 0.125 0.046 1.000000e+00
## PDK4 1.269417e-01 1.292517 0.125 0.046 1.000000e+00
## POLG2 1.273708e-01 1.996634 0.167 0.073 1.000000e+00
## ZC3H12B 1.273708e-01 1.251411 0.167 0.073 1.000000e+00
## ENSGALG00000044466 1.282159e-01 -1.226898 0.208 0.394 1.000000e+00
## CD247 1.290437e-01 1.382558 0.333 0.220 1.000000e+00
## NEMP2 1.292541e-01 1.253039 0.208 0.101 1.000000e+00
## ENSGALG00000048914 1.294147e-01 1.303449 0.250 0.138 1.000000e+00
## RIC1 1.294641e-01 1.327917 0.333 0.229 1.000000e+00
## ENSGALG00000033453 1.303356e-01 1.037611 0.167 0.073 1.000000e+00
## ENSGALG00000054479 1.305146e-01 1.859827 0.125 0.046 1.000000e+00
## ENSGALG00000051651 1.305146e-01 1.676965 0.125 0.046 1.000000e+00
## ENSGALG00000052011 1.305146e-01 1.377482 0.125 0.046 1.000000e+00
## CENPS 1.308394e-01 1.199999 0.292 0.165 1.000000e+00
## ENSGALG00000013212 1.308563e-01 1.180638 0.375 0.239 1.000000e+00
## GJA1 1.319368e-01 -2.178026 0.083 0.211 1.000000e+00
## ARL14EP 1.324600e-01 -1.297822 0.292 0.404 1.000000e+00
## ODF2L 1.329061e-01 1.423911 0.208 0.110 1.000000e+00
## ENSGALG00000054205 1.329496e-01 1.069974 0.583 0.569 1.000000e+00
## SLC37A1 1.330259e-01 1.608617 0.333 0.220 1.000000e+00
## LOXL3 1.333537e-01 1.926245 0.167 0.073 1.000000e+00
## LRRN2 1.334639e-01 -1.731138 0.042 0.156 1.000000e+00
## CDH11 1.335963e-01 -2.713998 0.083 0.220 1.000000e+00
## FAM3C 1.340860e-01 -1.120404 0.625 0.697 1.000000e+00
## IFNGR1 1.341650e-01 1.634841 0.125 0.046 1.000000e+00
## ENSGALG00000052682 1.341650e-01 1.514438 0.125 0.046 1.000000e+00
## DTWD2 1.345313e-01 2.113502 0.250 0.147 1.000000e+00
## MINA 1.345351e-01 1.005737 0.333 0.211 1.000000e+00
## ENSGALG00000032359 1.345652e-01 1.950513 0.208 0.101 1.000000e+00
## ACAD11 1.345652e-01 1.717684 0.208 0.101 1.000000e+00
## ENSGALG00000027645 1.354175e-01 -1.626400 0.208 0.349 1.000000e+00
## SOGA1 1.354189e-01 1.522300 0.333 0.229 1.000000e+00
## GPN3 1.354297e-01 -1.236626 0.292 0.440 1.000000e+00
## ENSGALG00000053344 1.355276e-01 1.225652 0.208 0.110 1.000000e+00
## TULP4 1.355296e-01 1.881725 0.583 0.532 1.000000e+00
## ENSGALG00000054223 1.358052e-01 1.004894 0.500 0.339 1.000000e+00
## ENSGALG00000047256 1.360294e-01 -1.928057 0.042 0.156 1.000000e+00
## ENSGALG00000008988 1.361123e-01 2.092957 0.292 0.183 1.000000e+00
## ENSGALG00000028573 1.363979e-01 -1.013209 0.083 0.211 1.000000e+00
## ENSGALG00000053496 1.364256e-01 1.416490 0.167 0.073 1.000000e+00
## SNED1 1.364256e-01 1.021348 0.167 0.073 1.000000e+00
## ADNP2 1.365388e-01 1.562993 0.375 0.257 1.000000e+00
## CCDC112 1.378938e-01 1.239864 0.125 0.046 1.000000e+00
## ENSGALG00000049356 1.381889e-01 1.041022 0.208 0.110 1.000000e+00
## ENSGALG00000004946 1.383138e-01 1.962894 0.292 0.183 1.000000e+00
## ENSGALG00000051862 1.385007e-01 1.588019 0.375 0.257 1.000000e+00
## SLCO3A1 1.386968e-01 1.394485 0.333 0.211 1.000000e+00
## SPSB3 1.387830e-01 -2.147731 0.292 0.376 1.000000e+00
## SH3PXD2B 1.393046e-01 2.706160 0.250 0.147 1.000000e+00
## ENSGALG00000047593 1.394814e-01 1.478977 0.458 0.367 1.000000e+00
## CDK6 1.399010e-01 2.083123 0.500 0.440 1.000000e+00
## ENSGALG00000004477 1.405427e-01 1.071016 0.292 0.183 1.000000e+00
## ENSGALG00000001389 1.408814e-01 1.016693 0.917 0.761 1.000000e+00
## LIPT2 1.409745e-01 -1.740414 0.083 0.211 1.000000e+00
## GNB1L 1.409957e-01 1.161850 0.583 0.569 1.000000e+00
## LRP6 1.417816e-01 1.801326 0.333 0.248 1.000000e+00
## PDP1 1.424621e-01 -1.316151 0.625 0.725 1.000000e+00
## PURG 1.427330e-01 1.070546 0.167 0.073 1.000000e+00
## CAMLG 1.440376e-01 -1.911963 0.125 0.257 1.000000e+00
## GIGYF2 1.441272e-01 1.101279 0.667 0.606 1.000000e+00
## ANKRD16 1.442073e-01 1.458232 0.250 0.147 1.000000e+00
## EIF2AK2 1.451244e-01 1.418759 0.333 0.211 1.000000e+00
## MED7 1.455383e-01 -1.234683 0.167 0.303 1.000000e+00
## SGSM1 1.456919e-01 1.074574 0.208 0.101 1.000000e+00
## ENSGALG00000015418 1.461542e-01 1.266869 0.417 0.275 1.000000e+00
## DUPD1 1.465334e-01 1.935453 0.250 0.138 1.000000e+00
## RNF144A 1.471371e-01 2.128540 0.292 0.202 1.000000e+00
## ENSGALG00000006104 1.476946e-01 2.196620 0.167 0.083 1.000000e+00
## ENSGALG00000033254 1.476946e-01 1.140299 0.167 0.083 1.000000e+00
## ENSGALG00000032964 1.477157e-01 -1.030064 0.333 0.220 1.000000e+00
## ENSGALG00000053442 1.490877e-01 2.552664 0.250 0.156 1.000000e+00
## ENSGALG00000048471 1.492383e-01 1.940641 0.208 0.110 1.000000e+00
## TSR3 1.492383e-01 -2.815241 0.042 0.147 1.000000e+00
## EDARADD 1.494316e-01 -1.127742 0.042 0.156 1.000000e+00
## ENSGALG00000036877 1.495990e-01 -1.073936 0.250 0.413 1.000000e+00
## EPS8L3 1.496095e-01 1.056865 0.292 0.174 1.000000e+00
## ENSGALG00000051945 1.496628e-01 2.650349 0.292 0.193 1.000000e+00
## ENSGALG00000007516 1.500085e-01 1.449399 0.458 0.339 1.000000e+00
## CRTC1 1.500085e-01 1.265010 0.458 0.339 1.000000e+00
## FANCM 1.506401e-01 1.038674 0.500 0.431 1.000000e+00
## ENSGALG00000021686 1.506530e-01 -1.273960 0.125 0.257 1.000000e+00
## FIGN 1.507070e-01 2.194365 0.500 0.404 1.000000e+00
## CEP68 1.517092e-01 -1.728144 0.125 0.239 1.000000e+00
## TMEM164 1.519967e-01 1.229439 0.292 0.193 1.000000e+00
## GSTO2 1.530008e-01 -1.136478 0.250 0.404 1.000000e+00
## CDR2L 1.544435e-01 1.919444 0.292 0.174 1.000000e+00
## ZNF335 1.548385e-01 -1.412075 0.208 0.349 1.000000e+00
## MATN1 1.550099e-01 1.253168 0.208 0.110 1.000000e+00
## ENSGALG00000033089 1.560634e-01 1.089356 0.375 0.275 1.000000e+00
## ASTN1 1.560634e-01 1.011795 0.375 0.275 1.000000e+00
## ARFIP1 1.562135e-01 1.132171 0.750 0.670 1.000000e+00
## HDX 1.567387e-01 1.650612 0.250 0.156 1.000000e+00
## FBXO39 1.567387e-01 1.590399 0.250 0.156 1.000000e+00
## ENSGALG00000000584 1.579434e-01 1.846901 0.208 0.119 1.000000e+00
## ENSGALG00000014960 1.579434e-01 1.199852 0.208 0.119 1.000000e+00
## C1QTNF8 1.579586e-01 -2.729943 0.042 0.147 1.000000e+00
## ENSGALG00000020488 1.581956e-01 -1.448963 0.292 0.468 1.000000e+00
## ENSGALG00000020921 1.587721e-01 -1.058082 0.208 0.367 1.000000e+00
## FGFR3 1.590740e-01 1.098742 0.333 0.229 1.000000e+00
## ENSGALG00000047891 1.598710e-01 1.692180 0.542 0.560 1.000000e+00
## MROH1 1.608241e-01 -1.367218 0.292 0.422 1.000000e+00
## ITGB3 1.608245e-01 2.911469 0.167 0.083 1.000000e+00
## ENSGALG00000052954 1.613453e-01 1.509741 0.292 0.156 1.000000e+00
## PTPN9 1.613453e-01 -1.638210 0.083 0.202 1.000000e+00
## CDC14B 1.616100e-01 1.507988 0.292 0.193 1.000000e+00
## MBOAT2 1.624463e-01 1.633210 0.417 0.358 1.000000e+00
## GDF8 1.625412e-01 1.148946 0.250 0.138 1.000000e+00
## SCO1 1.628988e-01 -1.374130 0.250 0.404 1.000000e+00
## RNF19A 1.630945e-01 1.936406 0.333 0.257 1.000000e+00
## WASHC5 1.630945e-01 1.019263 0.375 0.248 1.000000e+00
## AMBRA1 1.632683e-01 1.661191 0.625 0.523 1.000000e+00
## HSDL2 1.634227e-01 -1.768055 0.167 0.284 1.000000e+00
## ENSGALG00000055093 1.642408e-01 1.785264 0.167 0.083 1.000000e+00
## PPM1M 1.646838e-01 -2.125042 0.083 0.193 1.000000e+00
## RHPN1 1.649351e-01 2.375599 0.458 0.358 1.000000e+00
## SLC7A5 1.653362e-01 -1.879593 0.333 0.486 1.000000e+00
## LRP8 1.655560e-01 1.328174 0.792 0.670 1.000000e+00
## ENSGALG00000039573 1.660198e-01 1.609568 0.375 0.257 1.000000e+00
## YDJC 1.670471e-01 1.575298 0.292 0.174 1.000000e+00
## SH3RF1 1.673984e-01 1.570605 0.250 0.156 1.000000e+00
## PTPN13 1.676620e-01 2.389260 0.500 0.486 1.000000e+00
## CNGA3 1.677115e-01 2.914776 0.167 0.083 1.000000e+00
## MSH3 1.679916e-01 2.030465 0.458 0.394 1.000000e+00
## MYCBPAP 1.688077e-01 1.170132 0.375 0.266 1.000000e+00
## ENSGALG00000048121 1.698572e-01 1.628057 0.208 0.119 1.000000e+00
## ALG8 1.701791e-01 1.240389 0.208 0.110 1.000000e+00
## VTG2 1.710841e-01 -2.382071 0.125 0.239 1.000000e+00
## OTUD4 1.712370e-01 2.194264 0.167 0.083 1.000000e+00
## SLC2A5 1.712370e-01 1.846726 0.167 0.083 1.000000e+00
## FAM174C 1.718897e-01 -1.018728 0.125 0.257 1.000000e+00
## ZNF839 1.719024e-01 1.458533 0.375 0.303 1.000000e+00
## ENSGALG00000007195 1.728346e-01 -1.059599 0.125 0.248 1.000000e+00
## GORASP1 1.728346e-01 -1.598822 0.125 0.248 1.000000e+00
## ENSGALG00000009538 1.731321e-01 1.057966 0.333 0.211 1.000000e+00
## ERCC4 1.732861e-01 -1.978825 0.042 0.138 1.000000e+00
## TCEANC2 1.733421e-01 1.063225 0.208 0.110 1.000000e+00
## GLRX 1.733421e-01 -1.079468 0.042 0.147 1.000000e+00
## ARAP2 1.740229e-01 1.107398 0.583 0.560 1.000000e+00
## FADS2 1.746711e-01 -1.824882 0.167 0.294 1.000000e+00
## TRMT9B 1.748177e-01 1.410860 0.167 0.083 1.000000e+00
## PTPDC1 1.748177e-01 1.029968 0.167 0.083 1.000000e+00
## RBM45 1.757367e-01 1.160634 0.333 0.220 1.000000e+00
## AGA 1.757440e-01 -2.384787 0.083 0.193 1.000000e+00
## EPHA2 1.765489e-01 -1.090706 0.042 0.147 1.000000e+00
## ENSGALG00000051325 1.765489e-01 -1.143579 0.042 0.147 1.000000e+00
## SCAI 1.770583e-01 1.332799 0.375 0.248 1.000000e+00
## ENSGALG00000049055 1.774114e-01 1.188489 0.917 0.945 1.000000e+00
## COLGALT2 1.783267e-01 -1.419732 0.250 0.376 1.000000e+00
## JAM3 1.784006e-01 2.329777 0.375 0.275 1.000000e+00
## ENSGALG00000053842 1.784540e-01 2.829200 0.167 0.083 1.000000e+00
## HECA 1.790326e-01 1.867548 0.125 0.055 1.000000e+00
## ENSGALG00000026015 1.790326e-01 1.771382 0.125 0.055 1.000000e+00
## ENSGALG00000048854 1.790326e-01 1.687334 0.125 0.055 1.000000e+00
## CDS1 1.794448e-01 -1.207236 0.125 0.257 1.000000e+00
## IQUB 1.795271e-01 2.520864 0.292 0.193 1.000000e+00
## ENSGALG00000005350 1.805076e-01 -1.185079 0.833 0.853 1.000000e+00
## TLL1 1.810750e-01 1.298659 0.750 0.734 1.000000e+00
## MARCHF11 1.814777e-01 1.097248 0.250 0.156 1.000000e+00
## FOXP1 1.820034e-01 1.056971 0.417 0.303 1.000000e+00
## ENSGALG00000051044 1.833026e-01 -1.366403 0.042 0.138 1.000000e+00
## C8H1orf52 1.833026e-01 -1.584506 0.042 0.138 1.000000e+00
## ENSGALG00000015203 1.833026e-01 -1.618684 0.042 0.138 1.000000e+00
## SLC15A2 1.833026e-01 -1.889993 0.042 0.138 1.000000e+00
## ABCD2 1.833197e-01 -1.096765 0.208 0.394 1.000000e+00
## IFT52 1.843656e-01 -1.476637 0.167 0.294 1.000000e+00
## WRN 1.847320e-01 1.126071 0.542 0.477 1.000000e+00
## MARC1 1.851212e-01 -1.691969 0.500 0.523 1.000000e+00
## JADE3 1.856813e-01 2.225720 0.208 0.119 1.000000e+00
## ENSGALG00000013765 1.857765e-01 -1.850195 0.125 0.248 1.000000e+00
## CRTAP 1.858949e-01 1.131051 0.167 0.083 1.000000e+00
## CALD1 1.861524e-01 1.386067 0.542 0.514 1.000000e+00
## ENSGALG00000008518 1.861598e-01 -1.731728 0.083 0.202 1.000000e+00
## MBP 1.861998e-01 -1.190013 0.708 0.780 1.000000e+00
## ENSGALG00000037242 1.867351e-01 -1.900211 0.042 0.138 1.000000e+00
## DR1 1.867901e-01 -2.020233 0.125 0.239 1.000000e+00
## ENSGALG00000046774 1.878839e-01 1.698516 0.125 0.055 1.000000e+00
## ADPRHL2 1.880265e-01 -1.286376 0.208 0.339 1.000000e+00
## ALS2 1.890334e-01 -1.272505 0.208 0.376 1.000000e+00
## WDR26 1.893614e-01 1.484854 0.667 0.679 1.000000e+00
## ERMARD 1.897002e-01 1.507874 0.167 0.083 1.000000e+00
## MTMR12 1.897002e-01 1.186184 0.167 0.083 1.000000e+00
## SATB2 1.898394e-01 1.417743 0.792 0.835 1.000000e+00
## CNP 1.898453e-01 1.018972 0.375 0.257 1.000000e+00
## ENSGALG00000048213 1.900544e-01 -1.035371 0.292 0.459 1.000000e+00
## SETDB1 1.902147e-01 -1.371537 0.042 0.138 1.000000e+00
## GGA1 1.902147e-01 -1.837071 0.042 0.138 1.000000e+00
## ENSGALG00000000293 1.924288e-01 1.579479 0.125 0.055 1.000000e+00
## MMP1 1.924288e-01 1.417669 0.125 0.055 1.000000e+00
## ENSGALG00000032841 1.924288e-01 1.410085 0.125 0.055 1.000000e+00
## INTS10 1.926922e-01 1.234601 0.375 0.266 1.000000e+00
## NFKBIE 1.937419e-01 -1.492319 0.042 0.138 1.000000e+00
## PTGR2 1.942884e-01 -1.478631 0.458 0.523 1.000000e+00
## ARHGEF10L 1.948951e-01 1.219770 0.667 0.670 1.000000e+00
## RNPEP 1.948987e-01 1.374778 0.375 0.257 1.000000e+00
## ENSGALG00000048311 1.952908e-01 2.062285 0.292 0.220 1.000000e+00
## PPIL4 1.966354e-01 -1.084185 0.333 0.202 1.000000e+00
## MAPK8IP1 1.966354e-01 -2.041583 0.125 0.248 1.000000e+00
## STRADB 1.967229e-01 1.132861 0.208 0.110 1.000000e+00
## CUEDC1 1.973169e-01 -1.607286 0.042 0.138 1.000000e+00
## SERINC4 1.973169e-01 -1.871504 0.042 0.138 1.000000e+00
## TTC13 1.978369e-01 1.276824 0.292 0.202 1.000000e+00
## SMG7 1.988599e-01 -1.257362 0.458 0.532 1.000000e+00
## SMARCC2 1.988805e-01 1.416253 0.292 0.193 1.000000e+00
## MFSD13A 1.995104e-01 -1.301326 0.083 0.193 1.000000e+00
## ENSGALG00000053916 1.998435e-01 -1.203914 0.333 0.459 1.000000e+00
## TGFBR1 2.003204e-01 1.192790 0.375 0.266 1.000000e+00
## ENSGALG00000054483 2.007355e-01 1.022471 0.250 0.147 1.000000e+00
## USP54 2.007841e-01 1.468613 0.542 0.505 1.000000e+00
## XAF1 2.014602e-01 1.251625 0.167 0.083 1.000000e+00
## ENSGALG00000050633 2.017602e-01 1.118174 0.125 0.055 1.000000e+00
## PLPP4 2.019701e-01 1.086621 0.333 0.220 1.000000e+00
## FAM120B 2.021185e-01 1.405239 0.542 0.413 1.000000e+00
## EARS2 2.025698e-01 1.474750 0.208 0.119 1.000000e+00
## ENSGALG00000053663 2.035363e-01 1.066105 0.292 0.202 1.000000e+00
## TRIM3 2.046112e-01 -1.968459 0.042 0.138 1.000000e+00
## PLEKHH2 2.054581e-01 1.873923 0.250 0.174 1.000000e+00
## ENSGALG00000048445 2.054960e-01 -1.290800 0.167 0.083 1.000000e+00
## PTPN5 2.059061e-01 1.000703 0.583 0.532 1.000000e+00
## LYPLA2 2.071488e-01 -1.263907 0.458 0.514 1.000000e+00
## CTNND1 2.075429e-01 1.222280 0.333 0.220 1.000000e+00
## IQCB1 2.087248e-01 -2.670602 0.042 0.128 1.000000e+00
## CRAT 2.095037e-01 -1.145190 0.250 0.349 1.000000e+00
## CNPPD1 2.095902e-01 1.114509 0.167 0.083 1.000000e+00
## PIGN 2.099693e-01 1.296036 0.458 0.394 1.000000e+00
## FAM89A 2.110140e-01 -1.087826 0.333 0.468 1.000000e+00
## NR4A3 2.114172e-01 1.069588 0.125 0.055 1.000000e+00
## SLC29A3 2.126079e-01 -1.221171 0.042 0.128 1.000000e+00
## GPATCH2L 2.130695e-01 2.535951 0.333 0.257 1.000000e+00
## BEND4 2.130695e-01 1.594778 0.333 0.257 1.000000e+00
## ABLIM3 2.136877e-01 1.158621 0.250 0.165 1.000000e+00
## ENSGALG00000013079 2.136877e-01 -1.358394 0.083 0.202 1.000000e+00
## ENSGALG00000027778 2.148434e-01 1.303197 0.208 0.128 1.000000e+00
## TEX2 2.148545e-01 -1.443022 0.167 0.275 1.000000e+00
## NUP210 2.155767e-01 1.604058 0.375 0.303 1.000000e+00
## WTIP 2.161097e-01 2.983886 0.458 0.385 1.000000e+00
## RNF208 2.162334e-01 -1.041275 0.208 0.303 1.000000e+00
## EYA4 2.164538e-01 -1.242956 0.417 0.303 1.000000e+00
## TSPAN15 2.165594e-01 1.767380 0.167 0.092 1.000000e+00
## GRK7 2.165594e-01 1.567838 0.167 0.092 1.000000e+00
## IGFBP4 2.165594e-01 1.087097 0.167 0.092 1.000000e+00
## ENSGALG00000014719 2.166474e-01 -1.459779 0.125 0.229 1.000000e+00
## CCDC50 2.168213e-01 -1.586460 0.167 0.284 1.000000e+00
## ENSGALG00000051644 2.168660e-01 1.072529 0.208 0.119 1.000000e+00
## GFER 2.183057e-01 -1.396935 0.125 0.239 1.000000e+00
## BCAT1 2.188182e-01 -1.496883 0.083 0.193 1.000000e+00
## ENSGALG00000055108 2.188194e-01 1.181957 0.375 0.284 1.000000e+00
## TMEM186 2.189551e-01 -1.165766 0.208 0.312 1.000000e+00
## ENSGALG00000036085 2.190923e-01 1.061819 0.625 0.495 1.000000e+00
## MAML2 2.199942e-01 1.374234 0.667 0.578 1.000000e+00
## ENSGALG00000054372 2.206715e-01 2.219843 0.167 0.092 1.000000e+00
## ENSGALG00000047004 2.206715e-01 1.502511 0.167 0.092 1.000000e+00
## FGD6 2.214303e-01 2.500694 0.333 0.257 1.000000e+00
## ST14 2.221625e-01 1.021506 0.250 0.156 1.000000e+00
## MIEF2 2.224402e-01 -2.996190 0.167 0.248 1.000000e+00
## INTS8 2.232251e-01 1.001369 0.458 0.404 1.000000e+00
## ENSGALG00000031876 2.237007e-01 1.270061 0.042 0.138 1.000000e+00
## IFIH1 2.239224e-01 -1.211177 0.083 0.183 1.000000e+00
## LEF1 2.248393e-01 -1.433727 0.167 0.092 1.000000e+00
## ENSGALG00000052012 2.263573e-01 1.393965 0.333 0.239 1.000000e+00
## ENSGALG00000054247 2.265224e-01 -2.262711 0.125 0.055 1.000000e+00
## ENSGALG00000047213 2.274517e-01 1.322673 0.417 0.385 1.000000e+00
## XPNPEP3 2.275289e-01 -1.981506 0.125 0.239 1.000000e+00
## APBB1IP 2.279245e-01 1.598375 0.333 0.220 1.000000e+00
## ARSJ 2.312066e-01 -2.148885 0.167 0.284 1.000000e+00
## DCP2 2.319868e-01 1.980148 0.292 0.211 1.000000e+00
## SHF 2.324144e-01 1.116188 0.250 0.156 1.000000e+00
## TMEM233 2.324144e-01 -1.794967 0.083 0.193 1.000000e+00
## NUF2 2.328066e-01 -1.610320 0.042 0.128 1.000000e+00
## ENSGALG00000046212 2.333429e-01 1.280038 0.167 0.092 1.000000e+00
## GTF3C3 2.339542e-01 1.173069 0.375 0.312 1.000000e+00
## PLCE1 2.341998e-01 1.336491 0.500 0.450 1.000000e+00
## ENSGALG00000027132 2.359052e-01 1.796856 0.083 0.193 1.000000e+00
## HELQ 2.367332e-01 -1.327594 0.208 0.349 1.000000e+00
## ENSGALG00000008297 2.380019e-01 -1.672293 0.083 0.183 1.000000e+00
## FILIP1 2.383232e-01 -1.441789 0.208 0.321 1.000000e+00
## GPR149 2.389118e-01 -2.589621 0.208 0.294 1.000000e+00
## EPAS1 2.403972e-01 -1.049789 0.667 0.798 1.000000e+00
## ENSGALG00000050110 2.409452e-01 -1.273178 0.250 0.339 1.000000e+00
## SLC38A4 2.412557e-01 -1.461017 0.042 0.128 1.000000e+00
## ENSGALG00000053949 2.414897e-01 1.395008 0.292 0.211 1.000000e+00
## MIF4GD 2.435669e-01 -1.054299 0.250 0.138 1.000000e+00
## UAP1 2.435669e-01 -1.512547 0.083 0.174 1.000000e+00
## GALNT18 2.442360e-01 1.532969 0.667 0.771 1.000000e+00
## LRFN2 2.446528e-01 1.433689 0.208 0.128 1.000000e+00
## RIC8B 2.447709e-01 1.592042 0.250 0.174 1.000000e+00
## RMI2 2.452714e-01 -1.377808 0.083 0.183 1.000000e+00
## RCC1L 2.465218e-01 -2.206995 0.042 0.119 1.000000e+00
## MIER1 2.468008e-01 1.753208 0.292 0.202 1.000000e+00
## ENSGALG00000000470 2.470050e-01 -1.167089 0.500 0.624 1.000000e+00
## RAB43 2.471213e-01 -1.987314 0.125 0.220 1.000000e+00
## ENSGALG00000048184 2.479752e-01 -1.751247 0.125 0.248 1.000000e+00
## PIK3R5 2.489640e-01 -1.187415 0.083 0.183 1.000000e+00
## MEGF11 2.512414e-01 1.035096 0.708 0.642 1.000000e+00
## ENSGALG00000048239 2.515585e-01 -1.124758 0.292 0.394 1.000000e+00
## POLRMT 2.521906e-01 1.250200 0.292 0.220 1.000000e+00
## NCALD 2.535144e-01 1.003581 0.542 0.523 1.000000e+00
## MAP4K5 2.537867e-01 1.345150 0.375 0.266 1.000000e+00
## CARF 2.553744e-01 2.011810 0.250 0.174 1.000000e+00
## EIF4EBP2 2.555925e-01 -1.809835 0.042 0.119 1.000000e+00
## FLNB 2.556474e-01 1.243576 0.500 0.440 1.000000e+00
## CUTC 2.559232e-01 1.102224 0.417 0.312 1.000000e+00
## DOK5 2.565471e-01 1.153655 0.208 0.128 1.000000e+00
## RNF169 2.568990e-01 1.801879 0.208 0.138 1.000000e+00
## GTPBP10 2.571842e-01 1.257456 0.417 0.358 1.000000e+00
## B2M 2.574702e-01 -1.493995 0.125 0.220 1.000000e+00
## TBC1D2B 2.579304e-01 1.124912 0.292 0.211 1.000000e+00
## HID1 2.590231e-01 1.475641 0.375 0.284 1.000000e+00
## UNK 2.600356e-01 1.039628 0.417 0.358 1.000000e+00
## ENSGALG00000051937 2.602353e-01 1.386159 0.333 0.239 1.000000e+00
## ENSGALG00000044388 2.603716e-01 2.013959 0.167 0.294 1.000000e+00
## AP4B1 2.608678e-01 1.356921 0.208 0.138 1.000000e+00
## TMEM248 2.621509e-01 1.010994 0.250 0.165 1.000000e+00
## IQGAP1 2.624811e-01 1.327465 0.625 0.587 1.000000e+00
## C9orf85 2.624850e-01 1.015375 0.458 0.431 1.000000e+00
## NDST4 2.628022e-01 1.080773 0.583 0.587 1.000000e+00
## LMO2 2.645344e-01 -1.219528 0.125 0.220 1.000000e+00
## ENSGALG00000021092 2.648925e-01 -1.452655 0.042 0.119 1.000000e+00
## ENSGALG00000054390 2.649569e-01 1.100218 0.333 0.266 1.000000e+00
## APRT 2.651596e-01 -1.080010 0.208 0.321 1.000000e+00
## AHCYL2 2.663702e-01 1.283058 0.583 0.596 1.000000e+00
## TUBB1 2.671882e-01 -1.502361 0.125 0.239 1.000000e+00
## HS6ST2 2.672984e-01 1.102820 0.500 0.431 1.000000e+00
## ENSGALG00000052336 2.681161e-01 -1.112297 0.125 0.220 1.000000e+00
## ENSGALG00000052952 2.683199e-01 2.640626 0.125 0.064 1.000000e+00
## ENSGALG00000033919 2.692804e-01 1.440464 0.583 0.514 1.000000e+00
## FUBP3 2.695453e-01 1.375182 0.625 0.651 1.000000e+00
## N-RAS 2.696289e-01 -1.791262 0.042 0.119 1.000000e+00
## ENSGALG00000005896 2.697488e-01 -1.228122 0.208 0.294 1.000000e+00
## PPAT 2.697488e-01 -1.908880 0.208 0.294 1.000000e+00
## ENSGALG00000051328 2.738923e-01 1.884424 0.125 0.064 1.000000e+00
## EDEM3 2.738923e-01 1.441682 0.125 0.064 1.000000e+00
## ENSGALG00000047664 2.738923e-01 1.000573 0.125 0.064 1.000000e+00
## BBIP1 2.742928e-01 -1.070846 0.417 0.514 1.000000e+00
## ST7L 2.743187e-01 1.031491 0.625 0.541 1.000000e+00
## ENSGALG00000048149 2.744230e-01 -1.452259 0.042 0.119 1.000000e+00
## ENSGALG00000035116 2.749935e-01 1.409865 0.375 0.312 1.000000e+00
## ENSGALG00000017706 2.756649e-01 1.147698 0.875 0.844 1.000000e+00
## TTC8 2.765590e-01 -1.621393 0.375 0.505 1.000000e+00
## CD276 2.774940e-01 1.460663 0.250 0.183 1.000000e+00
## ENSGALG00000049391 2.791028e-01 2.597924 0.458 0.349 1.000000e+00
## C1GALT1C1 2.792751e-01 -1.116266 0.042 0.119 1.000000e+00
## ENSGALG00000053965 2.792751e-01 -1.188994 0.042 0.119 1.000000e+00
## SAP30 2.795433e-01 1.080139 0.125 0.064 1.000000e+00
## HTR4 2.804377e-01 -1.085265 0.417 0.486 1.000000e+00
## PHF11 2.813468e-01 1.088296 0.208 0.138 1.000000e+00
## UBE2J2 2.827753e-01 -1.591449 0.125 0.220 1.000000e+00
## TK1 2.832469e-01 -1.324396 0.167 0.257 1.000000e+00
## SH3RF3 2.837445e-01 1.018448 0.417 0.339 1.000000e+00
## HDC 2.843276e-01 2.228683 0.167 0.101 1.000000e+00
## ENSGALG00000047797 2.852730e-01 1.117640 0.125 0.064 1.000000e+00
## ENSGALG00000054607 2.852730e-01 1.018775 0.125 0.064 1.000000e+00
## TRHDE 2.861065e-01 1.153988 0.500 0.780 1.000000e+00
## ENSGALG00000053239 2.865234e-01 -1.098817 0.125 0.220 1.000000e+00
## ZC3H7B 2.867700e-01 -1.299871 0.417 0.330 1.000000e+00
## ENSGALG00000053184 2.871924e-01 1.322521 0.500 0.459 1.000000e+00
## DVL3 2.877499e-01 1.012862 0.625 0.615 1.000000e+00
## SPATA2 2.898361e-01 1.082567 0.208 0.138 1.000000e+00
## ENSGALG00000002470 2.898361e-01 -1.502602 0.083 0.165 1.000000e+00
## FITM2 2.898361e-01 -1.629716 0.083 0.165 1.000000e+00
## ENSGALG00000050189 2.909746e-01 -1.584880 0.042 0.110 1.000000e+00
## WDR89 2.909746e-01 -2.222373 0.042 0.110 1.000000e+00
## ENSGALG00000053451 2.910816e-01 1.498887 0.125 0.064 1.000000e+00
## PAQR8 2.910816e-01 1.008253 0.125 0.064 1.000000e+00
## PDLIM4 2.918817e-01 2.085993 0.417 0.385 1.000000e+00
## ABCB7 2.925841e-01 -1.029367 0.542 0.440 1.000000e+00
## MSRB3 2.938819e-01 1.368093 0.458 0.394 1.000000e+00
## CPA6 2.941447e-01 -1.318274 0.208 0.138 1.000000e+00
## ENSGALG00000044479 2.941447e-01 -1.893674 0.083 0.165 1.000000e+00
## HMGN1 2.952081e-01 -1.804000 0.250 0.330 1.000000e+00
## POLR1B 2.955574e-01 -1.027077 0.333 0.440 1.000000e+00
## ZMAT3 2.966193e-01 -1.111483 0.125 0.211 1.000000e+00
## GPR180 2.968343e-01 -2.533804 0.083 0.156 1.000000e+00
## C7orf25 2.968343e-01 -2.925569 0.083 0.156 1.000000e+00
## DUSP23 2.974868e-01 -1.494762 0.167 0.257 1.000000e+00
## SLC15A4 2.979683e-01 -1.816806 0.125 0.220 1.000000e+00
## SLC37A3 2.984961e-01 1.353297 0.208 0.138 1.000000e+00
## PUS1 2.989495e-01 1.143598 0.167 0.101 1.000000e+00
## MTFR1 2.993545e-01 -1.332116 0.167 0.266 1.000000e+00
## ENSGALG00000034065 3.005186e-01 1.005708 0.083 0.183 1.000000e+00
## FHL5 3.005477e-01 2.378558 0.250 0.183 1.000000e+00
## CRCP 3.009069e-01 -1.027028 0.500 0.560 1.000000e+00
## ATAD2 3.011084e-01 1.323406 0.250 0.165 1.000000e+00
## INSIG2 3.011114e-01 -1.035583 0.333 0.404 1.000000e+00
## TMBIM1 3.014745e-01 -1.046958 0.042 0.110 1.000000e+00
## KIF26A 3.024563e-01 1.050116 0.667 0.615 1.000000e+00
## ENSGALG00000048173 3.024789e-01 1.216605 0.417 0.349 1.000000e+00
## ENSGALG00000034586 3.039331e-01 2.358283 0.167 0.101 1.000000e+00
## ENSGALG00000052782 3.042307e-01 -1.866122 0.375 0.394 1.000000e+00
## CHMP1A 3.065980e-01 -1.068642 0.167 0.266 1.000000e+00
## VEZF1 3.073272e-01 -1.056013 0.083 0.165 1.000000e+00
## ENSGALG00000048039 3.085048e-01 -1.347936 0.208 0.312 1.000000e+00
## ENSGALG00000001533 3.089716e-01 2.299432 0.167 0.101 1.000000e+00
## ODF2 3.093372e-01 1.454555 0.542 0.596 1.000000e+00
## GUF1 3.102630e-01 -1.094169 0.333 0.229 1.000000e+00
## BDH2 3.103917e-01 -1.812450 0.083 0.156 1.000000e+00
## CCDC103 3.118389e-01 -1.825141 0.167 0.239 1.000000e+00
## ENC1 3.122236e-01 -1.320696 0.042 0.110 1.000000e+00
## ENSGALG00000012808 3.144052e-01 1.217547 0.833 0.826 1.000000e+00
## UBXN10 3.148683e-01 -1.006206 0.167 0.092 1.000000e+00
## THUMPD2 3.156795e-01 1.639333 0.292 0.211 1.000000e+00
## C5H11ORF96 3.156795e-01 -1.165130 0.167 0.239 1.000000e+00
## KIAA1524 3.163296e-01 -2.657029 0.083 0.165 1.000000e+00
## CCM2 3.176917e-01 -1.057438 0.042 0.110 1.000000e+00
## GPR1 3.176917e-01 -1.247008 0.042 0.110 1.000000e+00
## ENSGALG00000054067 3.176917e-01 -1.555716 0.042 0.110 1.000000e+00
## PGGT1B 3.177199e-01 1.063948 0.250 0.165 1.000000e+00
## CYP1B1 3.192133e-01 1.187534 0.167 0.101 1.000000e+00
## ENSGALG00000052793 3.192133e-01 -1.233073 0.167 0.101 1.000000e+00
## ENSGALG00000007833 3.207080e-01 -1.361471 0.125 0.211 1.000000e+00
## MDM1 3.208951e-01 -2.018866 0.083 0.165 1.000000e+00
## CTU2 3.213112e-01 -1.363429 0.125 0.064 1.000000e+00
## FOXJ1 3.213112e-01 -1.568156 0.125 0.064 1.000000e+00
## SLC39A3 3.217342e-01 1.306779 0.333 0.257 1.000000e+00
## HIPK1 3.219649e-01 -1.388318 0.250 0.165 1.000000e+00
## FAM83B 3.219706e-01 -2.237720 0.333 0.422 1.000000e+00
## TMCC2 3.224115e-01 -1.072093 0.250 0.339 1.000000e+00
## TMEM67 3.227449e-01 1.026794 0.333 0.266 1.000000e+00
## NEPRO 3.232223e-01 -1.443676 0.042 0.110 1.000000e+00
## LSP1P1 3.233173e-01 1.516988 0.292 0.239 1.000000e+00
## PAXIP1 3.233173e-01 1.170271 0.292 0.239 1.000000e+00
## PMS2 3.235591e-01 -1.494796 0.125 0.202 1.000000e+00
## ENSGALG00000031929 3.248437e-01 -1.359927 0.125 0.211 1.000000e+00
## ENSGALG00000050951 3.249925e-01 1.538721 0.417 0.358 1.000000e+00
## ENSGALG00000044620 3.256595e-01 1.169135 0.375 0.303 1.000000e+00
## ENSGALG00000050978 3.279002e-01 2.131108 0.250 0.193 1.000000e+00
## CYP2D6 3.288152e-01 -1.344169 0.042 0.110 1.000000e+00
## ENSGALG00000054100 3.298531e-01 1.611465 0.667 0.670 1.000000e+00
## TSTD2 3.301545e-01 1.527223 0.208 0.138 1.000000e+00
## CPSF2 3.313344e-01 1.015895 0.542 0.569 1.000000e+00
## MBTPS1 3.313920e-01 1.194154 0.292 0.220 1.000000e+00
## ENSGALG00000006851 3.321079e-01 1.245070 0.417 0.349 1.000000e+00
## ZNF770 3.324315e-01 -1.263495 0.208 0.119 1.000000e+00
## GLTP 3.332188e-01 -1.105167 0.125 0.211 1.000000e+00
## ENSGALG00000023661 3.338817e-01 -1.531480 0.083 0.156 1.000000e+00
## ENSGALG00000041782 3.353464e-01 1.001310 0.292 0.211 1.000000e+00
## ENSGALG00000042733 3.373227e-01 -1.295152 0.167 0.248 1.000000e+00
## ENSGALG00000033143 3.373633e-01 -1.455503 0.083 0.147 1.000000e+00
## LRRC47 3.377072e-01 1.090552 0.208 0.147 1.000000e+00
## ENSGALG00000032534 3.380895e-01 1.222183 0.583 0.578 1.000000e+00
## ENSGALG00000004637 3.393725e-01 1.125640 0.292 0.211 1.000000e+00
## SNTA1 3.395851e-01 1.025761 0.208 0.138 1.000000e+00
## ENSGALG00000047652 3.407278e-01 2.096476 0.500 0.468 1.000000e+00
## CAPN2 3.408388e-01 1.399042 0.458 0.385 1.000000e+00
## ALDH6A1 3.413142e-01 -2.371054 0.167 0.248 1.000000e+00
## UTP20 3.413233e-01 1.517802 0.417 0.367 1.000000e+00
## ENSGALG00000049104 3.417320e-01 -1.695707 0.125 0.211 1.000000e+00
## HTR2C 3.420890e-01 1.810752 0.583 0.560 1.000000e+00
## SH2D3C 3.430022e-01 -1.085932 0.333 0.440 1.000000e+00
## CHAMP1 3.432379e-01 -1.633125 0.250 0.321 1.000000e+00
## ENSGALG00000051678 3.432849e-01 -1.135971 0.042 0.101 1.000000e+00
## ZBTB20 3.432849e-01 -1.154412 0.042 0.101 1.000000e+00
## DPH2 3.433795e-01 1.427554 0.167 0.110 1.000000e+00
## LFNG 3.435907e-01 -1.668049 0.083 0.156 1.000000e+00
## ENSGALG00000027620 3.445416e-01 -1.267278 0.292 0.367 1.000000e+00
## USE1 3.445439e-01 -1.094039 0.167 0.266 1.000000e+00
## MTR 3.446351e-01 1.431736 0.250 0.193 1.000000e+00
## DCP1B 3.456074e-01 1.611496 0.125 0.073 1.000000e+00
## ENSGALG00000054283 3.456074e-01 1.266152 0.125 0.073 1.000000e+00
## ENSGALG00000037052 3.456074e-01 1.057024 0.125 0.073 1.000000e+00
## MYO1E 3.456986e-01 -1.083197 0.292 0.440 1.000000e+00
## ENSGALG00000041442 3.473672e-01 -2.348723 0.083 0.147 1.000000e+00
## SPRED2 3.484218e-01 1.099905 0.458 0.459 1.000000e+00
## HSD11B1L 3.493133e-01 -1.009560 0.042 0.101 1.000000e+00
## ENSGALG00000037112 3.493133e-01 -1.117900 0.042 0.101 1.000000e+00
## BORA 3.493133e-01 -1.281174 0.042 0.101 1.000000e+00
## ENSGALG00000055057 3.493133e-01 -1.293444 0.042 0.101 1.000000e+00
## ENSGALG00000041514 3.493133e-01 -2.130180 0.042 0.101 1.000000e+00
## SYT10 3.494485e-01 1.169060 0.083 0.174 1.000000e+00
## ABCG1 3.499362e-01 1.600631 0.333 0.284 1.000000e+00
## PER3 3.501830e-01 2.098114 0.375 0.339 1.000000e+00
## KCNH7 3.504839e-01 1.154148 0.292 0.239 1.000000e+00
## LONRF1 3.518275e-01 1.562983 0.208 0.147 1.000000e+00
## ENSGALG00000047787 3.519078e-01 1.935029 0.125 0.073 1.000000e+00
## TWISTNB 3.519078e-01 1.346665 0.125 0.073 1.000000e+00
## ENSGALG00000006766 3.531438e-01 1.585332 0.833 0.872 1.000000e+00
## MBD3 3.532147e-01 -1.331613 0.208 0.303 1.000000e+00
## MTX3 3.538840e-01 -1.974960 0.125 0.202 1.000000e+00
## ENSGALG00000047870 3.540516e-01 1.150754 0.208 0.138 1.000000e+00
## ENSGALG00000010854 3.542807e-01 1.876506 0.708 0.798 1.000000e+00
## TSEN2 3.568247e-01 -1.244803 0.625 0.633 1.000000e+00
## ENSGALG00000050384 3.582805e-01 1.278971 0.125 0.073 1.000000e+00
## UNC50 3.587069e-01 -1.104127 0.292 0.367 1.000000e+00
## ACOT9 3.591566e-01 -1.216830 0.167 0.257 1.000000e+00
## ENSGALG00000047816 3.597172e-01 -1.331189 0.125 0.220 1.000000e+00
## LRATD1 3.615704e-01 -1.624858 0.042 0.101 1.000000e+00
## TEKT1 3.619051e-01 1.141129 0.250 0.193 1.000000e+00
## ENSGALG00000050631 3.623744e-01 -1.091937 0.167 0.101 1.000000e+00
## ENSGALG00000001894 3.628680e-01 -2.673135 0.125 0.202 1.000000e+00
## CTSH 3.643422e-01 1.621286 0.250 0.202 1.000000e+00
## ENSGALG00000007030 3.647253e-01 2.117493 0.125 0.073 1.000000e+00
## SLAIN1 3.648894e-01 1.742900 0.167 0.110 1.000000e+00
## SOCS6 3.648894e-01 1.359262 0.167 0.110 1.000000e+00
## NPY2R 3.677988e-01 -1.189023 0.042 0.101 1.000000e+00
## CEP78 3.677988e-01 -1.883648 0.042 0.101 1.000000e+00
## METTL6 3.682851e-01 1.627521 0.208 0.156 1.000000e+00
## RAB9B 3.686428e-01 -1.650277 0.083 0.156 1.000000e+00
## FBXO40 3.686441e-01 1.050772 0.208 0.303 1.000000e+00
## NSUN6 3.686908e-01 -1.570365 0.167 0.266 1.000000e+00
## PCIF1 3.703956e-01 1.881186 0.167 0.110 1.000000e+00
## ABCA5 3.707284e-01 1.351397 0.333 0.248 1.000000e+00
## ENSGALG00000040949 3.712420e-01 1.662303 0.125 0.073 1.000000e+00
## HELB 3.712420e-01 1.237774 0.125 0.073 1.000000e+00
## PTTG1IP 3.737930e-01 -1.150039 0.125 0.064 1.000000e+00
## QSOX2 3.748464e-01 -1.026954 0.208 0.312 1.000000e+00
## TRPV1 3.769929e-01 1.179578 0.292 0.211 1.000000e+00
## ZCCHC14 3.783104e-01 1.803321 0.458 0.440 1.000000e+00
## TARBP1 3.790892e-01 1.619168 0.667 0.587 1.000000e+00
## KCTD6 3.802677e-01 1.314816 0.375 0.321 1.000000e+00
## SNX21 3.811711e-01 1.102063 0.208 0.147 1.000000e+00
## ENSGALG00000028041 3.819630e-01 1.428580 0.125 0.229 1.000000e+00
## SLC5A9 3.829008e-01 1.386449 0.208 0.156 1.000000e+00
## ENSGALG00000015652 3.831091e-01 1.579251 0.375 0.339 1.000000e+00
## PYROXD1 3.838474e-01 -1.309407 0.083 0.147 1.000000e+00
## TMEM214 3.839502e-01 1.381397 0.250 0.211 1.000000e+00
## DCLRE1C 3.844906e-01 2.354411 0.125 0.073 1.000000e+00
## ARHGAP35 3.844906e-01 1.195179 0.125 0.073 1.000000e+00
## USP2 3.847909e-01 -1.780193 0.250 0.321 1.000000e+00
## ENSGALG00000052259 3.863447e-01 -1.506872 0.292 0.330 1.000000e+00
## ZFYVE26 3.864485e-01 1.479663 0.250 0.202 1.000000e+00
## ENSGALG00000050348 3.868816e-01 -1.862556 0.042 0.101 1.000000e+00
## ANAPC7 3.877164e-01 -1.024679 0.375 0.248 1.000000e+00
## CD63 3.881847e-01 1.174493 0.333 0.266 1.000000e+00
## ENSGALG00000055056 3.887025e-01 1.494482 0.333 0.284 1.000000e+00
## ZRANB1 3.887025e-01 -1.062711 0.417 0.266 1.000000e+00
## PTCD1 3.892439e-01 -1.553333 0.083 0.147 1.000000e+00
## SVOPL 3.912220e-01 -1.093770 0.125 0.073 1.000000e+00
## DUSP14 3.915502e-01 -1.221815 0.167 0.248 1.000000e+00
## LDLRAD3 3.921512e-01 1.236887 0.417 0.358 1.000000e+00
## STAG2 3.940679e-01 1.135479 0.583 0.624 1.000000e+00
## CARMIL2 3.945081e-01 -1.154128 0.167 0.239 1.000000e+00
## POLR3H 3.945081e-01 -1.931875 0.167 0.239 1.000000e+00
## ENSGALG00000046360 3.960073e-01 1.401842 0.292 0.239 1.000000e+00
## TRMU 3.973609e-01 -1.212512 0.167 0.229 1.000000e+00
## BACE2 3.981580e-01 -1.561442 0.167 0.266 1.000000e+00
## NCAPH 3.997098e-01 -1.997214 0.083 0.138 1.000000e+00
## TSPAN14 3.997428e-01 1.604456 0.083 0.165 1.000000e+00
## CA3B 3.999328e-01 3.171529 0.042 0.101 1.000000e+00
## ENSGALG00000050514 4.002130e-01 -1.509253 0.125 0.202 1.000000e+00
## ENSGALG00000039134 4.006335e-01 1.030732 0.333 0.303 1.000000e+00
## SEPTIN10 4.015438e-01 1.728150 0.208 0.147 1.000000e+00
## ARHGAP1 4.015892e-01 -1.729314 0.417 0.468 1.000000e+00
## KCNMB4 4.016950e-01 -1.113120 0.417 0.550 1.000000e+00
## COLEC12 4.025984e-01 -1.555642 0.208 0.294 1.000000e+00
## ENSGALG00000047366 4.026762e-01 1.572520 0.458 0.422 1.000000e+00
## RBM48 4.027031e-01 1.126539 0.250 0.193 1.000000e+00
## NXPH1 4.034049e-01 -1.967743 0.417 0.477 1.000000e+00
## ENSGALG00000049093 4.045060e-01 1.584574 0.167 0.110 1.000000e+00
## ENSGALG00000040710 4.050298e-01 1.267619 0.083 0.165 1.000000e+00
## SHISA5 4.057084e-01 -1.150860 0.083 0.147 1.000000e+00
## ENSGALG00000012847 4.057084e-01 -1.446591 0.083 0.147 1.000000e+00
## TNFRSF19 4.090157e-01 1.006960 0.333 0.275 1.000000e+00
## GPSM1 4.090295e-01 1.415500 0.333 0.284 1.000000e+00
## ZNF512 4.099497e-01 -1.492738 0.125 0.211 1.000000e+00
## POMK 4.103090e-01 -1.428383 0.208 0.275 1.000000e+00
## CYP39A1 4.103583e-01 -1.213994 0.208 0.138 1.000000e+00
## HS3ST2 4.110216e-01 1.063306 0.083 0.156 1.000000e+00
## NFYA 4.131795e-01 1.421686 0.208 0.156 1.000000e+00
## ENSGALG00000051186 4.131795e-01 1.268554 0.208 0.156 1.000000e+00
## COL3A1 4.140311e-01 1.094255 0.250 0.202 1.000000e+00
## TXNDC5 4.156747e-01 -1.831630 0.167 0.229 1.000000e+00
## NGB 4.162810e-01 2.565451 0.167 0.110 1.000000e+00
## EXD2 4.162810e-01 1.200648 0.167 0.110 1.000000e+00
## ENSGALG00000054537 4.168587e-01 1.135261 0.167 0.119 1.000000e+00
## ENSGALG00000038612 4.168925e-01 1.743034 0.250 0.193 1.000000e+00
## RMDN1 4.170813e-01 -1.620610 0.167 0.239 1.000000e+00
## LMO4 4.176342e-01 -1.012344 0.375 0.450 1.000000e+00
## RAP2A 4.183603e-01 1.839068 0.208 0.156 1.000000e+00
## FAM91A1 4.183603e-01 1.122723 0.208 0.156 1.000000e+00
## ENSGALG00000048924 4.188552e-01 1.934322 0.125 0.073 1.000000e+00
## PLSCR5 4.197215e-01 -1.284833 0.125 0.202 1.000000e+00
## ITPKA 4.215572e-01 -2.110357 0.375 0.468 1.000000e+00
## LPAR1 4.216871e-01 1.331453 0.250 0.193 1.000000e+00
## ENSGALG00000046887 4.220517e-01 2.217161 0.083 0.156 1.000000e+00
## SLC35F4 4.229563e-01 1.093478 0.792 0.817 1.000000e+00
## TPH2 4.250155e-01 -1.753664 0.167 0.229 1.000000e+00
## TRMT2B 4.285286e-01 1.237110 0.167 0.119 1.000000e+00
## SLC25A26 4.287217e-01 1.195920 0.375 0.330 1.000000e+00
## KCNK9 4.289392e-01 1.629752 0.125 0.220 1.000000e+00
## OSTF1 4.309833e-01 -1.659936 0.167 0.239 1.000000e+00
## ENSGALG00000052541 4.320212e-01 -1.099235 0.167 0.248 1.000000e+00
## GRIP2 4.329307e-01 1.295252 0.333 0.312 1.000000e+00
## ENSGALG00000048159 4.330919e-01 -1.375987 0.125 0.073 1.000000e+00
## MELTF 4.336986e-01 1.970482 0.125 0.083 1.000000e+00
## ARID3B 4.340558e-01 -1.131540 0.083 0.147 1.000000e+00
## FGF10 4.345069e-01 -1.076077 0.208 0.284 1.000000e+00
## EXOSC8 4.346198e-01 1.177827 0.208 0.165 1.000000e+00
## ZNF277 4.346198e-01 -1.588609 0.125 0.183 1.000000e+00
## ENSGALG00000032417 4.365526e-01 -1.865995 0.208 0.275 1.000000e+00
## IBA57 4.373298e-01 -1.124786 0.333 0.385 1.000000e+00
## ENSGALG00000002499 4.394243e-01 1.040924 0.458 0.495 1.000000e+00
## SULF1 4.394628e-01 1.717640 0.208 0.156 1.000000e+00
## DMA 4.398585e-01 -1.071891 0.125 0.183 1.000000e+00
## D2HGDH 4.398585e-01 -1.428156 0.125 0.183 1.000000e+00
## ENSGALG00000054714 4.403863e-01 1.000198 0.167 0.119 1.000000e+00
## MTHFS 4.404289e-01 -1.988668 0.167 0.110 1.000000e+00
## ENSGALG00000053551 4.406443e-01 2.519332 0.125 0.083 1.000000e+00
## EMG1 4.427457e-01 -1.227122 0.167 0.220 1.000000e+00
## ENSGALG00000041405 4.443761e-01 1.522509 0.125 0.211 1.000000e+00
## ENSGALG00000035027 4.448323e-01 1.228105 0.208 0.156 1.000000e+00
## BHLHE23 4.449290e-01 -1.280889 0.208 0.257 1.000000e+00
## GCNT2 4.451379e-01 -1.570764 0.125 0.193 1.000000e+00
## DERA 4.457076e-01 -1.792303 0.083 0.147 1.000000e+00
## ENSGALG00000017139 4.459149e-01 1.506412 0.500 0.450 1.000000e+00
## ENSGALG00000033199 4.461401e-01 1.386138 0.250 0.193 1.000000e+00
## C19orf44 4.463850e-01 -1.053713 0.083 0.138 1.000000e+00
## ENSGALG00000003378 4.476534e-01 1.888471 0.125 0.083 1.000000e+00
## AGTR1 4.476534e-01 1.458713 0.125 0.083 1.000000e+00
## POPDC2 4.476534e-01 1.113533 0.125 0.083 1.000000e+00
## BBS4 4.477784e-01 1.078491 0.542 0.532 1.000000e+00
## KCTD3 4.478124e-01 -1.448367 0.208 0.284 1.000000e+00
## CTNS 4.499310e-01 1.610154 0.250 0.220 1.000000e+00
## IFT46 4.502660e-01 -1.035997 0.292 0.358 1.000000e+00
## ENSGALG00000022644 4.503572e-01 -1.893624 0.125 0.193 1.000000e+00
## ENSGALG00000048648 4.503744e-01 1.002014 0.083 0.156 1.000000e+00
## ENSGALG00000050593 4.504439e-01 2.345444 0.208 0.165 1.000000e+00
## LRRK1 4.519079e-01 1.048846 0.208 0.303 1.000000e+00
## MTRR 4.521540e-01 1.035869 0.292 0.248 1.000000e+00
## ZNF106 4.521540e-01 1.023107 0.292 0.248 1.000000e+00
## MANBAL4 4.547254e-01 1.431640 0.125 0.083 1.000000e+00
## GNAI2 4.551087e-01 -1.167645 0.333 0.422 1.000000e+00
## PRLR 4.553904e-01 1.349450 0.333 0.294 1.000000e+00
## ENSGALG00000038896 4.563327e-01 -2.207292 0.250 0.294 1.000000e+00
## FRMPD4 4.564998e-01 1.362776 0.708 0.734 1.000000e+00
## ENSGALG00000037435 4.585209e-01 2.395904 0.167 0.119 1.000000e+00
## RASEF 4.586588e-01 -1.242576 0.167 0.229 1.000000e+00
## LCA5L 4.590554e-01 -1.791042 0.083 0.128 1.000000e+00
## EPS8 4.608300e-01 -1.014946 0.167 0.257 1.000000e+00
## RBPMS 4.611636e-01 -1.269770 0.125 0.174 1.000000e+00
## LNX1 4.613880e-01 1.285208 0.292 0.248 1.000000e+00
## ENSGALG00000036286 4.618599e-01 1.459571 0.125 0.083 1.000000e+00
## FHL2 4.618599e-01 1.277351 0.125 0.083 1.000000e+00
## ID2 4.635839e-01 -1.012371 0.167 0.229 1.000000e+00
## FASTKD3 4.646576e-01 -1.309884 0.083 0.138 1.000000e+00
## TBC1D22B 4.646576e-01 -1.417708 0.083 0.138 1.000000e+00
## ENSGALG00000047509 4.658954e-01 -2.052096 0.167 0.266 1.000000e+00
## PGF 4.664622e-01 1.066010 0.208 0.147 1.000000e+00
## SLC26A7 4.675209e-01 1.980869 0.250 0.202 1.000000e+00
## SGCB 4.682768e-01 -1.373197 0.542 0.596 1.000000e+00
## CCDC82 4.690564e-01 1.301837 0.125 0.083 1.000000e+00
## ENSGALG00000008326 4.708396e-01 1.672496 0.167 0.119 1.000000e+00
## KAT6A 4.708402e-01 -1.200883 0.625 0.651 1.000000e+00
## ENSGALG00000048728 4.717143e-01 -1.182128 0.167 0.110 1.000000e+00
## IL6ST 4.720417e-01 1.609581 0.208 0.165 1.000000e+00
## CNOT6L 4.730049e-01 2.134499 0.292 0.257 1.000000e+00
## ENSGALG00000047824 4.751059e-01 1.180911 0.292 0.266 1.000000e+00
## ENSGALG00000043257 4.770668e-01 -1.140978 0.083 0.138 1.000000e+00
## ENSGALG00000050133 4.781152e-01 -1.578763 0.083 0.128 1.000000e+00
## ENSGALG00000053675 4.786503e-01 -1.341020 0.375 0.486 1.000000e+00
## PRKAB1 4.814979e-01 1.520451 0.125 0.202 1.000000e+00
## RBM18 4.822915e-01 -1.246622 0.292 0.339 1.000000e+00
## NR6A1 4.823729e-01 -1.001910 0.208 0.275 1.000000e+00
## NKIRAS2 4.826337e-01 -1.010023 0.333 0.358 1.000000e+00
## MED22 4.832183e-01 1.168981 0.375 0.330 1.000000e+00
## ENSGALG00000048080 4.833869e-01 -1.074942 0.333 0.275 1.000000e+00
## GINS1 4.845632e-01 -1.731036 0.083 0.128 1.000000e+00
## ENSGALG00000004504 4.878478e-01 -1.233985 0.125 0.193 1.000000e+00
## PARP4 4.882029e-01 -1.135719 0.542 0.495 1.000000e+00
## ENSGALG00000052543 4.886074e-01 1.874393 0.208 0.165 1.000000e+00
## ENSGALG00000023083 4.910137e-01 1.446975 0.125 0.083 1.000000e+00
## THSD4 4.930605e-01 1.153585 0.625 0.624 1.000000e+00
## GLI3 4.944645e-01 -1.132940 0.292 0.385 1.000000e+00
## PITPNB 4.956101e-01 1.058765 0.583 0.596 1.000000e+00
## VRK2 4.966120e-01 1.172459 0.292 0.257 1.000000e+00
## NTHL1 4.975996e-01 -1.695956 0.083 0.128 1.000000e+00
## DAPK2 4.978260e-01 1.753949 0.417 0.431 1.000000e+00
## ADPRH 4.978260e-01 -1.136755 0.417 0.431 1.000000e+00
## ENSGALG00000051601 5.020277e-01 1.310452 0.292 0.239 1.000000e+00
## UNC5D 5.033689e-01 1.559308 0.708 0.761 1.000000e+00
## ENSGALG00000047907 5.039352e-01 2.368554 0.417 0.385 1.000000e+00
## FBXO8 5.041871e-01 -1.117868 0.167 0.110 1.000000e+00
## GK5 5.043987e-01 1.049076 0.250 0.220 1.000000e+00
## FAT1 5.043987e-01 1.041461 0.250 0.220 1.000000e+00
## ENSGALG00000052168 5.059532e-01 1.200170 0.125 0.083 1.000000e+00
## ENSGALG00000052611 5.065676e-01 1.773916 0.083 0.147 1.000000e+00
## KCNH6 5.077219e-01 1.104012 0.417 0.367 1.000000e+00
## NDST1 5.088685e-01 1.549364 0.167 0.119 1.000000e+00
## ENSGALG00000048686 5.088685e-01 -1.499269 0.083 0.138 1.000000e+00
## ENSGALG00000050961 5.095171e-01 -2.167967 0.167 0.239 1.000000e+00
## ENSGALG00000049424 5.100043e-01 -1.084235 0.250 0.165 1.000000e+00
## ENSGALG00000047686 5.101707e-01 2.235798 0.125 0.092 1.000000e+00
## ENSGALG00000048359 5.101707e-01 1.032709 0.125 0.092 1.000000e+00
## TMEM86A 5.108203e-01 -1.252944 0.083 0.128 1.000000e+00
## BET1 5.108203e-01 -1.837189 0.083 0.128 1.000000e+00
## DUT 5.119074e-01 -2.895460 0.208 0.257 1.000000e+00
## DGAT2 5.127892e-01 -1.033608 0.208 0.147 1.000000e+00
## ENSGALG00000041372 5.128851e-01 2.547414 0.167 0.128 1.000000e+00
## XXYLT1 5.135034e-01 -1.085508 0.333 0.248 1.000000e+00
## LZTR1 5.146962e-01 1.057688 0.417 0.394 1.000000e+00
## MARF1 5.148990e-01 -1.159509 0.708 0.716 1.000000e+00
## ENSGALG00000046412 5.153596e-01 1.273522 0.167 0.119 1.000000e+00
## OPRK1 5.153596e-01 -1.055957 0.083 0.138 1.000000e+00
## SPAG6 5.153596e-01 -1.229394 0.167 0.119 1.000000e+00
## STAM 5.168991e-01 1.000995 0.208 0.165 1.000000e+00
## APLF 5.179484e-01 -1.541199 0.125 0.174 1.000000e+00
## ENSGALG00000010433 5.179484e-01 -2.358669 0.125 0.174 1.000000e+00
## ENSGALG00000048034 5.192991e-01 -1.037931 0.167 0.220 1.000000e+00
## ENSGALG00000050592 5.196602e-01 1.731331 0.250 0.211 1.000000e+00
## LDB2 5.207337e-01 -3.088683 0.292 0.394 1.000000e+00
## CREB5 5.210295e-01 1.541963 0.750 0.716 1.000000e+00
## ENSGALG00000035970 5.218934e-01 1.180009 0.167 0.119 1.000000e+00
## ENO4 5.226576e-01 1.450582 0.208 0.165 1.000000e+00
## ENSGALG00000029796 5.226576e-01 -1.301823 0.125 0.183 1.000000e+00
## ENSGALG00000050916 5.239053e-01 -1.345106 0.208 0.266 1.000000e+00
## MMRN1 5.248660e-01 1.465589 0.125 0.092 1.000000e+00
## ENSGALG00000036263 5.256423e-01 1.146322 0.167 0.128 1.000000e+00
## ENSGALG00000054048 5.270987e-01 1.192650 0.792 0.899 1.000000e+00
## MBTPS2 5.281276e-01 1.153683 0.375 0.339 1.000000e+00
## GRIN2A 5.284046e-01 -1.036419 0.708 0.771 1.000000e+00
## ENSGALG00000054794 5.288880e-01 1.003831 0.292 0.248 1.000000e+00
## ENSGALG00000053997 5.295027e-01 -1.588246 0.500 0.349 1.000000e+00
## TRIM33 5.302192e-01 1.046761 0.250 0.211 1.000000e+00
## FAM219B 5.322941e-01 1.440215 0.125 0.092 1.000000e+00
## ENSGALG00000050146 5.322941e-01 1.054006 0.125 0.092 1.000000e+00
## TMOD3 5.326459e-01 1.492536 0.250 0.367 1.000000e+00
## CMTM4 5.356629e-01 -2.882066 0.125 0.174 1.000000e+00
## LURAP1L 5.365346e-01 -1.442322 0.125 0.083 1.000000e+00
## ENSGALG00000050904 5.371961e-01 1.402032 0.667 0.752 1.000000e+00
## BAALC 5.384227e-01 1.417129 0.208 0.174 1.000000e+00
## ITCH 5.385529e-01 1.090294 0.458 0.486 1.000000e+00
## ENSGALG00000053806 5.397751e-01 1.023768 0.125 0.092 1.000000e+00
## GABRP 5.417472e-01 -1.185944 0.167 0.119 1.000000e+00
## C1orf174 5.429161e-01 -1.934372 0.125 0.165 1.000000e+00
## WDTC1 5.431408e-01 -1.671781 0.333 0.248 1.000000e+00
## CBFA2T2 5.436034e-01 1.172679 0.292 0.275 1.000000e+00
## HTR7L 5.443228e-01 -1.107350 0.125 0.083 1.000000e+00
## ALG11 5.446579e-01 -1.167865 0.083 0.128 1.000000e+00
## LONRF3 5.447263e-01 -1.654966 0.292 0.330 1.000000e+00
## F2 5.456751e-01 -1.431195 0.167 0.202 1.000000e+00
## ENSGALG00000042984 5.473083e-01 1.126360 0.125 0.092 1.000000e+00
## BCL10 5.478745e-01 1.282677 0.167 0.138 1.000000e+00
## SLC13A3 5.515566e-01 -1.575037 0.083 0.128 1.000000e+00
## ENSGALG00000041225 5.536482e-01 1.428619 0.208 0.183 1.000000e+00
## ENSGALG00000051808 5.548932e-01 -1.162235 0.125 0.092 1.000000e+00
## ENSGALG00000052475 5.570852e-01 -1.109561 0.250 0.211 1.000000e+00
## ENSGALG00000037611 5.582299e-01 1.239232 0.167 0.128 1.000000e+00
## ENSGALG00000048256 5.584980e-01 -1.320579 0.167 0.110 1.000000e+00
## GLYCTK 5.600657e-01 -1.658027 0.125 0.083 1.000000e+00
## ARMC9 5.601601e-01 1.967103 0.375 0.339 1.000000e+00
## ENSGALG00000053678 5.627853e-01 -1.003605 0.208 0.257 1.000000e+00
## GTPBP6 5.658499e-01 -1.000711 0.125 0.174 1.000000e+00
## MOCS1 5.702153e-01 1.593854 0.125 0.092 1.000000e+00
## ENSGALG00000031836 5.710987e-01 -2.252899 0.333 0.431 1.000000e+00
## IL13RA2 5.719839e-01 1.734740 0.208 0.156 1.000000e+00
## CERS3 5.723280e-01 1.706377 0.292 0.266 1.000000e+00
## RFWD3 5.735422e-01 1.288011 0.208 0.174 1.000000e+00
## SMYD2 5.736452e-01 -1.196993 0.417 0.450 1.000000e+00
## TXNRD1 5.741173e-01 -1.082358 0.250 0.183 1.000000e+00
## ORC3 5.755574e-01 1.088173 0.417 0.413 1.000000e+00
## MMAA 5.759611e-01 -1.252673 0.083 0.119 1.000000e+00
## DUSP6 5.759611e-01 -1.632647 0.083 0.119 1.000000e+00
## P3H3 5.795190e-01 1.701131 0.333 0.275 1.000000e+00
## LY75 5.819978e-01 -1.756817 0.125 0.183 1.000000e+00
## ENSGALG00000017040 5.832520e-01 -1.976347 0.083 0.119 1.000000e+00
## CHST1 5.840860e-01 -1.015016 0.125 0.083 1.000000e+00
## MICALL2 5.840860e-01 -2.304276 0.125 0.083 1.000000e+00
## ATIC 5.842325e-01 -1.185351 0.333 0.303 1.000000e+00
## SPPL2A 5.843458e-01 -1.587647 0.125 0.174 1.000000e+00
## ZNRF3 5.852467e-01 1.458998 0.417 0.440 1.000000e+00
## ENSGALG00000054547 5.854854e-01 1.320258 0.125 0.193 1.000000e+00
## NTS 5.857363e-01 1.401829 0.125 0.092 1.000000e+00
## DUSP1 5.865234e-01 -1.579969 0.208 0.147 1.000000e+00
## GRTP1 5.869528e-01 1.076116 0.167 0.138 1.000000e+00
## FAM199X 5.881007e-01 -1.057750 0.208 0.165 1.000000e+00
## LIPT1 5.905857e-01 -1.340554 0.083 0.119 1.000000e+00
## EOMES 5.916091e-01 -2.141917 0.167 0.193 1.000000e+00
## TOB2 5.916246e-01 -1.437180 0.375 0.294 1.000000e+00
## ZFHX4 5.917697e-01 1.452427 0.167 0.128 1.000000e+00
## GLDN 5.935696e-01 2.663816 0.125 0.092 1.000000e+00
## RGR 5.942330e-01 -1.540129 0.208 0.165 1.000000e+00
## ENSGALG00000054434 5.973649e-01 1.419367 0.208 0.193 1.000000e+00
## MCCC2L 5.975427e-01 1.208035 0.208 0.174 1.000000e+00
## RAD51 5.979615e-01 -1.244278 0.083 0.119 1.000000e+00
## GFRA4 6.002599e-01 1.318763 0.167 0.138 1.000000e+00
## PTPRJ 6.002882e-01 1.140527 0.333 0.358 1.000000e+00
## UHRF1BP1 6.005362e-01 1.604351 0.208 0.183 1.000000e+00
## UNC5B 6.008513e-01 1.607136 0.417 0.404 1.000000e+00
## PLEK 6.014506e-01 1.883758 0.125 0.092 1.000000e+00
## RIT1 6.015421e-01 -1.800766 0.125 0.156 1.000000e+00
## LIMD1 6.022253e-01 -1.220842 0.208 0.275 1.000000e+00
## C1H2ORF49 6.048819e-01 -1.200782 0.250 0.294 1.000000e+00
## TRMT5 6.048819e-01 -1.690437 0.250 0.294 1.000000e+00
## ENSGALG00000053827 6.049856e-01 2.383771 0.250 0.229 1.000000e+00
## NFE2L2 6.056966e-01 -1.181479 0.125 0.165 1.000000e+00
## ENSGALG00000052586 6.065846e-01 1.700094 0.125 0.183 1.000000e+00
## CBX8 6.067028e-01 -1.013489 0.333 0.266 1.000000e+00
## ENSGALG00000038810 6.085752e-01 -1.115663 0.125 0.083 1.000000e+00
## SENP1 6.097117e-01 2.434594 0.125 0.193 1.000000e+00
## ENSGALG00000029462 6.100136e-01 -1.101486 0.333 0.275 1.000000e+00
## ZDHHC20 6.128422e-01 -1.326427 0.333 0.413 1.000000e+00
## SRD5A3 6.160540e-01 1.104773 0.250 0.229 1.000000e+00
## DOCK8 6.167033e-01 1.142985 0.375 0.450 1.000000e+00
## AGPS 6.171687e-01 1.659283 0.333 0.349 1.000000e+00
## SUPT7L 6.172275e-01 2.794340 0.125 0.101 1.000000e+00
## DCBLD1 6.172275e-01 1.914819 0.125 0.101 1.000000e+00
## ENSGALG00000044313 6.172275e-01 1.899670 0.125 0.101 1.000000e+00
## SURF2 6.172275e-01 -1.566569 0.083 0.110 1.000000e+00
## ENSGALG00000043074 6.203357e-01 -1.344007 0.083 0.119 1.000000e+00
## ITPRID2 6.204721e-01 1.685105 0.167 0.138 1.000000e+00
## HAUS2 6.210313e-01 1.431823 0.208 0.193 1.000000e+00
## ENSGALG00000048202 6.218562e-01 -1.908312 0.333 0.312 1.000000e+00
## ENSGALG00000051532 6.249838e-01 2.519226 0.125 0.101 1.000000e+00
## SELENOP2 6.251454e-01 -1.555406 0.208 0.147 1.000000e+00
## HHATL 6.275645e-01 -1.404505 0.250 0.284 1.000000e+00
## ENSGALG00000026970 6.279961e-01 -1.446811 0.125 0.156 1.000000e+00
## MLLT1 6.282936e-01 1.373528 0.458 0.532 1.000000e+00
## LATS1 6.307233e-01 1.332014 0.208 0.183 1.000000e+00
## ENSGALG00000048001 6.368282e-01 1.122952 0.417 0.560 1.000000e+00
## NFKB1 6.391930e-01 1.354592 0.417 0.468 1.000000e+00
## PRKDC 6.394066e-01 -1.039728 0.333 0.303 1.000000e+00
## ENSGALG00000052510 6.394066e-01 -1.222488 0.333 0.303 1.000000e+00
## CELF4 6.402179e-01 1.350574 0.167 0.128 1.000000e+00
## ENSGALG00000047455 6.414075e-01 -1.113256 0.125 0.156 1.000000e+00
## EOGT 6.415432e-01 -1.172415 0.125 0.092 1.000000e+00
## ENSGALG00000053304 6.417994e-01 -1.305607 0.292 0.303 1.000000e+00
## TSHZ3 6.429767e-01 1.840107 0.208 0.183 1.000000e+00
## ZBED4 6.439986e-01 -1.273852 0.167 0.211 1.000000e+00
## SCNN1A 6.439986e-01 -1.617414 0.167 0.211 1.000000e+00
## SRF 6.444197e-01 -2.580887 0.125 0.147 1.000000e+00
## SLC44A3 6.445292e-01 1.226836 0.250 0.321 1.000000e+00
## ENSGALG00000054481 6.457215e-01 1.207300 0.250 0.220 1.000000e+00
## ENSGALG00000004676 6.457215e-01 -1.517781 0.208 0.229 1.000000e+00
## GOSR1 6.496032e-01 -1.104786 0.500 0.459 1.000000e+00
## ENSGALG00000053391 6.496939e-01 -1.298495 0.125 0.092 1.000000e+00
## DAB2 6.496939e-01 -1.302630 0.125 0.092 1.000000e+00
## CHRDL1 6.503902e-01 -1.114899 0.208 0.275 1.000000e+00
## ATR 6.514714e-01 1.710292 0.250 0.220 1.000000e+00
## DHRS3 6.536252e-01 1.103014 0.250 0.239 1.000000e+00
## HS3ST4 6.541824e-01 1.054293 0.458 0.468 1.000000e+00
## CACNA1E 6.543300e-01 1.560681 0.333 0.339 1.000000e+00
## DDX47 6.546202e-01 1.026745 0.333 0.284 1.000000e+00
## ENSGALG00000024266 6.564192e-01 2.054858 0.125 0.101 1.000000e+00
## ENSGALG00000049244 6.564192e-01 -1.107087 0.125 0.101 1.000000e+00
## ENSGALG00000052050 6.573695e-01 -1.630880 0.292 0.303 1.000000e+00
## CIB3 6.584107e-01 1.111641 0.083 0.119 1.000000e+00
## RNASET2 6.584308e-01 -1.362813 0.167 0.193 1.000000e+00
## GNLY 6.585691e-01 -1.124886 0.250 0.266 1.000000e+00
## MSN 6.615395e-01 -1.090682 0.208 0.183 1.000000e+00
## MAF1 6.617447e-01 1.133470 0.167 0.147 1.000000e+00
## RAB40C 6.617546e-01 1.052362 0.292 0.266 1.000000e+00
## PRDM11 6.647905e-01 -1.597683 0.333 0.266 1.000000e+00
## C1orf112 6.649250e-01 1.203240 0.292 0.229 1.000000e+00
## ENSGALG00000053619 6.651565e-01 -1.223426 0.333 0.284 1.000000e+00
## IGDCC3 6.657786e-01 1.021219 0.208 0.174 1.000000e+00
## ENSGALG00000043352 6.668548e-01 1.615864 0.375 0.495 1.000000e+00
## LIN54 6.669200e-01 1.228159 0.250 0.229 1.000000e+00
## ARNTL2 6.673837e-01 2.134446 0.542 0.578 1.000000e+00
## ENSGALG00000049933 6.677744e-01 1.646562 0.208 0.183 1.000000e+00
## AMACR 6.688448e-01 -1.368066 0.208 0.229 1.000000e+00
## TCP11X2 6.722756e-01 -1.130468 0.417 0.440 1.000000e+00
## ENSGALG00000043725 6.723727e-01 1.106212 0.125 0.101 1.000000e+00
## FBXO30 6.723727e-01 1.038496 0.125 0.101 1.000000e+00
## GALNT13 6.725046e-01 1.034890 0.583 0.578 1.000000e+00
## TXNDC11 6.730897e-01 -1.638268 0.333 0.294 1.000000e+00
## INO80D 6.741507e-01 1.802927 0.125 0.174 1.000000e+00
## LZTS3 6.743953e-01 -2.037938 0.125 0.092 1.000000e+00
## HPGD 6.743953e-01 -2.349144 0.125 0.092 1.000000e+00
## ENSGALG00000016570 6.804057e-01 1.095913 0.125 0.101 1.000000e+00
## TCIRG1 6.804057e-01 -1.605390 0.083 0.110 1.000000e+00
## ENSGALG00000029375 6.804057e-01 -1.710814 0.083 0.110 1.000000e+00
## LRRC74B 6.818952e-01 -1.369072 0.250 0.183 1.000000e+00
## ITGBL1 6.823346e-01 -2.040619 0.125 0.156 1.000000e+00
## FEM1C 6.827094e-01 -1.139990 0.125 0.092 1.000000e+00
## GLRA4 6.830131e-01 -1.309160 0.167 0.128 1.000000e+00
## ENSGALG00000009458 6.836503e-01 2.903746 0.250 0.312 1.000000e+00
## ARSB 6.837257e-01 1.648654 0.292 0.275 1.000000e+00
## ENSGALG00000054099 6.865852e-01 -1.044928 0.375 0.404 1.000000e+00
## SLC41A1 6.884751e-01 -1.556621 0.083 0.110 1.000000e+00
## ENSGALG00000050680 6.895280e-01 1.237452 0.083 0.119 1.000000e+00
## ZDHHC18 6.898714e-01 1.210751 0.167 0.138 1.000000e+00
## MCM8 6.906608e-01 1.191472 0.625 0.651 1.000000e+00
## SLC22A16 6.934562e-01 2.989803 0.125 0.110 1.000000e+00
## COBLL1 6.938974e-01 -1.111332 0.417 0.394 1.000000e+00
## GREM2 6.940743e-01 -2.253266 0.208 0.156 1.000000e+00
## ENSGALG00000016702 6.944740e-01 -1.304870 0.292 0.257 1.000000e+00
## ENSGALG00000038964 6.965801e-01 -1.520969 0.083 0.110 1.000000e+00
## ENSGALG00000004646 6.974096e-01 -1.288147 0.625 0.514 1.000000e+00
## ENSGALG00000031737 6.986247e-01 1.863901 0.167 0.211 1.000000e+00
## ENSGALG00000051228 6.986247e-01 -1.605806 0.167 0.211 1.000000e+00
## ARHGAP8 6.996402e-01 1.676238 0.208 0.257 1.000000e+00
## ENSGALG00000052715 7.013373e-01 2.227602 0.125 0.110 1.000000e+00
## CHTF18 7.031637e-01 1.468971 0.167 0.147 1.000000e+00
## ENSGALG00000035047 7.036618e-01 -1.067103 0.083 0.101 1.000000e+00
## MYOM2 7.040944e-01 -1.744050 0.167 0.138 1.000000e+00
## SFMBT1 7.041874e-01 1.123492 0.333 0.312 1.000000e+00
## ENSGALG00000035244 7.047199e-01 -1.220506 0.083 0.110 1.000000e+00
## SORT1 7.050626e-01 1.528989 0.250 0.239 1.000000e+00
## LARP4B 7.054650e-01 1.228824 0.292 0.284 1.000000e+00
## GLRA1 7.064688e-01 1.465104 0.167 0.229 1.000000e+00
## PAK1 7.101080e-01 -1.370968 0.458 0.468 1.000000e+00
## IL17RA 7.101575e-01 1.287503 0.167 0.147 1.000000e+00
## ENSGALG00000035035 7.114775e-01 1.033897 0.292 0.367 1.000000e+00
## ENSGALG00000003464 7.128937e-01 1.472646 0.125 0.101 1.000000e+00
## RBPMS2 7.132192e-01 1.137117 0.083 0.119 1.000000e+00
## ENPP2 7.138832e-01 -1.557843 0.250 0.284 1.000000e+00
## JCAD 7.163434e-01 -2.582547 0.125 0.092 1.000000e+00
## ENSGALG00000053725 7.166890e-01 1.068457 0.250 0.239 1.000000e+00
## RPUSD4 7.168238e-01 1.068927 0.208 0.284 1.000000e+00
## PARS2 7.171956e-01 1.310667 0.125 0.110 1.000000e+00
## PSKH1 7.184217e-01 1.029177 0.167 0.138 1.000000e+00
## SLC4A1AP 7.194164e-01 -1.216132 0.250 0.284 1.000000e+00
## TAF5L 7.203169e-01 1.248858 0.208 0.202 1.000000e+00
## KCNB2 7.208198e-01 1.112757 0.458 0.624 1.000000e+00
## ENSGALG00000055077 7.211796e-01 1.739966 0.083 0.119 1.000000e+00
## ENSGALG00000009156 7.211796e-01 1.504119 0.083 0.119 1.000000e+00
## PTPN2 7.217106e-01 1.299995 0.292 0.367 1.000000e+00
## TM7SF3 7.225311e-01 1.476534 0.333 0.349 1.000000e+00
## ENSGALG00000048500 7.251711e-01 1.565659 0.125 0.110 1.000000e+00
## CERCAM 7.251711e-01 1.522783 0.125 0.110 1.000000e+00
## ENSGALG00000033055 7.265583e-01 -1.319513 0.542 0.495 1.000000e+00
## ENSGALG00000051354 7.289031e-01 -1.205727 0.417 0.523 1.000000e+00
## FAM46A 7.295779e-01 1.153139 0.167 0.156 1.000000e+00
## ENSGALG00000040205 7.312837e-01 -1.568164 0.167 0.147 1.000000e+00
## RNF4 7.331766e-01 1.006827 0.125 0.110 1.000000e+00
## ENSGALG00000006143 7.341574e-01 -1.040699 0.375 0.376 1.000000e+00
## TMEM56 7.341574e-01 -1.062390 0.375 0.376 1.000000e+00
## RNF166 7.345558e-01 -1.295555 0.167 0.193 1.000000e+00
## RECQL 7.364705e-01 -1.175565 0.125 0.147 1.000000e+00
## ENSGALG00000047972 7.367666e-01 1.386574 0.250 0.266 1.000000e+00
## UPF1 7.372774e-01 1.036044 0.542 0.541 1.000000e+00
## RAB20 7.376102e-01 -1.164608 0.083 0.110 1.000000e+00
## COMTD1 7.383723e-01 1.299923 0.167 0.147 1.000000e+00
## MAU2 7.406568e-01 -1.085714 0.333 0.394 1.000000e+00
## BMPR1B 7.410316e-01 -1.966538 0.167 0.193 1.000000e+00
## DHODH 7.425294e-01 -1.625174 0.208 0.165 1.000000e+00
## UBE3B 7.437316e-01 -1.335025 0.125 0.147 1.000000e+00
## RXFP1 7.439845e-01 -1.291291 0.250 0.294 1.000000e+00
## ENSGALG00000052590 7.449474e-01 -1.588046 0.458 0.459 1.000000e+00
## ENSGALG00000041312 7.451102e-01 1.376724 0.333 0.330 1.000000e+00
## ENSGALG00000052144 7.454831e-01 1.160195 0.125 0.156 1.000000e+00
## ENSGALG00000047799 7.454831e-01 -1.176794 0.167 0.147 1.000000e+00
## FDXACB1 7.459111e-01 -1.129755 0.083 0.110 1.000000e+00
## LRP4 7.460386e-01 1.662239 0.250 0.239 1.000000e+00
## ELOF1 7.462344e-01 -1.027837 0.083 0.101 1.000000e+00
## CACNG3 7.483088e-01 -1.274836 0.458 0.404 1.000000e+00
## ENSGALG00000034591 7.510155e-01 -1.279245 0.125 0.147 1.000000e+00
## GRIN2B 7.512127e-01 1.688155 0.625 0.725 1.000000e+00
## ANO2 7.516834e-01 -1.044028 0.250 0.303 1.000000e+00
## SDAD1 7.541972e-01 -1.037459 0.625 0.532 1.000000e+00
## ENSGALG00000048091 7.542417e-01 1.435382 0.083 0.110 1.000000e+00
## SIMC1 7.542417e-01 -1.620424 0.083 0.110 1.000000e+00
## LAMC1 7.544452e-01 1.810762 0.292 0.294 1.000000e+00
## NENF 7.548476e-01 -1.355510 0.083 0.101 1.000000e+00
## UNG 7.548476e-01 -1.576150 0.083 0.101 1.000000e+00
## ENSGALG00000021611 7.555068e-01 -1.351748 0.375 0.440 1.000000e+00
## RFX4 7.564743e-01 1.534880 0.458 0.505 1.000000e+00
## ENSGALG00000004604 7.573008e-01 2.241789 0.208 0.183 1.000000e+00
## TERB1 7.574632e-01 1.042378 0.167 0.156 1.000000e+00
## PLAGL2 7.634913e-01 -1.567280 0.083 0.101 1.000000e+00
## SCNN1G 7.640885e-01 -1.133625 0.125 0.138 1.000000e+00
## C1orf159 7.642380e-01 1.703694 0.292 0.358 1.000000e+00
## ENSGALG00000029930 7.664125e-01 -1.829058 0.250 0.248 1.000000e+00
## SPNS3 7.669439e-01 1.633625 0.125 0.156 1.000000e+00
## MAD2L2 7.692860e-01 -1.210216 0.542 0.468 1.000000e+00
## ENSGALG00000047988 7.709880e-01 -1.557584 0.125 0.101 1.000000e+00
## FLVCR1 7.721647e-01 -1.283909 0.083 0.101 1.000000e+00
## ORMDL3 7.721647e-01 -1.289839 0.083 0.101 1.000000e+00
## NEDD1 7.721647e-01 -1.314787 0.083 0.101 1.000000e+00
## CAMK2A 7.770089e-01 1.027845 0.208 0.211 1.000000e+00
## CHEK1 7.794019e-01 1.281830 0.083 0.110 1.000000e+00
## LRRC42 7.794019e-01 -1.152949 0.125 0.101 1.000000e+00
## TMEM69 7.797278e-01 -1.185500 0.167 0.174 1.000000e+00
## LAPTM4B 7.816836e-01 -1.023398 0.667 0.670 1.000000e+00
## UNC45A 7.817927e-01 1.237058 0.125 0.110 1.000000e+00
## ATP13A1 7.834111e-01 -1.271317 0.167 0.183 1.000000e+00
## LDB3 7.838695e-01 1.356816 0.458 0.459 1.000000e+00
## KIF9 7.846171e-01 1.617620 0.417 0.450 1.000000e+00
## GPD1L 7.878038e-01 1.464910 0.333 0.330 1.000000e+00
## LRRC41 7.878418e-01 1.085611 0.083 0.110 1.000000e+00
## CYP2J21 7.878418e-01 1.016258 0.083 0.110 1.000000e+00
## ENG 7.885849e-01 -2.047919 0.167 0.147 1.000000e+00
## KLHDC10 7.895961e-01 -1.079250 0.083 0.101 1.000000e+00
## ENSGALG00000048053 7.927647e-01 1.626349 0.167 0.156 1.000000e+00
## ERO1B 7.963155e-01 1.940037 0.208 0.202 1.000000e+00
## CTSS 7.983520e-01 2.175488 0.083 0.101 1.000000e+00
## ENSGALG00000000295 8.031046e-01 2.039180 0.125 0.156 1.000000e+00
## CLCA1 8.047954e-01 1.005190 0.083 0.110 1.000000e+00
## FCHSD1 8.063117e-01 -1.171508 0.167 0.138 1.000000e+00
## ENSGALG00000046907 8.064378e-01 -1.352449 0.125 0.110 1.000000e+00
## ENSGALG00000015026 8.064378e-01 -1.760097 0.125 0.110 1.000000e+00
## H6PD 8.071334e-01 -1.272417 0.083 0.101 1.000000e+00
## ENSGALG00000008628 8.094954e-01 -1.207223 0.125 0.138 1.000000e+00
## TANK 8.100362e-01 -1.261192 0.125 0.147 1.000000e+00
## NOL6 8.119836e-01 1.734416 0.333 0.349 1.000000e+00
## ENSGALG00000039995 8.119836e-01 -1.129699 0.333 0.349 1.000000e+00
## GAL3ST1 8.133074e-01 -1.268615 0.125 0.101 1.000000e+00
## SVIL 8.134838e-01 1.002807 0.333 0.321 1.000000e+00
## ENSGALG00000053163 8.146971e-01 1.993032 0.125 0.110 1.000000e+00
## PODN 8.156533e-01 1.154148 0.208 0.202 1.000000e+00
## RNF170 8.159169e-01 -1.455382 0.250 0.257 1.000000e+00
## NHEJ1 8.161070e-01 1.136962 0.333 0.339 1.000000e+00
## FUNDC2 8.171306e-01 -1.334900 0.125 0.138 1.000000e+00
## TAB2 8.176926e-01 1.762485 0.125 0.156 1.000000e+00
## ADSL 8.195731e-01 1.135487 0.250 0.275 1.000000e+00
## ATP11C 8.202814e-01 -1.111356 0.292 0.303 1.000000e+00
## CERS1 8.236653e-01 1.484800 0.250 0.266 1.000000e+00
## GSE1 8.247831e-01 -1.014738 0.125 0.138 1.000000e+00
## CD2AP 8.300844e-01 1.261342 0.292 0.312 1.000000e+00
## DHRS11 8.315151e-01 -1.183749 0.292 0.303 1.000000e+00
## COLQ 8.318859e-01 1.293779 0.125 0.119 1.000000e+00
## TBC1D13 8.323440e-01 -1.053590 0.167 0.147 1.000000e+00
## FKBP14 8.324524e-01 -1.233249 0.125 0.138 1.000000e+00
## ENSGALG00000004078 8.336195e-01 1.897253 0.083 0.101 1.000000e+00
## ENSGALG00000038727 8.356879e-01 1.251891 0.167 0.156 1.000000e+00
## GRM7 8.389211e-01 -1.071345 0.750 0.761 1.000000e+00
## RELN 8.392900e-01 -1.326258 0.833 0.780 1.000000e+00
## ANKEF1 8.395457e-01 1.498781 0.208 0.220 1.000000e+00
## ABCC6 8.405996e-01 1.587657 0.208 0.211 1.000000e+00
## ZBTB8OS 8.414894e-01 -1.601559 0.292 0.275 1.000000e+00
## RAP2B 8.416624e-01 -1.179898 0.167 0.174 1.000000e+00
## SETD7 8.416629e-01 -1.172435 0.167 0.174 1.000000e+00
## COL18A1 8.424919e-01 1.453705 0.083 0.101 1.000000e+00
## MOGAT1 8.428934e-01 -1.424507 0.167 0.156 1.000000e+00
## LIN28B 8.456780e-01 1.905429 0.250 0.257 1.000000e+00
## ENSGALG00000053139 8.470539e-01 1.071088 0.125 0.156 1.000000e+00
## GNA11 8.482450e-01 1.072075 0.375 0.477 1.000000e+00
## GFAP 8.483699e-01 -1.649598 0.250 0.239 1.000000e+00
## AFAP1 8.502794e-01 1.355920 0.417 0.550 1.000000e+00
## PIK3IP1 8.513844e-01 1.384677 0.083 0.101 1.000000e+00
## ENSGALG00000048897 8.513844e-01 1.168602 0.083 0.101 1.000000e+00
## AARS2 8.529441e-01 -2.474510 0.167 0.165 1.000000e+00
## PLXNC1 8.535758e-01 1.625541 0.167 0.193 1.000000e+00
## ENSGALG00000016802 8.550426e-01 1.033818 0.125 0.147 1.000000e+00
## SLC49A3 8.558748e-01 -1.451141 0.208 0.239 1.000000e+00
## ENSGALG00000002350 8.568676e-01 -1.629483 0.333 0.367 1.000000e+00
## FBXO31 8.573070e-01 -1.548452 0.250 0.294 1.000000e+00
## INTS3 8.584613e-01 1.021809 0.583 0.606 1.000000e+00
## ENSGALG00000050913 8.585053e-01 1.358295 0.333 0.330 1.000000e+00
## PRTG 8.594931e-01 1.462270 0.333 0.339 1.000000e+00
## CEP120 8.611940e-01 1.041616 0.208 0.202 1.000000e+00
## KLF6 8.618172e-01 -1.104645 0.167 0.147 1.000000e+00
## APBB1 8.625482e-01 1.344317 0.167 0.174 1.000000e+00
## ENSGALG00000047352 8.625940e-01 -1.506294 0.125 0.147 1.000000e+00
## FAN1 8.636439e-01 1.141452 0.250 0.257 1.000000e+00
## BEAN1 8.675489e-01 1.373110 0.250 0.330 1.000000e+00
## DCAF12L2 8.688453e-01 -1.229568 0.292 0.284 1.000000e+00
## ENSGALG00000054172 8.704415e-01 -1.460276 0.208 0.183 1.000000e+00
## RBM41 8.710240e-01 -1.014301 0.125 0.138 1.000000e+00
## ENSGALG00000045093 8.710240e-01 -1.111396 0.125 0.138 1.000000e+00
## ENSGALG00000047591 8.717505e-01 -1.210970 0.167 0.183 1.000000e+00
## LCAT 8.718035e-01 -2.755066 0.125 0.128 1.000000e+00
## RNF144B 8.728948e-01 1.422080 0.250 0.239 1.000000e+00
## ENSGALG00000054412 8.728948e-01 1.420768 0.208 0.248 1.000000e+00
## DOK4 8.730400e-01 -1.169520 0.125 0.110 1.000000e+00
## TM9SF4 8.744316e-01 1.148185 0.333 0.385 1.000000e+00
## ENSGALG00000051908 8.805898e-01 1.371376 0.167 0.193 1.000000e+00
## SLC12A1 8.805948e-01 1.370874 0.292 0.312 1.000000e+00
## ENSGALG00000051798 8.816805e-01 1.137140 0.250 0.257 1.000000e+00
## NUDT13 8.829538e-01 1.197035 0.458 0.505 1.000000e+00
## GAL 8.835300e-01 1.943545 0.167 0.174 1.000000e+00
## MAD1L1 8.847793e-01 1.061566 0.583 0.752 1.000000e+00
## CRADD 8.862411e-01 1.141157 0.292 0.312 1.000000e+00
## ENSGALG00000006840 8.871325e-01 1.203639 0.083 0.101 1.000000e+00
## RRM2B 8.877069e-01 -1.037712 0.250 0.257 1.000000e+00
## SLC25A19 8.877143e-01 1.902599 0.125 0.128 1.000000e+00
## AS3MT 8.877143e-01 -1.504899 0.125 0.128 1.000000e+00
## KRIT1 8.879008e-01 -1.027044 0.500 0.495 1.000000e+00
## ENSGALG00000053608 8.905429e-01 1.472672 0.167 0.174 1.000000e+00
## BMI1 8.913703e-01 -1.490114 0.250 0.239 1.000000e+00
## CHAF1A 8.916382e-01 -1.362879 0.458 0.422 1.000000e+00
## IL12RB2 8.922540e-01 -1.043773 0.208 0.211 1.000000e+00
## GFRAL 8.922540e-01 -1.565666 0.208 0.211 1.000000e+00
## PCNX4 8.929183e-01 2.455985 0.125 0.147 1.000000e+00
## PTPRK 8.941272e-01 -1.229053 0.708 0.725 1.000000e+00
## ENSGALG00000042485 8.961084e-01 1.218963 0.083 0.101 1.000000e+00
## BTBD7 8.963625e-01 1.242170 0.375 0.486 1.000000e+00
## ENSGALG00000040063 8.970086e-01 2.490048 0.125 0.119 1.000000e+00
## C3H6orf120 8.970086e-01 1.024517 0.125 0.119 1.000000e+00
## CWC22 8.970900e-01 -1.016594 0.333 0.330 1.000000e+00
## TACR2 8.982767e-01 -2.065265 0.125 0.110 1.000000e+00
## ETV1 8.993120e-01 -1.251286 0.167 0.183 1.000000e+00
## SAP30L 8.998001e-01 1.020073 0.375 0.358 1.000000e+00
## DRP2 9.015856e-01 -1.608604 0.292 0.294 1.000000e+00
## ENSGALG00000009628 9.021083e-01 1.313950 0.125 0.138 1.000000e+00
## ENSGALG00000017290 9.027530e-01 -1.118901 0.458 0.440 1.000000e+00
## MIS18A 9.028447e-01 -1.027015 0.167 0.165 1.000000e+00
## C12orf43 9.036704e-01 1.418499 0.125 0.128 1.000000e+00
## IL15 9.045939e-01 2.008641 0.167 0.174 1.000000e+00
## SERPINF1 9.045939e-01 -1.586213 0.167 0.174 1.000000e+00
## ZNF318 9.056897e-01 -1.011560 0.458 0.450 1.000000e+00
## DPP8 9.081423e-01 1.726922 0.125 0.147 1.000000e+00
## DCDC2 9.081423e-01 -1.116447 0.125 0.147 1.000000e+00
## ZMYM3 9.099048e-01 2.026738 0.125 0.138 1.000000e+00
## LMX1B 9.105016e-01 1.555465 0.458 0.514 1.000000e+00
## NECTIN3 9.109155e-01 -1.261596 0.250 0.248 1.000000e+00
## LHFPL2 9.121040e-01 -1.006429 0.417 0.495 1.000000e+00
## ENSGALG00000052885 9.134162e-01 1.113383 0.125 0.119 1.000000e+00
## SCLY 9.138368e-01 -1.540150 0.167 0.147 1.000000e+00
## DIXDC1 9.140489e-01 1.114654 0.208 0.229 1.000000e+00
## UBA5 9.193621e-01 -1.490671 0.250 0.211 1.000000e+00
## BCAS1 9.196655e-01 -1.630942 0.125 0.128 1.000000e+00
## ENSGALG00000045898 9.203488e-01 1.165160 0.250 0.284 1.000000e+00
## PRKG2 9.203937e-01 1.554698 0.208 0.229 1.000000e+00
## ENSGALG00000051548 9.213037e-01 -1.575933 0.167 0.147 1.000000e+00
## SLC35F3 9.227623e-01 2.205533 0.333 0.394 1.000000e+00
## ENSGALG00000034587 9.250578e-01 -1.200122 0.417 0.431 1.000000e+00
## ENSGALG00000054741 9.257082e-01 1.095402 0.417 0.450 1.000000e+00
## SVEP1 9.259112e-01 -2.019052 0.292 0.312 1.000000e+00
## MORN4 9.262265e-01 -1.800162 0.292 0.239 1.000000e+00
## ENSGALG00000047108 9.269956e-01 -1.806094 0.167 0.183 1.000000e+00
## COX16 9.321388e-01 -1.019536 0.500 0.477 1.000000e+00
## ZDHHC16 9.354266e-01 1.104567 0.250 0.248 1.000000e+00
## ENSGALG00000036848 9.354500e-01 -1.085448 0.208 0.220 1.000000e+00
## ALPK3 9.387365e-01 -1.178483 0.167 0.165 1.000000e+00
## VIPR1 9.392956e-01 1.119559 0.875 0.862 1.000000e+00
## SPATA22 9.398427e-01 1.889191 0.167 0.174 1.000000e+00
## ENSGALG00000050884 9.409004e-01 -1.091280 0.250 0.239 1.000000e+00
## HBE 9.411703e-01 -2.029412 0.125 0.138 1.000000e+00
## NTN1 9.418930e-01 2.024274 0.208 0.220 1.000000e+00
## APOH 9.421949e-01 1.168306 0.250 0.257 1.000000e+00
## ENSGALG00000050732 9.433407e-01 1.308227 0.208 0.239 1.000000e+00
## WASF1 9.437173e-01 1.328259 0.125 0.128 1.000000e+00
## SLC22A18 9.443699e-01 -1.243928 0.208 0.211 1.000000e+00
## BCORL1 9.463354e-01 -1.024434 0.125 0.119 1.000000e+00
## PTAR1 9.478183e-01 1.898159 0.167 0.183 1.000000e+00
## CMTR1 9.478183e-01 1.498075 0.167 0.183 1.000000e+00
## RIT2 9.482706e-01 -1.355239 0.250 0.257 1.000000e+00
## ENSGALG00000051993 9.494613e-01 -1.236400 0.208 0.193 1.000000e+00
## ENSGALG00000047572 9.517470e-01 1.530218 0.125 0.128 1.000000e+00
## ZBTB26 9.517470e-01 -2.497857 0.125 0.128 1.000000e+00
## GSN 9.522167e-01 -1.348737 0.500 0.477 1.000000e+00
## ENSGALG00000047971 9.539793e-01 3.271770 0.167 0.174 1.000000e+00
## EXOC3L1 9.548220e-01 1.077353 0.250 0.266 1.000000e+00
## SMTN 9.569249e-01 1.340520 0.208 0.257 1.000000e+00
## WDR76 9.597817e-01 1.636669 0.125 0.128 1.000000e+00
## NHLRC3 9.628330e-01 -1.246704 0.125 0.119 1.000000e+00
## CYP2AB5 9.649262e-01 3.082325 0.208 0.229 1.000000e+00
## UBTD2 9.678029e-01 -1.137954 0.292 0.284 1.000000e+00
## ZNF518A 9.678205e-01 -1.119681 0.125 0.128 1.000000e+00
## CDH5 9.731938e-01 -1.195141 0.583 0.560 1.000000e+00
## DMC1 9.742614e-01 -1.435086 0.167 0.156 1.000000e+00
## CCDC149 9.750792e-01 -1.018784 0.333 0.339 1.000000e+00
## RBM24 9.758625e-01 -1.016460 0.125 0.128 1.000000e+00
## NMNAT1 9.793465e-01 -1.290549 0.125 0.119 1.000000e+00
## PRUNE1 9.796888e-01 -1.139083 0.333 0.284 1.000000e+00
## LOXL1 9.840533e-01 -1.136946 0.250 0.220 1.000000e+00
## ENSGALG00000003074 9.849335e-01 1.158205 0.250 0.266 1.000000e+00
## POMT1 9.849335e-01 1.116905 0.250 0.266 1.000000e+00
## ENSGALG00000046769 9.867136e-01 -1.715114 0.208 0.202 1.000000e+00
## ENSGALG00000048082 9.882238e-01 1.198294 0.125 0.138 1.000000e+00
## ENSGALG00000047181 9.883927e-01 1.097907 0.292 0.294 1.000000e+00
## ATP13A4 9.898858e-01 2.005833 0.167 0.202 1.000000e+00
## ENSGALG00000032717 9.901752e-01 -1.722643 0.208 0.211 1.000000e+00
## SLC39A13 9.912944e-01 -1.402647 0.333 0.284 1.000000e+00
## ENSGALG00000042484 9.915001e-01 -1.585959 0.125 0.110 1.000000e+00
## ENSGALG00000053194 9.933566e-01 -1.234918 0.208 0.202 1.000000e+00
## SEMA3A 9.951253e-01 1.003914 0.542 0.569 1.000000e+00
## ODC1 9.963224e-01 -1.561285 0.167 0.156 1.000000e+00
## ENSGALG00000036191 9.965755e-01 2.468138 0.167 0.193 1.000000e+00
## WAC 9.968105e-01 1.480600 0.208 0.229 1.000000e+00
## ZNF512B 9.972847e-01 -1.396927 0.375 0.358 1.000000e+00
## ZNF804B 9.975164e-01 1.686652 0.417 0.532 1.000000e+00
## ENSGALG00000050453 1.000000e+00 1.627169 0.125 0.138 1.000000e+00
## ENSGALG00000002463 1.000000e+00 1.492626 0.375 0.495 1.000000e+00
## MCOLN3 1.000000e+00 1.159466 0.167 0.174 1.000000e+00
## IGDCC4 1.000000e+00 -1.180044 0.125 0.128 1.000000e+00
## ADGRL4 1.000000e+00 -1.239320 0.375 0.431 1.000000e+00
## TMBIM6 1.000000e+00 -1.514526 0.167 0.156 1.000000e+00FeaturePlot(ex0,rownames(m)[1:12])
Rename parts of ex0 in ex.
Idents(ex,cells=WhichCells(ex0,idents='ex_0'))='ex_0_0'
Idents(ex,cells=WhichCells(ex0,idents='ex_1'))='ex_0_1'
ex$merged.res.3.m<-Idents(ex)
#save(ex,file='ex.RData')DimPlot(ex,group.by = 'merged.res.3.m')
Now look at the interposed like TBR1- group
IN<-subset(ex,idents=c("ex_5","ex_13","ex_15"))IN <- FindVariableFeatures(object = IN,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
IN <- ScaleData(object = IN,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrix#ex <- RunPCA(object = ex,npcs = 30,verbose = FALSE)IN<-RunTruncatedPCA(IN,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: SHISAL2A, OPRM1, RAB3B, OCA2, STMN1, PTN, PAX5, GAPDH, TRPM3, ENSGALG00000054929
## SCN3B, GRM3, CALB2, MDGA1, TAC1, ENSGALG00000053114, KLHL14, TPI1, PAX2, ENSGALG00000051979
## CDH20, SLIT3, ZMYM3, CDH8, C1orf145, ENOPH1, GULP1, ND1, COII, ITGA9
## Negative: ENSGALG00000042339, CACNA2D3, ENSGALG00000046702, MTUS1, KANK4, ENSGALG00000052781, RASAL2, EPC2, BTBD11, BCLAF1
## FAM135A, ENSGALG00000049761, COCH, AGRN, ENSGALG00000048867, ENSGALG00000051765, EPB41L1, MAF, ALDH1L2, PLA2G4A
## NBEAL1, ROR2, BRCA1, AFF4, HABP4, ENSGALG00000048843, ENSGALG00000049634, HEMK1, MCF2, NRIP1
## PC_ 2
## Positive: ENSGALG00000009458, ENSGALG00000050791, NRXN3, CNTFR, ENSGALG00000052555, SORCS2, PDZRN3, MECOM, CRHR2, FAT4
## ENSGALG00000051100, CDH20, MAF, SLIT3, ENSGALG00000003026, ENSGALG00000054301, TENM3, BRWD1, USH2A, ENSGALG00000036877
## ENSGALG00000043352, ENSGALG00000054283, ERBB4, ADARB2, ACSS1, PODXL, EML4, GLA, ENSGALG00000045251, ENSGALG00000055095
## Negative: GAPDH, ROBO2, SOX6, RIT2, ND4, TAFA1, DHCR24, GLIS1, ENSGALG00000053372, PEBP1
## GUCY1A2, EBF2, SNCG, NXPH1, ENSGALG00000003325, ENSGALG00000047891, RALYL, CNTN5, TAFA4, ATP6
## CDH2, ENSGALG00000013155, DYNLRB1, ARMC6, MT-ND2, EBF1, MEGF10, COX3, APOA1, SNTB1
## PC_ 3
## Positive: TAFA1, ENSGALG00000049165, ENSGALG00000047741, ADCY2, DACH2, TAFA4, TRPM3, EFNA5, EBF1, ENSGALG00000047932
## ENOX1, GUCY1A2, KALRN, ROBO2, ENSGALG00000048557, KCNJ6, PLXDC2, PDE5A, ENSGALG00000051088, PTPN21
## ENSGALG00000052732, GLIS1, EBF2, SGCD, ZNF804A, TSPAN4, ARPP21, RCAN1, NXPH1, CALB2
## Negative: CBLN1, ND5, CYTB, LDHB, COII, ND4, MT-CO1, ENSGALG00000043598, SNCG, ND1
## COX3, ATP6, GAPDH, TRAPPC5, SEPTIN11, LRPAP1, CLTC, MAF, EPHA7, ESS2
## MT-ND2, VPS4B, ENSGALG00000054547, DIPK1B, TAF15, CA4, NME5, ENSGALG00000047012, ENSGALG00000006143, CFL2
## PC_ 4
## Positive: PAX5, OPRM1, ENSGALG00000010316, ENSGALG00000029796, GRM3, FOXP2, MDGA1, PDE1C, CALD1, SLIT3
## ENSGALG00000053114, MAML2, OCA2, SHISAL2A, ADARB2, DAAM2, CNKSR2, ENSGALG00000054456, PDZRN3, GABRG3
## UNC5C, PCDH1, FAM13C, ENSGALG00000053975, ADAMTSL1, NRXN3, CDH20, ENSGALG00000053385, EFL1, FGD3
## Negative: NXPH1, ENSGALG00000049233, EBF1, FAM135A, ENSGALG00000006851, TAFA4, SOX6, NPAS3, PLS1, EPHA7
## NHLRC3, NFIB, ENSGALG00000053372, ENSGALG00000047346, PRR14L, ENSGALG00000049811, ACAD11, TAMM41, ENSGALG00000051201, TRAPPC5
## KATNA1, UPP1, CDH4, GRIA1, PTTG2, ZNF518A, ENSGALG00000035051, ENSGALG00000046918, ENSGALG00000053056, RFC3
## PC_ 5
## Positive: LUZP2, ENSGALG00000051949, SGCD, NELL2, ENSGALG00000046455, CNTFR, AGRN, ENSGALG00000009458, CALB2, ENSGALG00000055007
## ENSGALG00000047053, SLIT3, MECOM, PCP4, CYTB, CRHR2, NFIB, PDE1C, ERBB4, ENSGALG00000052781
## AGO2, PLXDC2, ENSGALG00000051186, ENSGALG00000050791, RNF216, ATP6, RBFOX1, BGLAP, ADCY2, ENOX1
## Negative: CHRNA4, ENSGALG00000047891, SASH1, PTPRT, LHFPL2, ENSGALG00000054547, CDC34, ENSGALG00000048090, TSPAN4, TLL1
## ENSGALG00000035051, ADAMTSL1, RBP1, PLCXD3, SPHKAP, ENSGALG00000047213, CBLN1, TMEM196, SNTG2, ENSGALG00000049870
## PTPRK, SHISAL1, KRIT1, ENSGALG00000049055, ENSGALG00000054727, ENSGALG00000054714, ENSGALG00000007110, ENSGALG00000041372, ENSGALG00000052378, CDH8ElbowPlot(object = IN)
#IN <- JackStraw(object = IN, dims=20)
#IN <- ScoreJackStraw(IN,dims=1:20)
#JackStrawPlot(object = IN,dims=1:20)IN<- FindNeighbors(IN,dims=1:11)## Computing nearest neighbor graph## Computing SNNIN <- FindClusters(object = IN, resolution = 3)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 109
## Number of edges: 3344
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.0619
## Number of communities: 35
## Elapsed time: 0 seconds## 17 singletons identified. 18 final clusters.IN <- RunTSNE(object = IN, dims = 1:10, perplexity=20, dim.embed = 2,)
p1<-DimPlot(object = IN, reduction = 'tsne', label=TRUE)
p2<-DimPlot(IN,group.by = 'donor')
p4<-DimPlot(IN,group.by = 'orig.ident')
p5<-FeaturePlot(IN,"nFeature_RNA")
plot_grid(p1,p2,p4,p5,ncol=2)
#FeaturePlot(IN,paste0("PC_",1:20),cols = c("navy", "olivedrab1"))merge clusters using allen package.
rd.dat <- t(GetAssayData(object = IN, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 20)
merge.result <- merge_cl(as.matrix(GetAssayData(object = IN, slot = "data")),
cl = IN$RNA_snn_res.3,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{IN$merged.res.3<-1
} else {
IN$merged.res.3<-paste0("ex_",merge.result$cl)
}p2<-DimPlot(IN,group.by = "merged.res.3",label=T)
p1<-DimPlot(IN,group.by = "RNA_snn_res.3")
p4<-DimPlot(IN,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
Idents(IN)<-'merged.res.3'
m<-FindMarkers(IN,ident.1 = "ex_3",ident.2 = "ex_1",logfc.threshold = 1,min.diff.pct = 0.3)
m## p_val avg_logFC pct.1 pct.2 p_val_adj
## ENSGALG00000053372 4.484829e-08 5.266732 1.00 0.294 0.001092325
## ENSGALG00000048426 4.026555e-07 -3.921338 0.00 0.765 0.009807078
## SLIT3 5.161966e-07 -4.163600 0.52 0.941 0.012572485
## CRHR2 6.758770e-07 -4.140596 0.48 1.000 0.016461659
## ENSGALG00000009458 3.490601e-06 -3.928283 0.44 0.882 0.085017070
## LUZP2 7.444498e-06 -3.814800 0.64 1.000 0.181318196
## ENSGALG00000054547 8.512101e-06 3.865594 0.88 0.353 0.207320740
## ENSGALG00000051949 1.200123e-05 -1.435512 0.64 1.000 0.292301923
## DOCK4 1.350151e-05 -1.640920 0.64 1.000 0.328842726
## FAT4 1.369404e-05 -3.630013 0.40 0.882 0.333532037
## TMEM132C 1.582661e-05 -2.796401 0.56 0.941 0.385472882
## ENSGALG00000054607 2.841301e-05 -3.712883 0.12 0.706 0.692027386
## NPY5R 3.960104e-05 2.567075 0.88 0.412 0.964522861
## ENSGALG00000002287 7.036290e-05 3.536367 0.64 0.000 1.000000000
## NRP1 8.488540e-05 1.923640 0.92 0.471 1.000000000
## LINGO3 9.234858e-05 -3.140341 0.04 0.588 1.000000000
## MPPED2 9.904428e-05 -2.767761 0.32 0.824 1.000000000
## CEP131 1.354049e-04 -2.272286 0.08 0.647 1.000000000
## ENSGALG00000047620 1.495212e-04 2.149130 0.80 0.294 1.000000000
## ENSGALG00000050106 1.988646e-04 -1.141316 0.00 0.471 1.000000000
## TAPBP 1.988646e-04 -3.217357 0.00 0.471 1.000000000
## GDNF 1.988646e-04 -3.476924 0.00 0.471 1.000000000
## COL25A1 2.019322e-04 2.633110 0.76 0.176 1.000000000
## HPCAL1 2.169763e-04 1.430707 0.96 0.647 1.000000000
## IL1RAP 2.953960e-04 -1.745708 0.48 0.882 1.000000000
## ENSGALG00000048719 2.954394e-04 3.947681 0.56 0.000 1.000000000
## DLGAP2 3.515107e-04 1.346341 1.00 0.529 1.000000000
## ENSGALG00000002581 3.856991e-04 3.905958 0.60 0.059 1.000000000
## ENSGALG00000050199 4.038651e-04 2.283655 0.84 0.471 1.000000000
## GRM3 4.911771e-04 -2.051429 0.56 0.882 1.000000000
## XDH 5.039748e-04 -2.373926 0.32 0.765 1.000000000
## ENSGALG00000051388 5.507254e-04 -2.295437 0.08 0.588 1.000000000
## AK5 5.984066e-04 -2.787078 0.20 0.647 1.000000000
## ENSGALG00000051472 6.247582e-04 2.192835 0.80 0.294 1.000000000
## TRAPPC4 6.508454e-04 2.330616 0.84 0.353 1.000000000
## CHRNA4 6.762014e-04 1.398585 0.96 0.529 1.000000000
## ENSGALG00000050135 7.289162e-04 -2.765100 0.12 0.588 1.000000000
## ENSGALG00000047891 7.427285e-04 1.808891 0.92 0.588 1.000000000
## MLLT11 8.345246e-04 1.573449 0.80 0.294 1.000000000
## CHTF18 8.987983e-04 -3.457851 0.04 0.471 1.000000000
## UNC13B 9.803811e-04 2.374002 0.72 0.235 1.000000000
## CDH6 1.122379e-03 -1.301794 0.60 0.941 1.000000000
## ENSGALG00000010316 1.360439e-03 -2.532420 0.48 0.824 1.000000000
## SNCA 1.422876e-03 -1.899630 0.40 0.824 1.000000000
## ENSGALG00000048891 1.451653e-03 1.259300 0.68 0.176 1.000000000
## SCG3 1.471044e-03 2.098047 0.88 0.529 1.000000000
## RXFP1 1.674281e-03 3.564310 0.64 0.235 1.000000000
## ENSGALG00000043225 1.693115e-03 1.433071 0.56 0.059 1.000000000
## NECTIN3 1.693115e-03 -1.667640 0.16 0.647 1.000000000
## COL6A1 1.707986e-03 -1.122512 0.00 0.353 1.000000000
## ADCY2 1.721778e-03 -1.470573 0.64 0.941 1.000000000
## NRDC 1.756274e-03 2.326124 0.88 0.529 1.000000000
## ATP6V1C1 1.800158e-03 1.085384 0.88 0.412 1.000000000
## ENSGALG00000016268 1.965795e-03 1.111064 0.88 0.412 1.000000000
## NETO2 2.151070e-03 1.079300 0.80 0.412 1.000000000
## ENSGALG00000050137 2.362785e-03 -3.205538 0.36 0.706 1.000000000
## ENSGALG00000054978 2.455989e-03 -2.106752 0.04 0.412 1.000000000
## RIT2 2.503781e-03 3.401001 0.72 0.412 1.000000000
## CYGB 2.777741e-03 -2.094329 0.04 0.412 1.000000000
## PPARD 3.046998e-03 -2.566071 0.20 0.647 1.000000000
## SLC2A11 3.106557e-03 2.054419 0.68 0.294 1.000000000
## CADM3 3.253372e-03 2.578247 0.64 0.176 1.000000000
## LMO3 3.320737e-03 -1.922371 0.44 0.765 1.000000000
## ENSGALG00000001476 3.350339e-03 -2.023671 0.20 0.647 1.000000000
## TIMP2 3.536221e-03 1.456103 0.76 0.353 1.000000000
## FAM20B 3.560316e-03 1.963111 0.64 0.176 1.000000000
## SNX17 3.578499e-03 2.025332 0.60 0.118 1.000000000
## ENSGALG00000052669 3.623155e-03 3.712454 0.48 0.059 1.000000000
## SRD5A3 3.744057e-03 3.397887 0.40 0.000 1.000000000
## TIPRL 3.744057e-03 2.007416 0.40 0.000 1.000000000
## ENSGALG00000048122 3.823343e-03 -1.986375 0.12 0.529 1.000000000
## MICAL2 3.893384e-03 2.646666 0.64 0.176 1.000000000
## CD320 4.032510e-03 2.238748 0.68 0.294 1.000000000
## ADAMTS12 4.254516e-03 1.009717 0.60 0.235 1.000000000
## TMEM47 4.356636e-03 1.758925 0.72 0.294 1.000000000
## FAM107A 4.486607e-03 -1.635807 0.04 0.412 1.000000000
## TENT4B 4.505179e-03 1.179244 0.52 0.059 1.000000000
## PTPRQ 4.618569e-03 1.123224 0.80 0.412 1.000000000
## ENSGALG00000053609 4.916665e-03 2.568950 0.60 0.176 1.000000000
## UQCRFS1 5.152858e-03 1.945812 0.68 0.353 1.000000000
## PTK7 5.320847e-03 -1.500896 0.20 0.647 1.000000000
## STX8 5.382302e-03 -1.042058 0.56 0.882 1.000000000
## ENSGALG00000053427 5.947060e-03 3.063854 0.52 0.118 1.000000000
## ENSGALG00000051002 6.089277e-03 1.570696 0.56 0.118 1.000000000
## JCAD 6.457534e-03 -2.537842 0.04 0.353 1.000000000
## ENSGALG00000051100 6.457534e-03 -3.233244 0.04 0.353 1.000000000
## ENSGALG00000030855 6.622999e-03 1.993920 0.76 0.412 1.000000000
## ENSGALG00000043013 6.658314e-03 3.330904 0.36 0.000 1.000000000
## PYCR1 6.658314e-03 3.148470 0.36 0.000 1.000000000
## HAUS6 6.658314e-03 2.638936 0.36 0.000 1.000000000
## GPR149 6.658314e-03 2.027280 0.36 0.000 1.000000000
## ENSGALG00000052860 6.658314e-03 1.347240 0.36 0.000 1.000000000
## PARP4 6.836904e-03 1.386441 0.76 0.353 1.000000000
## DERL1 6.871749e-03 3.848472 0.48 0.118 1.000000000
## CXCL14 6.943220e-03 2.901471 0.44 0.059 1.000000000
## ITGBL1 6.943220e-03 1.311290 0.44 0.059 1.000000000
## RNF128 7.126200e-03 2.318697 0.52 0.118 1.000000000
## ARMC6 7.134685e-03 1.612742 0.64 0.176 1.000000000
## FAM174B 7.272011e-03 -3.181007 0.04 0.353 1.000000000
## SPATA20 7.411547e-03 -3.130991 0.08 0.412 1.000000000
## GOT2 7.457634e-03 1.778227 0.80 0.353 1.000000000
## MT3 7.847062e-03 1.491331 0.68 0.294 1.000000000
## JAM2 7.886047e-03 1.367535 0.68 0.235 1.000000000
## ENSGALG00000051935 8.177504e-03 -2.239211 0.04 0.353 1.000000000
## FGFR3 8.241989e-03 3.003281 0.60 0.176 1.000000000
## NOX4 8.243954e-03 2.782215 0.48 0.118 1.000000000
## DTX2 8.511124e-03 1.941553 0.52 0.118 1.000000000
## TEAD1 8.649893e-03 1.547065 0.56 0.118 1.000000000
## RGS4 9.014722e-03 1.398046 0.88 0.529 1.000000000
## HSPB9 9.436012e-03 2.359439 0.56 0.176 1.000000000
## TTC6 9.436012e-03 1.170048 0.56 0.176 1.000000000
## REEP5 9.887363e-03 1.094554 0.88 0.471 1.000000000
## SHH 1.014138e-02 3.242213 0.56 0.235 1.000000000
## GPR37 1.114389e-02 2.034333 0.56 0.176 1.000000000
## BCL7A 1.114389e-02 -3.192587 0.28 0.588 1.000000000
## ERAP1 1.160522e-02 3.544830 0.32 0.000 1.000000000
## PTGR1 1.160522e-02 2.537006 0.32 0.000 1.000000000
## MRPL53 1.160522e-02 2.446809 0.32 0.000 1.000000000
## ENSGALG00000011608 1.160522e-02 2.419295 0.32 0.000 1.000000000
## STRA8 1.160522e-02 1.853070 0.32 0.000 1.000000000
## SH3BP2 1.160522e-02 1.568209 0.32 0.000 1.000000000
## ENSGALG00000004908 1.173695e-02 1.947828 0.64 0.294 1.000000000
## ENSGALG00000038090 1.174407e-02 1.003377 0.48 0.118 1.000000000
## SLC27A1 1.179507e-02 2.359370 0.40 0.059 1.000000000
## FAM91A1 1.209667e-02 1.974405 0.56 0.176 1.000000000
## STAT3 1.218538e-02 1.707779 0.44 0.059 1.000000000
## ENSGALG00000049430 1.262023e-02 2.266675 0.60 0.235 1.000000000
## SELENOW 1.262023e-02 1.078394 0.60 0.235 1.000000000
## ENSGALG00000050552 1.264631e-02 2.471732 0.52 0.176 1.000000000
## SH3BGRL 1.268282e-02 1.726676 0.76 0.353 1.000000000
## C15H12ORF65 1.270382e-02 1.552928 0.60 0.294 1.000000000
## HSPA2 1.280309e-02 1.721809 0.48 0.118 1.000000000
## SNX16 1.295215e-02 3.121441 0.40 0.059 1.000000000
## CNOT11 1.295215e-02 2.809988 0.40 0.059 1.000000000
## PRICKLE1 1.320761e-02 1.325546 0.64 0.235 1.000000000
## CREB1 1.354066e-02 1.544534 0.72 0.353 1.000000000
## COX10 1.368897e-02 1.188422 0.80 0.412 1.000000000
## ENSGALG00000054380 1.394575e-02 2.485925 0.48 0.118 1.000000000
## MYLIP 1.394575e-02 2.076708 0.48 0.118 1.000000000
## ST8SIA3Z 1.394575e-02 2.069103 0.48 0.118 1.000000000
## ENSGALG00000050152 1.408385e-02 -1.373059 0.40 0.706 1.000000000
## PELO 1.422123e-02 1.290454 0.56 0.176 1.000000000
## ENSGALG00000053975 1.437895e-02 -1.496134 0.40 0.765 1.000000000
## ENSGALG00000051973 1.468715e-02 3.470530 0.44 0.118 1.000000000
## RASSF9 1.517751e-02 2.613401 0.48 0.118 1.000000000
## ENSGALG00000048344 1.517751e-02 1.013706 0.48 0.118 1.000000000
## ENSGALG00000032428 1.540210e-02 1.500948 0.56 0.176 1.000000000
## BLOC1S2 1.557123e-02 2.886355 0.40 0.059 1.000000000
## ANKDD1A 1.600855e-02 2.983699 0.44 0.118 1.000000000
## ENSGALG00000004816 1.650409e-02 3.161603 0.48 0.118 1.000000000
## MTO1 1.650409e-02 1.831346 0.48 0.118 1.000000000
## ERP29 1.666843e-02 1.513631 0.56 0.176 1.000000000
## SH3GL1 1.667212e-02 1.437738 0.68 0.353 1.000000000
## MRPL3 1.667212e-02 1.259792 0.68 0.353 1.000000000
## INVS 1.697279e-02 2.039126 0.68 0.294 1.000000000
## DDX24 1.717211e-02 -1.277605 0.36 0.706 1.000000000
## STRADB 1.743335e-02 2.945977 0.44 0.118 1.000000000
## ENSGALG00000039164 1.802529e-02 -1.275761 0.24 0.647 1.000000000
## NAF1 1.849227e-02 1.256483 0.64 0.294 1.000000000
## ENSGALG00000053993 1.864546e-02 1.879820 0.40 0.059 1.000000000
## OPNVA 1.864546e-02 -1.162358 0.12 0.471 1.000000000
## ENSGALG00000006203 1.896814e-02 3.160781 0.44 0.118 1.000000000
## IRAK1BP1 1.896814e-02 2.658433 0.44 0.118 1.000000000
## PAK1 1.909337e-02 2.717350 0.56 0.235 1.000000000
## TSC22D4 1.946580e-02 2.529292 0.48 0.118 1.000000000
## JMJD8 1.946580e-02 1.837697 0.48 0.118 1.000000000
## ATP11A 1.947792e-02 -2.462009 0.28 0.588 1.000000000
## CEBPG 1.965398e-02 3.641404 0.36 0.059 1.000000000
## CALCA 1.965398e-02 2.754840 0.36 0.059 1.000000000
## TMLHE 1.965398e-02 2.385501 0.36 0.059 1.000000000
## UTP6 1.969525e-02 -1.279926 0.20 0.588 1.000000000
## TMEM196 2.047325e-02 2.951502 0.52 0.176 1.000000000
## TRPM6 2.047325e-02 2.671501 0.52 0.176 1.000000000
## LYPLA1 2.047325e-02 1.647293 0.52 0.176 1.000000000
## ZDHHC2 2.059682e-02 1.760628 0.56 0.235 1.000000000
## DNTT 2.061981e-02 3.214063 0.44 0.118 1.000000000
## TMEM231 2.061981e-02 2.598951 0.44 0.118 1.000000000
## ENSGALG00000046649 2.061981e-02 -1.063926 0.16 0.529 1.000000000
## ENSGALG00000052896 2.138653e-02 -1.194732 0.28 0.647 1.000000000
## CHD1L 2.140128e-02 1.465257 0.64 0.294 1.000000000
## ENSGALG00000003412 2.152871e-02 2.422349 0.36 0.059 1.000000000
## ENSGALG00000003162 2.152871e-02 1.346063 0.36 0.059 1.000000000
## NEK9 2.155450e-02 1.730713 0.60 0.235 1.000000000
## ENSGALG00000030195 2.212450e-02 1.516189 0.52 0.176 1.000000000
## ENSGALG00000052279 2.223814e-02 3.088326 0.40 0.059 1.000000000
## UROS 2.239550e-02 2.720843 0.44 0.118 1.000000000
## MSL3 2.267996e-02 1.225091 0.68 0.294 1.000000000
## COMMD3 2.288172e-02 1.790078 0.48 0.118 1.000000000
## ENSGALG00000050296 2.299977e-02 1.143154 0.64 0.294 1.000000000
## ENSGALG00000050502 2.349924e-02 -1.649653 0.40 0.706 1.000000000
## MPP4 2.389038e-02 1.893356 0.52 0.176 1.000000000
## SETD2 2.409136e-02 1.113600 0.72 0.353 1.000000000
## NRK 2.409222e-02 1.185882 0.84 0.529 1.000000000
## KIF26A 2.420216e-02 2.408414 0.72 0.412 1.000000000
## ENSGALG00000049328 2.420216e-02 1.113198 0.72 0.412 1.000000000
## NCBP3 2.434409e-02 1.634450 0.68 0.294 1.000000000
## ENSGALG00000050371 2.434409e-02 1.146139 0.68 0.294 1.000000000
## SPIRE2 2.481829e-02 -1.377199 0.24 0.588 1.000000000
## ENSGALG00000052823 2.551199e-02 -1.275433 0.16 0.471 1.000000000
## ENSGALG00000052017 2.551199e-02 -2.822555 0.16 0.471 1.000000000
## NCALD 2.574287e-02 2.291570 0.56 0.235 1.000000000
## STARD4 2.574962e-02 2.829111 0.36 0.059 1.000000000
## RNF149 2.574962e-02 2.406747 0.36 0.059 1.000000000
## ENSGALG00000013912 2.574962e-02 1.376071 0.36 0.059 1.000000000
## PDCD11 2.634905e-02 2.806228 0.44 0.118 1.000000000
## LAMTOR2 2.680393e-02 1.039507 0.76 0.412 1.000000000
## CHMP7 2.768931e-02 1.548096 0.56 0.235 1.000000000
## UFM1 2.779164e-02 1.504973 0.52 0.176 1.000000000
## LEPR 2.811628e-02 1.066546 0.36 0.059 1.000000000
## PFDN6 2.854266e-02 1.374437 0.44 0.118 1.000000000
## ENSGALG00000049083 2.884876e-02 -1.019350 0.24 0.588 1.000000000
## ST8SIA6 2.906457e-02 2.056213 0.48 0.176 1.000000000
## HSPH1 2.923915e-02 1.260412 0.80 0.471 1.000000000
## SLC16A3 3.066809e-02 1.864712 0.36 0.059 1.000000000
## MT4 3.079560e-02 1.423032 0.60 0.294 1.000000000
## LRRK1 3.089178e-02 2.439966 0.44 0.118 1.000000000
## RUNDC3A 3.089178e-02 1.709529 0.44 0.118 1.000000000
## NIPA2 3.092796e-02 1.458559 0.60 0.235 1.000000000
## TUBB 3.130026e-02 1.080837 0.48 0.118 1.000000000
## ENSGALG00000049498 3.258596e-02 -1.282509 0.16 0.471 1.000000000
## SYK 3.258596e-02 -2.170651 0.16 0.471 1.000000000
## HMOX2 3.263995e-02 1.298131 0.64 0.294 1.000000000
## ENSGALG00000054557 3.300558e-02 2.688093 0.60 0.294 1.000000000
## ENSGALG00000049634 3.300558e-02 1.105403 0.60 0.294 1.000000000
## ENSGALG00000027056 3.340497e-02 2.866843 0.44 0.118 1.000000000
## POP4 3.340497e-02 1.423114 0.44 0.118 1.000000000
## MED24 3.376960e-02 1.213266 0.48 0.176 1.000000000
## ENSGALG00000028267 3.430784e-02 1.790809 0.56 0.235 1.000000000
## ENSGALG00000041690 3.430784e-02 1.694533 0.56 0.235 1.000000000
## TCP1 3.493651e-02 1.773378 0.64 0.294 1.000000000
## TMEM242 3.493651e-02 -1.489126 0.36 0.706 1.000000000
## ENSGALG00000047181 3.524656e-02 1.085261 0.56 0.176 1.000000000
## KCTD16 3.524656e-02 -1.726348 0.28 0.588 1.000000000
## CEP128 3.524656e-02 -2.280512 0.28 0.588 1.000000000
## MIA3 3.550679e-02 -1.048118 0.64 0.941 1.000000000
## NUDCD2 3.555796e-02 1.163256 0.60 0.235 1.000000000
## NSA2 3.590607e-02 1.319868 0.68 0.353 1.000000000
## PDCD5 3.635689e-02 2.243468 0.48 0.176 1.000000000
## ENSGALG00000029204 3.637250e-02 1.483463 0.36 0.059 1.000000000
## CLK2 3.744224e-02 1.238482 0.84 0.471 1.000000000
## DONSON 3.911124e-02 2.007260 0.48 0.176 1.000000000
## LOXL2 3.911124e-02 1.814105 0.48 0.176 1.000000000
## ENSGALG00000050658 3.923095e-02 1.074722 0.68 0.294 1.000000000
## ENSGALG00000011324 4.002080e-02 2.074678 0.52 0.176 1.000000000
## SLC15A4 4.076674e-02 -1.058188 0.32 0.647 1.000000000
## ANO2 4.343871e-02 -1.022996 0.52 0.824 1.000000000
## ENSGALG00000033591 4.515400e-02 1.483596 0.48 0.176 1.000000000
## ENSGALG00000002350 4.527897e-02 1.065143 0.44 0.118 1.000000000
## KLHL12 4.555101e-02 1.189401 0.64 0.294 1.000000000
## GMPS 4.577394e-02 1.449519 0.80 0.471 1.000000000
## ENSGALG00000045015 4.619876e-02 1.339882 0.60 0.294 1.000000000
## TBC1D12 4.845931e-02 2.153112 0.48 0.176 1.000000000
## C9orf64 4.845931e-02 1.268014 0.48 0.176 1.000000000
## RBFOX3 5.196549e-02 1.632848 0.48 0.176 1.000000000
## CG-16 5.196549e-02 1.239096 0.48 0.176 1.000000000
## NOS1 5.241877e-02 -1.594897 0.40 0.706 1.000000000
## ENSGALG00000051794 5.430628e-02 1.728493 0.76 0.412 1.000000000
## FHL5 5.568142e-02 1.594041 0.48 0.176 1.000000000
## USP46 5.606793e-02 1.122663 0.60 0.294 1.000000000
## SNRNP25 5.606793e-02 1.106382 0.60 0.294 1.000000000
## PCBP2 5.961613e-02 1.539380 0.48 0.176 1.000000000
## ENSGALG00000049955 5.961613e-02 1.474880 0.48 0.176 1.000000000
## CAPN11 5.972493e-02 1.357378 0.60 0.294 1.000000000
## KITLG 5.972597e-02 1.194648 0.68 0.353 1.000000000
## RNFT2 6.082396e-02 -1.782471 0.28 0.588 1.000000000
## PLXNB3 6.377877e-02 1.464307 0.48 0.176 1.000000000
## PDLIM7 6.647824e-02 -1.204609 0.36 0.706 1.000000000
## NAA30 6.763337e-02 1.487730 0.60 0.294 1.000000000
## DOCK5 8.061582e-02 2.139345 0.56 0.235 1.000000000
## ENSGALG00000054676 8.566619e-02 1.895801 0.56 0.235 1.000000000
## PTPN12 9.523737e-02 -1.047841 0.40 0.706 1.000000000
## ENSGALG00000037435 1.035880e-01 1.164225 0.36 0.765 1.000000000
## CASD1 1.512470e-01 -1.176921 0.48 0.176 1.000000000
## AARS2 1.529112e-01 1.110034 0.32 0.647 1.000000000FeaturePlot(IN,rownames(m)[1:12])
Rename parts of IN in ex.
Idents(IN)<-"merged.res.3"
Idents(ex)<-"merged.res.3.m"
Idents(ex,cells=WhichCells(IN,idents='ex_0'))='ex_IN_0'
Idents(ex,cells=WhichCells(IN,idents='ex_1'))='ex_IN_1'
Idents(ex,cells=WhichCells(IN,idents='ex_2'))='ex_IN_2'
Idents(ex,cells=WhichCells(IN,idents='ex_3'))='ex_IN_3'
ex$merged.res.3.m<-Idents(ex)look at main blob of cells.
Idents(ex)<-'merged.res.3'
M<-subset(ex,idents=c("ex_0","ex_5","ex_15","ex_13"),invert=T)M <- FindVariableFeatures(object = M,selection.method = "vst",nfeatures = 2000,verbose=FALSE)
M <- ScaleData(object = M,vars.to.regress = c('nCount_RNA',"donor"))## Regressing out nCount_RNA, donor## Centering and scaling data matrixM<-RunTruncatedPCA(M,n.genes.pc = 60,npcs=30)## PC_ 1
## Positive: KIAA1217, ENSGALG00000052781, SLC24A2, CA6, CCDC85A, LSAMP, CACNA2D3, MAF, SNTG1, NOC4L
## NEFM, EPB41L1, ENSGALG00000006864, PTPRG, KCNG2, GABRG3, ENSGALG00000048048, ENSGALG00000046863, XDH, NEFL
## EIF2B5, ADARB2, ENSGALG00000000816, CNTNAP5, ENSGALG00000049672, PROSER1, KITLG, KIF21B, AFF1, GRAMD1B
## Negative: RAB3B, GAPDH, PDE1C, SCG2, ENSGALG00000048785, SS2, NPTX2, PIEZO2, CACNA1G, KCNIP1
## SCN3B, SYP, SYT10, FXYD6, SRD5A2, ENSGALG00000041622, TRPS1, CXCL14, RIT2, CNR1
## BMP6, LTBP2, PTPRT, GRM7, ANO2, ENSGALG00000053609, CDH13, GLRA2, GRIA1, CDH8
## PC_ 2
## Positive: ADARB2, ZNF804A, NPTX2, ENSGALG00000048785, SEMA6A, RIT2, KCNH8, RUNX2, ALCAM, CACNA2D3
## TMTC2, LHFPL6, CACNA1G, CNR1, HTR4, ENSGALG00000049933, GRM3, ENSGALG00000031713, PLXDC2, SVIL
## MAF, CDH13, ENSGALG00000051354, ENSGALG00000048411, SYT17, ENSGALG00000049321, PDE10A, SS2, MPPED2, CDH8
## Negative: ENSGALG00000049055, TAC1, UTRN, ENSGALG00000047213, DRD2, ENSGALG00000053263, EPHA7, SPON1, RSPO1, CHRNA4
## ADAMTSL1, ZNF423, BCL11B, SEMA5B, ENSGALG00000049731, SEMA3A, GALNTL6, KCNF1, ENSGALG00000050067, SEMA3C
## KCNB2, IL1RAPL2, TLL2, FBXL7, CADM1, SEMA3E, PLXNC1, GRIK1, FAT3, TAFA1
## PC_ 3
## Positive: UNC5C, ENSGALG00000049165, TAFA1, BCL11B, SYT10, IGSF3, NPY5R, ENSGALG00000029613, PCDH10, GLRA2
## KCNIP1, SPON1, PIEZO2, ENSGALG00000047213, SEMA3D, LTBP2, PAX6, SYP, CDH4, ENSGALG00000053609
## CADM1, ADAMTSL1, QKI, ENSGALG00000049055, PDE10A, DRD2, DPYD, ANGPT1, CXCL14, SEMA3C
## Negative: ND4, ND1, CYTB, ENSGALG00000043598, GABRG3, ATP6, MT-CO1, COX3, ND5, CBLN3
## COII, ALDH1A3, ADARB2, CACNA2D3, GAPDH, NEFL, CALB2, VCAN, ENSGALG00000053114, PLK2
## PTPRK, COPS8, ENSGALG00000006864, ENSGALG00000054558, NEFM, ENSGALG00000038652, SEMA6A, KITLG, HYAL1, SPARC
## PC_ 4
## Positive: GALNTL6, THSD7B, ENSGALG00000053114, CALB2, GRIK1, CDH11, CELF2, ITGA9, ENSGALG00000054048, SEMA6A
## ENSGALG00000029796, PTPRK, GABRG3, SEMA5A, GRM3, CBLN3, PLXDC2, GRIA1, VCAN, TENM3
## ENSGALG00000051473, PTPRT, IL1RAP, ENSGALG00000048402, CDH13, CNR1, TRPS1, EPHA7, RSPO3, GULP1
## Negative: CHGB, ENSGALG00000043598, COX3, COII, ND5, ND4, MT-CO1, ATP6, CYTB, LAMP5
## GAPDH, ND1, NEFL, PAX6, ENSGALG00000049165, NPY5R, ENSGALG00000054547, UNC5C, ENSGALG00000054138, COL25A1
## SEMA3D, ENSGALG00000004394, HTR4, CAPN11, LRIG3, TMEM132C, NEFM, COPS8, SPON1, C3H1orf95
## PC_ 5
## Positive: ENSGALG00000049165, TMTC2, ENSGALG00000054547, ENSGALG00000006851, PCDH10, UNC5C, HTR4, COL25A1, CCDC89, ENSGALG00000053399
## ENSGALG00000023083, ENSGALG00000004394, CRHR2, ENSGALG00000054138, ENSGALG00000006864, OPN5L1, ADARB2, SNTG1, RUNX2, TMEM132C
## ENSGALG00000044293, MPPED2, ENSGALG00000053263, ENSGALG00000052572, ENSGALG00000004078, MAD2L1, IL5RA, ENSGALG00000050169, ENSGALG00000033297, CACNA2D3
## Negative: CYTB, ND5, CALB2, MT-CO1, ATP6, ND4, ND1, COII, COX3, GAPDH
## ENSGALG00000043598, NEFL, CDH11, SEMA5A, SKP1, PTPRT, GET4, ACAT1, ATP6V1C1, PDE1C
## NEFM, PLPP2, ENSGALG00000053114, CITED4, SUPT6H, TMED7, ACSBG2, FBXL7, RSPO3, ADAMTSL1ElbowPlot(object = M)
#M <- JackStraw(object = M, dims=20)
#M <- ScoreJackStraw(M,dims=1:20)
#JackStrawPlot(object = M,dims=1:20)M<- FindNeighbors(M,dims=1:9)## Computing nearest neighbor graph## Computing SNNM <- FindClusters(object = M, resolution = 3)## Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck
##
## Number of nodes: 579
## Number of edges: 16776
##
## Running Louvain algorithm...
## Maximum modularity in 10 random starts: 0.5034
## Number of communities: 14
## Elapsed time: 0 secondsM <- RunTSNE(object = M, dims = 1:9, perplexity=30, dim.embed = 2,)
p1<-DimPlot(object = M, reduction = 'tsne', label=TRUE)
p2<-DimPlot(M,group.by = 'donor')
p4<-DimPlot(M,group.by = 'orig.ident')
p5<-FeaturePlot(M,"nFeature_RNA")
plot_grid(p1,p2,p4,p5,ncol=2)
merge clusters using allen package.
rd.dat <- t(GetAssayData(object = M, slot = "scale.data"))
merge.param <- de_param(padj.th = 0.05,
lfc.th = 1,
low.th = 1,
q1.th = 0.4,
q.diff.th = 0.6,
de.score.th = 20)
merge.result <- merge_cl(as.matrix(GetAssayData(object = M, slot = "data")),
cl = M$RNA_snn_res.3,
rd.dat = rd.dat,
de.param = merge.param)
if (is.null(merge.result))
{M$merged.res.3<-1
} else {
M$merged.res.3<-paste0("ex_",merge.result$cl)
}p2<-DimPlot(M,group.by = "merged.res.3",label=T)
p1<-DimPlot(M,group.by = "RNA_snn_res.3")
p4<-DimPlot(M,group.by = 'FACS')
plot_grid(p2,p1,p4,ncol=3)
Idents(ex)<-"merged.res.3.m"
Idents(M)<-'merged.res.3'
Idents(ex,cells=WhichCells(M,idents='ex_0'))='ex_M_0'
Idents(ex,cells=WhichCells(M,idents='ex_1'))='ex_M_1'
Idents(ex,cells=WhichCells(M,idents='ex_2'))='ex_M_2'
Idents(ex,cells=WhichCells(M,idents='ex_3'))='ex_M_3'
Idents(ex,cells=WhichCells(M,idents='ex_4'))='ex_M_4'
Idents(ex,cells=WhichCells(M,idents='ex_5'))='ex_M_5'
Idents(ex,cells=WhichCells(M,idents='ex_6'))='ex_M_6'
Idents(ex,cells=WhichCells(M,idents='ex_7'))='ex_M_7'
Idents(ex,cells=WhichCells(M,idents='ex_9'))='ex_M_8'
Idents(ex,cells=WhichCells(M,idents='ex_10'))='ex_M_9'
Idents(ex,cells=WhichCells(M,idents='ex_13'))='ex_M_10'
ex$merged.res.3.m<-Idents(ex)